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Thiol labeling of DNA for attachment to gold surfaces

  • US 5,472,881 A
  • Filed: 03/21/1994
  • Issued: 12/05/1995
  • Est. Priority Date: 11/12/1992
  • Status: Expired due to Fees
First Claim
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1. A method for immobilizing a nucleic acid, containing phosphodiester and base moieties, to a surface of a gold substrate so that the base moieties are exposed and unreacted, comprising the steps of:

  • (a) thiolating the nucleic acid by substituting at least one non-bridging internucleotide oxygen atom of each phosphodiester moiety in the nucleic acid with a sulfur atom; and

    (b) depositing the thiolated nucleic acid on the surface of the gold substrate under conditions such that a covalent bond is formed between each sulfur atom and the gold at the surface of the substrate thereby immobilizing the nucleic acid while leaving the base moieties of the nucleic acid exposed and unreacted.

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