Method of measuring gas concentrations and microfabricated sensing device for practicing same

• US 5,514,253 A
• Filed: 07/13/1994
• Issued: 05/07/1996
• Est. Priority Date: 07/13/1994
• Status: Expired due to Term

• Current Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: i STAT Corporation (Abbott Laboratories Incorporated)
• Inventors: Widrig, Cindra A., Davis, Graham, Lauks, Imants R., Pierce, Raymond J.
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US08/274,460
• Time in Patent Office: 664 Days
• Field of Search: 204/415, 204/403, 204/431, 204/432, 204/153.17, 204/153.18, 204/153.16, 204/421, 422/68.1, 422/82.03, 422/82.04
• US Class Current: 205/782.5
• CPC Class Codes: G01N 27/404 Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid , e.g. Clark-type oxygen sensors

Abstract

A microfabricated sensing device is disclosed, including methods for using the same for the measurement of the concentration of a gas in a sample. Unique sensor configurations are described which substantially eliminate electrode cross-contamination during operation of the microfabricated sensing devices.

Claims

1. A microfabricated sensing device for measuring a concentration of a dissolved gas in a liquid sample, comprising:

(a) a working electrode (WE) and a counter/reference electrode (CRE) established on the same or separate planar substrates, said WE and CRE being separated by a distance effective to minimize WE contamination by components of said CRE;

(b) an electrolyte layer (EL) established on said substrate and enclosing said WE; and

(c) a gas permeable membrane (GPM) established on but not enclosing said EL and positioned over said WE, each of said GPM and EL having respective dimensions and configurations relative to said WE such that a flux of gas to said WE, from a liquid sample in contact with the device, is a function of an amount of gas passing through said GPM.

2. The device of claim 1 in which said distance is at least about 1 mm.

3. The device of claim 1 in which said WE comprises gold, said CRE comprises silver/silver chloride, said EL comprises a photoformed gelatin, and said GPM comprises a silicone block copolymer.

4. A microfabricated sensing device for measuring a concentration of a dissolved gas in a liquid sample, comprising:

(a) a planar substrate;

(b) a working electrode (WE) and a counter/reference electrode (CRE) established on said substrate and separated by a distance effective to minimize WE contamination by components of said CRE;

(c) an electrolyte layer (EL) established on said substrate and enclosing said WE; and

(d) a gas permeable membrane (GPM) established on but not enclosing said EL and positioned over said WE, each of said GPM and EL having respective dimensions and configurations relative to said WE such that a flux of gas to said WE, from a liquid sample in contact with the device, is a function of an amount of gas passing through said GPM.

5. The device of claim 4 in which said distance is at least about 1 mm.

6. The device of claim 4 in which said WE comprises gold, said CRE comprises silver/silver chloride, said EL comprises a photoformed gelatin, and said GPM comprises a silicone block copolymer.

7. A method of measuring a concentration of a dissolved gas in a liquid sample, comprising the steps of:

(a) providing a microfabricated sensing device comprising (i) a working electrode (WE) and a counter/reference electrode (CRE) established on the same or separate planar substrates, said WE and CRE being separated by a distance effective to minimize WE contamination by components of said CRE, (ii) an electrolyte layer (EL) established on said substrate and enclosing said WE, and (iii) a gas permeable membrane (GPM) established on but not enclosing said EL and positioned over said WE, each of said GPM and EL having respective dimensions and configurations relative to said WE such that a flux of gas to said WE, from a liquid sample in contact with the device, is a function of an amount of gas passing through said GPM;

(b) contacting a liquid sample containing a gas to said device such that said liquid sample provides a low impedance path between said WE and CRE;

(c) applying a potential to said WE with respect to said CRE effective to allow molecules of the gas to undergo a redox reaction at said WE;

(d) measuring a current output of said device; and

(e) determining a concentration of the gas based on said measured current.

8. The method of claim 7 in which said gas is oxygen.

9. A method of measuring a concentration of a dissolved gas in a liquid sample, comprising the steps of:

(a) providing a microfabricated sensing device comprising (i) a working electrode (WE) and a reference electrode RE established on the same or separate planar substrates, said WE and RE being separated by a distance effective to minimize WE contamination by components of said RE, (ii) an electrolyte layer (EL) established on said substrate and enclosing said WE, and (iii) a gas permeable membrane (GPM) established on but not enclosing said EL and positioned over said WE, each of said GPM and EL having respective dimensions and configurations relative to said WE such that a flux of gas to said WE, from a liquid sample in contact with the device, is a function of an amount of gas passing through said GPM;

(b) contacting a liquid sample containing a gas to said device such that said liquid sample provides a low impedance path between said WE and RE;

(c) measuring a potential between said WE and RE; and

(d) relating said potential to a concentration of the gas in the liquid sample.

10. The method of claim 9 in which said gas is carbon dioxide.

11. A microfabricated sensing device for measuring a concentration of a gas in a sample, comprising:

(a) a working electrode (WE) and a counter/reference electrode (CRE) established on the same substrate, said WE and CRE being separated by a distance effective to minimize WE contamination by components of said CRE;

(b) an electrolyte layer (EL) established on said substrate and enclosing said WE;

(c) a conducting layer (CL) established on said substrate to provide electrical contact between said WE and said CRE; and

(d) a gas permeable membrane (GPM) established on but not enclosing said EL and positioned over said WE, each of said GPM and EL having respective dimensions and configurations relative to said WE such that the flux of gas to said WE, from a sample in contact with the device, is a function of an amount of the gas passing through said GPM.

