Short coherence length, doppler velocimetry system

• US 5,501,226 A
• Filed: 10/19/1994
• Issued: 03/26/1996
• Est. Priority Date: 10/19/1994
• Status: Expired due to Term

• Current Assignee: Carl Zeiss Vision Incorporated (Carl-Zeiss-Stiftung), Humphrey Instruments, Inc.
• Original Assignee: Carl Zeiss Vision Incorporated (Carl-Zeiss-Stiftung)
• Inventors: Petersen, Christopher L., Hellmuth, Thomas
• Primary Examiner(s): Sykes, Angela D.
• Assistant Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US08/325,675
• Time in Patent Office: 524 Days
• Field of Search: 128/691, 128/745, 128/664, 128/665, 128/666, 128/667, 128/661.08, 356/28, 356/28.5
• US Class Current: 600/504
• CPC Class Codes: A61B 3/1233 for measuring blood flow, e.g. at the retina

Abstract

Apparatus for measuring the speed of blood in blood vessels in biological samples, for example, in retinal blood vessels, which apparatus includes: (a) a source of a beam of radiation having a principal wavelength, which radiation is substantially spatially coherent and has a temporal coherence length which is less than 1 picosecond; (b) a beam splitter for splitting the beam into a sample beam and reference beam; (c) optical apparatus for directing the sample beam to an area within the biological sample; (d) a reflector for reflecting the reference beam; (e) a detector for detecting an interference between the sample beam reflected from the area and the reflected reference beam and for generating an interference signal; (f) apparatus for altering an optical path length of the reference beam from the beam splitter to the detector at an alteration velocity; and (g) an analyzer for analyzing the interference signal to determine the speed of the blood in the area from a shift of a central frequency of a frequency spectrum of the interference signal from a frequency determined from the alteration velocity and the principal wavelength.

Claims

1. Apparatus for measuring a profile of speed of blood, wherein the speed is parallel or antiparallel to the direction of a sample beam, the profile being across a blood vessel in a biological sample, in particular, in a retinal blood vessel, which apparatus comprises:

a source of a beam of radiation having a principal wavelength, which beam of radiation is substantially spatially coherent and has a temporal coherence length which is less than 1 picosecond;

means for splitting the beam into the sample beam and a reference beam;

means for reflecting the reference beam;

means for altering an optical path length of the reference beam from the splitting means to a detector means at an alteration velocity;

means for directing the sample beam within the biological sample to pass through the blood vessel;

wherein the detector means comprises means;

(a) for detecting interferences between reflections of the sample beam from matter in the sample including matter situated across the blood vessel and reflections of the reference beam from the reflecting means and (b) for generating an interference signal;

analyzer means for analyzing the interference signal to determine the profile of the speed of the blood from an analysis of frequency spectra of time segments of the interference signal, the alteration velocity in the time segments, and the principal wavelength.

2. The apparatus of claim 1 wherein the analyzer means comprises means for determining, within a time segment, the speed of blood in a portion of the blood vessel from a central frequency of a frequency spectrum of the interference signal in the time segment, the alteration velocity in the time segment, and the principal wavelength.

3. The apparatus of claim 2 wherein the analyzer means further comprises means for determining, within the time segment, the speed of blood in the portion of the blood vessel from a width of the frequency spectrum of the interference signal in the time segment, the alteration velocity, the principal wavelength, and the temporal coherence length.

4. The apparatus of claim 3 wherein the analyzer means further comprises means for identifying the time segment which includes means for detecting maxima of an envelope of the interference signal.

5. The apparatus of claim 4 wherein the means for determining further comprises means for determining the profile of speed of blood from the interference signal generated during a single pass of the sample beam through the blood vessel.

6. The apparatus of claim 4 wherein means for determining further comprises means for determining the profile of speed of blood from interference signals generated during a multiplicity of passes of the sample beam through the blood vessel.

7. The apparatus of claim 1 wherein the source of the beam of radiation is a superluminescent light emitting diode, wherein the beam of radiation is focused through a pinhole aperture.

8. The apparatus of claim 1 wherein the source of the beam of radiation is a superluminescent light emitting diode, wherein the beam of radiation is coupled into a single mode optical fiber.

9. The apparatus of claim 1 wherein the means for reflecting the reference beam comprises at least one reflective surface and the means for altering comprises means for translating the means for reflecting.

10. The apparatus of claim 9 which further comprises means for determining the alteration velocity and for applying the alteration velocity as input to the analyzer means.

11. The apparatus of claim 10 wherein the means for translating outputs a position signal to the means for determining and the means for determining comprises means for differentiating the position signal to determine the alteration velocity.

12. The apparatus of claim 1 wherein the beam is coupled into an optical fiber and at least one portion of a path of travel within the apparatus of the sample beam and the reflections of the sample beam is comprised of an optical fiber and at least one portion of a path of travel within the apparatus of the reference beam is comprised of an optical fiber.

13. The apparatus of claim 12 wherein the means for reflecting comprises means for movement in fixed increments and the means for altering comprises one or more acousto-optic modulators attached to the optical fiber in the path of travel of the reference beam.

14. The apparatus of claim 13 wherein the means for altering further comprises means for translating the means for reflecting.

15. The apparatus of claim 12 wherein the means for reflecting comprises means for movement in fixed increments and the means for altering comprises one or more piezo-electric transducers attached to the optical fiber in the path of travel of the reference beam.

16. The apparatus of claim 15 wherein the means for altering further comprises means for translating the means for reflecting.

17. The apparatus of claim 1 wherein the length of the time segments is proportional to the temporal coherence length divided by the alteration velocity.

18. Method for measuring a profile of absolute value of velocity of blood across a blood vessel in a biological sample, in particular, in a retinal blood vessel, which method comprises the steps of:

generating a sample beam having a principal wavelength from a short coherence length Michelson interferometer having a reference beam whose reference beam path is varied at an alteration velocity;

aiming the sample beam at a wall of the blood vessel;

scanning the sample beam over the blood vessel in a first direction by varying the reference beam path at the alteration velocity and detecting an interference signal produced by an interference between reflections of the sample beam and the reference beam;

determining a first position of a first wall and a first position of a second wall from the interference signal;

determining components of velocity of the blood parallel or antiparallel to a direction of the sample beam from the interference signal by analyzing frequency spectra of time segments of the interference signal;

scanning the sample beam in a second direction to determine a second position of the second wall;

determining an angle of the first direction with respect to a direction of the blood vessel from the positions; and

determining the absolute velocity profile of the blood from the components of velocity and the angle.

19. Method for measuring a profile of absolute value of velocity of blood across a blood vessel in a biological sample, in particular, in a retinal blood vessel, which method comprises the steps of:

generating a sample beam having a principal wavelength from a short coherence length Michelson interferometer having a reference beam whose reference beam path is varied at an alteration velocity;

aiming the sample beam at a wall of the blood vessel;

scanning the sample beam over the blood vessel in a first direction by varying the reference beam path at the alteration velocity and detecting an interference signal produced by an interference between reflections of the sample beam and the reference beam;

determining a first position of a first wall from the interference signal;

determining components of velocity of the blood parallel or antiparallel to a direction of the sample beam and a first position of a maximum of velocity from the interference signal by analyzing frequency spectra of time segments of the interference signal;

scanning the sample beam in a second direction to determine a second position of a maximum of velocity;

determining an angle of the first direction with respect to a direction of the blood vessel from the first position and the second position; and

determining the absolute velocity profile of the blood from the components of velocity and the angle.