Strip electrode including screen printing of a single layer

• US 5,509,410 A
• Filed: 07/27/1994
• Issued: 04/23/1996
• Est. Priority Date: 09/06/1983
• Status: Expired due to Term

• Current Assignee: Medisense Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Medisense Incorporated (Abbott Laboratories Incorporated)
• Inventors: McCann, James M., Higgins, Irving J., Hill, Hugh A. O., Davis, Graham
• Primary Examiner(s): Shay, David M.
• Application Number:
  US08/281,237
• Time in Patent Office: 636 Days
• Field of Search: 204/153.1, 204/153.12, 204/402, 204/403, 204/407, 204/435, 128/633, 128/637, 128/639, 128/642, 128/635
• US Class Current: 600/393
• CPC Class Codes: A61B 2560/0418 Pen-shaped housings
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: A61B 5/6848 Needles
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: G01N 27/4035 Combination of a single ion-sensing electrode and a single reference electrode G01N27/406 and G01N27/413 take precedence
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 29/4914 with deforming of lead or terminal
• CPC Class Codes: Y10T 29/49153 with shaping or forcing terminal into base aperture
• CPC Class Codes: Y10T 29/49169 Assembling electrical component directly to terminal or elongated conductor
• CPC Class Codes: Y10T 29/49224 with coating

Abstract

A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a deposit of an enzyme capable of catalyzing a reaction involving the compound and (preferably) an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing an admixture of enzyme, a conductive material, and a mediator as a single layer onto the substrate.

Claims

1. A method of making a single use disposable electrode strip for attachment to signal readout circuitry of a sensor system to detect a current representative of a compound in a blood sample, the strip comprising:

a) an elongated support having a substantially flat, planar surface, adapted for releasable attachment to said readout circuitry;

b) a first conductor extending along said surface and comprising a conductive element for connection to said readout circuitry;

c) an active electrode on said surface in contact with said first conductor, said active electrode comprising, in a single printed layer on said surface an admixture of an enzyme capable of catalyzing a reaction involving a substrate for said enzyme, a conductive material, and a mediator capable of transferring electrons transferred between said enzyme-catalyzed reaction and said first conductor to create a current representative of the activity of said enzyme and representative of said compound;

d) a second conductor extending along said support, comprising a conductive element for connecting to said readout circuitry; and

e) a reference electrode in contact with said second conductive conductor,

the method comprising;

a) supplying an ink comprising said admixture of said enzyme, said conductive material, and said mediator; and

b) printing said active electrode by applying said ink to said surface over an area so dimensioned and sufficiently close to said reference electrode that said active electrode and said reference electrode present a combined effective area small enough to be completely coverable by a drop of blood.

2. The method of claim 1, wherein said step of printing said active electrode comprises screen printing said ink.

3. The method of claim 1, further comprising the steps of positioning said conductive elements for connection to readout circuitry toward one end of the elongated flat support, and positioning the electrodes remote from said end.

4. The method of claim 1, further comprising the step of configuring said conductive elements so as to allow releasable attachment with a socket on a readout meter.

5. The method of claim 1, further comprising the step of configuring said conductive elements so as to allow releasable attachment to a resilient mounting in said socket.

6. The method of claim 1, further comprising the step of configuring said conductive elements so as to allow releasable mounting with opposed contacts in said mounting.

7. The method of claim 1, further comprising providing, on said elongated support at an end thereof remote from said conductive elements, an area not covered by an electrode to allow for handling of said strip.

8. The method of claim 1, wherein said printing step comprises forming only one active electrode on said elongated support.

9. The method of claim 1 further comprising the step of, prior to supplying the admixture, adsorbing said mediator in said admixture on said conductive material in said admixture.