Method for use of branched nucleic acid probes

  • US 5,451,503 A
  • Filed: 06/07/1994
  • Issued: 09/19/1995
  • Est. Priority Date: 01/22/1992
  • Status: Expired due to Term
First Claim
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1. A method for detecting the presence or amount of a target nucleic acid in a sample comprising the steps of:

  • contacting one or more nucleic acid molecules with said sample under a predetermined environmental condition to allow hybridization of said molecules if said target nucleic acid is present;

    wherein said one or more nucleic acid molecules together comprise at least two separate target regions which hybridize with said target nucleic acid under said predetermined environmental condition, and at least two arm regions, wherein said arm regions, under said predetermined environmental condition, do not hybridize with said target nucleic acid, or with each other in the absence of said target nucleic acid, but in the presence of said target nucleic acid do hybridize with each other; and

    detecting any hybridization of said arm regions as an indication of the presence or amount of said target nucleic acid wherein said detecting is not dependent upon interacting labels.

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