Spectroscopic sample holder and method for using same

• US 5,470,757 A
• Filed: 06/28/1994
• Issued: 11/28/1995
• Est. Priority Date: 06/25/1991
• Status: Expired due to Term

• Current Assignee: 3M Company
• Original Assignee: 3M Company
• Inventors: Gagnon, James E., Povey, Neale P., Midgley, Roland R., Gagnon, David R.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Kim, Christopher Y.
• Application Number:
  US08/267,839
• Time in Patent Office: 518 Days
• Field of Search: 422/58, 422/82.08, 422/82.09, 436/164, 436/169, 436/170, 356/36, 356/38, 356/244
• US Class Current: 436/164
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/3563 for analysing solids; Preparation of samples therefor
• CPC Class Codes: G01N 21/3577 for analysing liquids, e.g. polluted water
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence

Abstract

A sample holder for use in infrared spectrophotometric analysis. The holder comprises a microporous sheet and is particularly useful for analysis of solutions, colloids, small particle solids, flowable solids, solvents, and viscous fluids. The microporous sheet preferably has a low absorbance (high spectral transmittance) in infrared wavelengths. The sample holder is especially useful for the analysis of aqueous based samples. Also, a method for using such sample holders for infrared spectrophotometric analysis.

Claims

1. A method for spectroscopically analyzing a sample, said method consisting essentially of:

a) providing a sample holder consisting essentially of a microporous polymeric sheet, at least a portion of said sheet being an exposed transit area for placement of a sample thereon, said sheet being substantially flat across said transit area and having an average baseline transmittance of at least 1 percent and having a plurality of interconnecting microscopic pores opening through at least one face of said sheet, said pores ranging from about 0.1 to about 50 microns in width in their average characteristic width;

b) applying said sample to said transit area of said sheet;

c) transmitting infrared radiation through said sample and said sheet; and

d) analyzing the radiation transmitted through said sample and said sheet in a spectral region of interest.

2. The method of claim 1 wherein said sample holder further comprises a support member.

3. The method of claim 1 further comprising determining the baseline transmittance of said sample holder prior to applying said sample thereto.

4. The method of claim 1 wherein said sample is a solvent soluble material and further comprising dissolving said sample in solvent prior to applying to said sample holder.

5. The method of claim 1 wherein said sample is applied to said sample holder by filtering a stream containing said sample with said sample holder.

6. The method of claim 1 wherein said sample is in the form of fine particles or powder when applied to said sample holder.

7. The method of claim 1 wherein said sample is a biological fluid.

8. The method of claim 1 wherein:

a) two of said sample holders are positioned, one over another, each disposed horizontally;

b) applying said sample to the top sample holder;

c) applying a suitable solvent to said sample such that said solvent passes through said sample and said top sample holder onto said bottom sample holder; and

then

d) spectroscopically analyzing at least one of (1) the sample fraction on said top sample holder or (2) the sample fraction on said bottom sample holder carried there by said solvent.

9. The method of claim 1 wherein said sheet has an electrostatic charge on opposing sides.

10. The method of claim 1 wherein said sheet further comprises azlactone material.

11. The method of claim 10 wherein said sheet comprises a polymer material to which azlactone has been grafted.

12. The method of claim 1 wherein said sheet comprises a non-woven material.

13. The method of claim 1 wherein said sheet has at least one structured surface.

14. The method of claim 1 wherein said sheet comprises a composite comprising a base sheet and an open mesh.

15. The method of claim 1 wherein said sample is allowed to dry prior to said transmitting infrared radiation.

16. The method of claim 7 further comprising spectroscopically analyzing said sample after said applying said sample to said top sample holder and before said applying a suitable solvent to said sample.

17. The method of claim 1 wherein said sample is a material that readily crystallizes when put on a flat surface and wherein when said sample material is applied to said sheet crystallization is retarded.

18. A method for spectroscopically analyzing a sample, said method consisting essentially of:

a) providing a sample holder consisting essentially of a microporous polymeric sheet, at least a portion of said sheet being an exposed transit area for placement of a sample thereon, said sheet having an average baseline transmittance of at least 1 percent and having a plurality of interconnecting microscopic pores opening through at least one face of said sheet, said pores ranging from about 0.1 to about 50 microns in width in their average characteristic width said sheet having pores opening through only one face thereof;

b) applying said sample to said transit area of said sheet, said sample being applied to said face through which said pores open;

c) transmitting infrared radiation through said sample and said sheet; and

d) analyzing the radiation transmitted through said sample and said sheet in a spectral region of interest.