Process for the degradation of coal tar and its constituents by Phanerochaete chrysosporium

• US 5,459,065 A
• Filed: 05/21/1993
• Issued: 10/17/1995
• Est. Priority Date: 02/19/1985
• Status: Expired due to Fees

• Current Assignee: Utah Valley University
• Original Assignee: Utah Valley University
• Inventors: Bumpus, John A., Aust, Steven D.
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): Ware, Deborah K.
• Application Number:
  US08/065,563
• Time in Patent Office: 879 Days
• Field of Search: 435/171, 435/254, 435/262.5, 435/, 435/254.1, 210/601, 210/606, 210/610, 210/611, 210/632, 162/1, 162/9, 162/72
• US Class Current: 435/262.5
• CPC Class Codes: A62D 2101/20 Organic substances
• CPC Class Codes: A62D 3/02 by biological methods, i.e. processes using enzymes or microorganisms
• CPC Class Codes: B09C 1/105 using fungi or plants
• CPC Class Codes: C02F 3/34 characterised by the microorganisms used
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12R 2001/645 Fungi ; Processes using fungi

Abstract

A method for degrading coal tar, coal tar distillation fractions, and organic compounds specifically those compounds having three or more fused rings, of the type often associated with coal tar, whether derived from coal tar or synthesized independently. According to the present invention degradation takes place by means of the nonspecific degradation reaction used by Phanerochaete chrysoporium to degrade lignin. The degradation reaction occurs in part by means of a lignin degrading enzyme and hydrogen peroxide, both produced by Phanerochaete chrysoporium.

Claims

1. A process for degrading degradation-resistant organic pollutants comprising the steps of:

a. combining at least one degradation-resistant organic pollutant which is an aromatic hydrocarbon having three or more fused rings selected from the group consisting of coal tar, constituents of coal tar, distillation fractions of coal tar, and polycyclic aromatic organic compounds capable of being derived from coal tar or a constituent or distillation fraction thereof, with lignin-degrading fungal enzymes expressed by Phanerochaete chrysosporium placed in contact with the pollutant, in the presence of hydrogen peroxide; and

b. allowing a degradation reaction to proceed until the pollutant is converted to less toxic degradation products.

2. A process for degrading degradation-resistant organic pollutants as defined in claim 1 wherein the degradation-resistant pollutant comprises an aromatic hydrocarbon having three or more fused rings in creosote.

3. A process for degrading degradation-resistant organic pollutants as defined in claim 1 wherein the degradation-resistant pollutant comprises an aromatic hydrocarbon having three or more fused rings in anthracine oil.

4. A process for degrading degradation-resistant organic pollutants as defined in claim 1 wherein the degradation-resistant pollutant comprises benzo(a)pyrene.

5. A process for degrading degradation-resistant organic pollutants as defined in claim 1 wherein the degradation-resistant pollutant is carcinogenic.

6. A process for degrading degradation-resistant organic pollutants as defined in claim 1 wherein the degradation process occurs under aerobic conditions.

7. A process for degrading degradation-resistant organic pollutants as defined in claim 1 wherein the fungal enzymes comprise at least one lignin-degrading peroxidase.

8. A process for degrading degradation-resistant organic pollutants as defined in claim 1 wherein before step (a) the Phanerochaete chrysosorium has been grown in a growth medium containing a minimal nitrogen source level.

9. A process for degrading degradation-resistant organic pollutants as defined in claim 8 wherein the Phanerochaete chrysosporium is combined with soil containing the pollutant and such that degradation of the pollutant takes place in situ.

10. A process for degrading degradation-resistant organic pollutants as defined in claim 1 wherein nutrients are added during the degradation process.

11. A process for degrading degradation-resistant organic pollutants as defined in claim 10 wherein the nutrients comprise lignin.

12. A process for degrading degradation-resistant organic pollutants as defined in claim 10 wherein the nutrients comprise glucose.

13. A process for degrading degradation-resistant organic pollutants as defined in claim 10 wherein the nutrients comprise corn cobs.

14. A process for degrading degradation-resistant organic pollutants as defined in claim 10 wherein the nutrients comprise peas.

15. A process for degrading degradation-resistant organic pollutants as defined in claim 10 wherein the nutrients comprise straw.

16. A process for degrading degradation-resistant organic pollutants as defined in claim 10 wherein the nutrient is selected from the group consisting of saw dust and wood chips.

17. A process for degrading degradation-resistant organic pollutants as defined in claim 10 wherein nutrient comprises peanut shells.

18. A process for degrading degradation-resistant organic pollutants as defined in claim 10 wherein the nutrients are added periodically during the degradation process.

19. A process for degrading the degradation-resistant organic pollutants as defined in claim 1, further comprising the step of allowing the degradation reaction to proceed until the pollutant is converted to nontoxic degradation products comprising water and carbon dioxide.

20. A process for degrading degradation-resistant organic pollutants as defined in claim 1, wherein the hydrogen peroxide is produced by the Phanerochaete chrysosporium.

21. A process for degrading degradation-resistant organic pollutants as defined in claim 1, wherein the pollutant is converted to nontoxic intermediate degradation products.

22. A process for degrading degradation-resistant organic pollutants as defined in claim 21, wherein the hydrogen peroxide is produced by the Phanerochaete chrysosporium.

23. A process for degrading fused ring aromatic constituents of coal tar which comprises:

a) providing a degradation resistant aromatic constituent of coal tar having three or more fused rings mixed with Phanerochaete chrysosporium expressing a lignin degrading peroxidase in a growth medium containing a minimal nitrogen source level under aerobic conditions; and

b) degrading the fused ring aromatic constituent of coal tar to form less toxic degradation products.

24. A process for degrading fused ring aromatic constituents of coal tar as defined in claim 23 wherein the Phanerochaete chrysosporium has been grown in the growth medium prior to the Phanerochaete chrysosporium being mixed with the fused ring aromatic constituent of coal tar.

25. A process for degrading fused ring aromatic constituents of coal tar as defined in claim 24 wherein the growth medium includes a source of carbon, thiamine and minerals for the Phanerochaete chrysosporium.

26. A process for degrading fused ring aromatic constituents of coal tar as defined in claim 25 wherein the source of carbon is glucose.

27. A process for degrading fused ring aromatic constituents of coal tar as defined in claim 25 wherein the source of carbon is a carbohydrate.

28. A process for degrading fused ring aromatic constituents of coal tar as defined in claim 23 wherein the fused ring aromatic constituent of coal tar is mixed with soil from the environment and wherein the soil is mixed with the Phanerochaete chrysosporium.

29. A process for degrading fused ring aromatic constituents of coal tar as defined in claim 23, further comprising the step of degrading the fused ring aromatic constituent of coal tar to form nontoxic degradation products comprising water and carbon dioxide.