Modified polynucleotides and methods of preparing same

CAFC
  • US 5,449,767 A
  • Filed: 05/20/1992
  • Issued: 09/12/1995
  • Est. Priority Date: 04/17/1981
  • Status: Expired due to Term
First Claim
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1. An oligo- or polynucleotide containing a nucleotide having the structure:

  • ##STR26## wherein B represents a 7-deazapurine or a pyrimidine moiety covalently bonded to the C1 '"'"'-position of the sugar moiety, provided that whenever B is a 7-deazapurine, the sugar moiety is attached at the N9 -position of the 7-deazapurine, and whenever B is a pyrimidine, the sugar moiety is attached at the N1 -position of the pyrimidine;

    wherein A comprises at least three carbon atoms and represents at least one component of a signaling moiety capable of producing a detectable signal;

    wherein B and A are covalently attached directly or through a linkage group that does not substantially interfere with the characteristic ability of the oligo- or polynucleotide to hybridize with a nucleic acid and does not substantially interfere with formation of the signalling moiety or detection of the detectable signal, provided also that if B is 7-deazapurine, A or the linkage group is attached to the 7-position of the deazapurine, and if B is pyrimidine, A or the linkage group is attached to the 5-position of the pyrimidine;

    wherein one of x and y represents ##STR27## and the other of x and y is absent or represents --OH or --H; and

    wherein z represents H-- or HO--.

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