Analyte detection with multilayered bioelectronic conductivity sensors

• US 5,491,097 A
• Filed: 02/28/1994
• Issued: 02/13/1996
• Est. Priority Date: 06/15/1989
• Status: Expired due to Fees

• Current Assignee: Biocircuits Corporation
• Original Assignee: Biocircuits Corporation
• Inventors: Guion, Todd, Shafer, Paul T., Ribi, Hans O.
• Primary Examiner(s): Spiegel, Carol A.
• Application Number:
  US08/203,846
• Time in Patent Office: 715 Days
• Field of Search: 422/82.01, 422/82.02, 422/82.03, 422/82.06, 436/806, 436/501, 436/527, 436/531, 436/518, 435/291, 435/817, 435/7.1, 435/7.5, 435/7.92-7.95, 204/403, 427/2, 427/2.11
• US Class Current: 436/518
• CPC Class Codes: B01J 2219/00734 Lipids
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

Methods are provided for the detection of an analyte in a sample using a bioelectronic sensor comprising a thin surfactant polymeric electrically conducting layer to which members of specific binding pairs are bound. Specific binding of analyte or analyte competitor to the bound specific binding pair member results in a change in the conductivity of the polymer. The resultant change in conductivity is related to the presence of analyte in the sample.

Claims

1. A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising:

an electrically inert substrate;

an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead;

a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula;

space="preserve" listing-type="equation">C.sub.x (C.tbd.C).sub.n C.sub.y L M

wherein;

C intends carbon;

x and y are at least 1 and the sum of x and y is in the range of 4-32;

n is 2;

L is a bond or linking group; and

M is a specific binding member which specifically binds to said analyte;

with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups of said alkadiynes, wherein specific binding of said analyte to said specific binding member causes a change in the conductivity of said electrically conducting surfactant polymer;

insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and

means for connecting said leads to an external circuit;

said method consisting of;

contacting said sample with said bioelectronic sensor;

detecting the change in the conductivity of said electrically conducting polymer; and

relating said change to the presence of said analyte in said sample.

2. The method according to claim 1, wherein said analyte is a microorganism.

3. The method according to claim 1, wherein said specific binding pair member is a ligand and said analyte is a receptor.

4. A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising:

an electrically inert substrate;

an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead;

a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula;

space="preserve" listing-type="equation">C.sub.x (C.tbd.C).sub.n C.sub.y L M

wherein;

C intends carbon;

x and y are at least 1 and the sum of x and y is in the range of 4-32;

n is 2;

L is a bond or linking group; and

M is a first specific binding member which specifically binds to said analyte or an analyte competitor;

with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups or said alkadiynes, wherein specific binding of said analyte or said analyte competitor to said specific binding member causes a change in the conductivity of said electrically conducting surfactant polymer;

insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and

means for connecting said leads to an external circuit;

said method consisting of;

combining said sample with said analyte competitor in an assay medium;

contacting said assay medium with said bioelectronic sensor;

detecting the change in the conductivity of said electrically conducting polymer; and

relating said change to the presence of said analyte in said sample.

5. A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising:

an electrically inert substrate;

an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead;

a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula;

space="preserve" listing-type="equation">C.sub.x (C.tbd.C).sub.n C.sub.y L M

wherein;

C intends carbon;

x and y are at least 1 and the sum of x and y is in the range of 4-32;

n is 2;

L is a bond or linking group; and

M is a first specific binding member which binds to a conjugate, wherein said conjugate comprises a receptor which specifically binds said analyte and a second specific binding member which specifically binds to said first specific binding member;

with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups of said alkadiynes, wherein specific binding of said analyte to said conjugate causes a change in the conductivity of said electrically conducting surfactant polymer;

insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and

means for connecting said leads to an external circuit;

said method consisting of;

combining said sample with said conjugate in an assay medium;

contacting said assay medium with said bioelectronic sensor;

detecting the change in the conductivity of said electrically conducting polymer; and

relating said change to the presence of said analyte in said sample.