12. The device of claim 11 in which said EL is in electrical contact with said CRE, thus serving as said CL.

13. The device of claim 11 in which said sample is selected from the group consisting of air, a gas, wet air, a wet gas or liquid.

14. The device of claim 11 in which said distance is at least about 1 mm.

15. The device of claim 11 in which said WE comprises gold, said CRE comprises silver/silver chloride, said EL comprises a photoformed gelatin, and said GPM comprises a silicone block copolymer.

16. The device of claim 11 is which said EL comprises a photoformed gelatin whose permeability to gases has been reduced by an increased ratio of crosslinker to gelatin solids.

17. The device of claim 11 in which said EL comprises a photoformed gelatin whose permeability to gases has been reduced by an extended bake step at elevated temperature.

18. The device of claim 17 in which said photoformed gelatin had been baked at 120°

C. for one hour.

19. The device of claim 11 in which said CL comprises a photoformed gelatin layer containing hygrocopic salts.

20. The device of claim 11 in which said CL comprises a conducting polymer.

21. The device of claim 20 in which said conducting polymer is selected from the group consisting of polypyrrole, polythiophene, and polyaniline.

22. The device of claim 21 in which said conducting polymer further comprises a dopant.

23. The device of claim 21 in which said conducting polymer further comprises a dopant selected from the group consisting of halides, nitrates, phosphates, and sulfates.

24. The device of claim 20 in which said conducting polymer further comprises a dopant selected from the group consisting of halides, nitrates, phosphates, and sulfates.

25. The device of claim 20 in which said conducting polymer further comprises a dopant.

26. A method of measuring a concentration of a gas in a sample, comprising the steps of:

(a) providing a microfabricated sensing device comprising (i) a working electrode (WE) and a counter/reference electrode (CRE) established on the same substrate, said WE and CRE being separated by a distance effective to minimize WE contamination by components of said CRE, (ii) an electrolyte layer (EL) established on said substrate and enclosing said WE, (iii) a conducting layer (CL) established on said substrate to provide electrical contact between said WE and said CRE, and (iv) a gas permeable membrane (GPM) established on but not enclosing said EL and positioned over said WE, each of said GPM and EL having respective dimensions and configurations relative to said WE such that a flux of gas to said WE, from a sample in contact with the device, is a function of an amount of gas passing through said GPM;

(b) contacting a sample containing a gas to said device;

(c) applying a potential to said WE with respect to said RE effective to allow molecules of the gas to undergo a redox reaction at said WE;

(d) measuring a current output of said device; and

(e) determining a concentration of the gas based on said current output.

27. The method of claim 26 in which the concentration of the gas is determined by comparing the measured current output to a standard curve relating current output to a partial pressure of a gas in a sample.

28. The method of claim 26 which further comprises contacting said microfabricated sensing device with a calibrating fluid before or after the device is contacted with a sample.

29. A method of measuring a concentration of a gas in a sample, comprising the steps of:

(a) providing a microfabricated sensing device comprising (i) a working electrode (WE) and a reference electrode (RE) established on the same substrate, said WE and RE being separated by a distance effective to minimize WE contamination by components of said RE, (ii) an electrolyte layer (EL) established on said substrate and enclosing said WE, (iii) a conducting layer (CL) established on said substrate to provide electrical contact between said WE and said RE, and (iv) a gas permeable membrane (GPM) established on but not enclosing said EL and positioned over said WE, each of said GPM and EL having respective dimensions and configurations relative to said WE such that a flux of gas to said WE, from a sample in contact with the device, is a function of an amount of gas passing through said GPM;

(b) contacting a sample containing a gas to said device;

(c) measuring a potential between said WE and RE; and

(d) determining a concentration of the gas based on said measured potential.

30. The method of claim 29 in which the concentration of the gas is determined by comparing the measured potential to a standard curve relating potential to a partial pressure of a gas in a sample.

31. The method of claim 29 which further comprises contacting said microfabricated sensing device with a calibrating fluid before or after the device is contacted with a sample.

32. A microfabricated sensing device for measuring a concentration of an analyte in a liquid sample, comprising:

(a) a working electrode (WE) and a counter/reference electrode (CRE) established on the same planar substrate, said WE and CRE being substantially different in at least one dimension;

(b) at least a first layer established over said WE, said WE and CRE being separated by a distance effective to minimize adverse effects of said substantially different at least one dimension on topological features of said first layer.

33. The device of claim 32 in which said analyte is a dissolved gas.

34. The device of claim 32 in which said first layer comprises an electrolyte layer.

35. The device of claim 34, further comprising a second layer established over said first layer.

36. The device of claim 35 in which said second layer comprises a gas permeable layer positioned over said WE but which does not enclose said first layer.

37. The device of claim 32 in which said dimension is the thickness of said electrodes.

38. The device of claim 32 in which said distance is at least about 1 mm.