Calibration solutions useful for analysis of biological fluids and methods employing same
First Claim
1. A method for the calibration of a sensor employed for the detection of CO2 partial pressure (pCO2), and optionally, O2 partial pressure (pO2), in physiological fluid, said method comprisingcontacting a sensor assembly having:
- a fluid passageway and a sensor for pCO2, and optionally a sensor for pO2, exposed to said fluid passageway, andmeans to pass physiological fluid or calibrant or both over said sensor,with an infusion medium/calibrant comprising;
non-buffered physiological sodium chloride-containing saline, andan amount of a carbon dioxide source effective to provide a stable pCO2 in the range of about 1 up to 100 mm Hg, and optionally a stable pO2 in the range of about 0 up to 200 mm Hg, and thereafter calibrating said sensor.
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Abstract
Novel calibration solutions are provided which are useful, for example, with sensor assemblies used for analysis of CO2, and optionally, for concurrent analysis of O2, especially in combination infusion fluid delivery/blood chemistry analysis systems which include a sensor assembly with each of the assembly electrodes mounted in an electrode cavity in the assembly. The analysis system used in the practice of the present invention typically includes provision for delivering infusion fluid and measuring blood chemistry during reinfusion of the physiological fluid at approximately the same flow rates. The invention calibration solutions are useful for calibrating an array of sensors capable of simultaneously measuring a number of blood chemistry parameters, including the partial pressures (tensions) of carbon dioxide and oxygen, pH (hydrogen ion), sodium, potassium, ionized calcium, ionized magnesium, chloride, glucose, lactate and hematocrit, in body fluids. More particularly, this invention relates to the use of an infusible intravenous solution to facilitate calibration on a regular basis for every individual sensor in a sensor assembly, which is in constant fluid communication with the body regardless of whether the sensors are ex vivo or in vivo.
599 Citations
16 Claims
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1. A method for the calibration of a sensor employed for the detection of CO2 partial pressure (pCO2), and optionally, O2 partial pressure (pO2), in physiological fluid, said method comprising
contacting a sensor assembly having: -
a fluid passageway and a sensor for pCO2, and optionally a sensor for pO2, exposed to said fluid passageway, and means to pass physiological fluid or calibrant or both over said sensor, with an infusion medium/calibrant comprising; non-buffered physiological sodium chloride-containing saline, and an amount of a carbon dioxide source effective to provide a stable pCO2 in the range of about 1 up to 100 mm Hg, and optionally a stable pO2 in the range of about 0 up to 200 mm Hg, and thereafter calibrating said sensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for the assay of CO2 partial pressure (pCO2), and optionally O2 partial pressure (pO2), in physiological fluid, said method comprising
contacting an assembly having: -
a fluid passageway and a sensor for pCO2, and optionally a sensor for pO2, exposed to said fluid passageway, and means to pass physiological fluid or calibrant or both over said sensor, with an infusible calibrant comprising; non-buffered physiological sodium chloride-containing saline, and an amount of a carbon dioxide source effective to provide a stable pCO2 in the range of about 1 up to 100 mm Hg, and optionally a stable pO2 in the range of about 0 up to 200 mm Hg; and
calibrating said pCO2 sensor; and
thereaftercontacting said assembly with said physiological fluid and measuring the pCO2 in said physiological fluid.
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15. In a method for the assay of pCO2, and optionally, pO2, in physiological fluid employing a sensor assembly, the improvement comprising using an infusible calibrant comprising:
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non-buffered physiological sodium chloride-containing saline, and an amount of a carbon dioxide source effective to provide a stable pCO2 in the range of about 1 up to 100 mm Hg, and optionally a stable pO2 in the range of about 0 up to 200 mm Hg.
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16. In a combination infusion delivery system and chemical analysis system having a sensor which determines CO2 partial pressure (pCO2), and optionally, a sensor which determines O2 partial pressure (pO2), in physiological fluid, wherein physiological saline is employed as the infusion medium, the improvement comprising introducing an amount of a carbon dioxide source into said physiological saline effective to provide a stable pCO2 and optionally a stable pO2 to calibrate said CO2 and optionally said O2 sensor.
Specification