Method for detecting an analyte using an electrochemical luminescent transition metal label

• US 5,541,113 A
• Filed: 09/22/1993
• Issued: 07/30/1996
• Est. Priority Date: 09/22/1993
• Status: Expired due to Fees

• Current Assignee: Beckman Instruments, Inc. (Danaher Corporation)
• Original Assignee: Beckman Instruments, Inc. (Danaher Corporation)
• Inventors: Siddigi, Iqbal W., Sternberg, James C.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): CARRILLO, BIBI SHARIDAN
• Application Number:
  US08/125,437
• Time in Patent Office: 1,042 Days
• Field of Search: 436/518, 436/512, 436/519, 436/525, 436/536, 436/557, 436/544, 436/546, 436/548, 436/904, 436/905, 436/56, 436/172, 436/164, 435/4, 435/5, 435/6, 435/7.1, 435/50, 422/52, 422/82.05, 422/82.08, 422/82.06, 546/2, 250/361 C
• US Class Current: 436/56
• CPC Class Codes: G01N 21/69 specially adapted for fluids , e.g. molten metal
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: Y10T 436/13 Tracers or tags

Abstract

A method for detecting an analyte, in an aqueous solution at a physiological pH, by reductive or oxidative/reductive electrochemical luminescence methodologies is disclosed. The method proceeds by labelling the analyte with a transition metal complex, followed by inducing the transition metal label to luminescence by application of a suitable electrical potential to a solution containing the label and the analyte. The transition metal complex can be a tris-ruthenium(bipyridine) complex. A hydroxylamine and/or a halogen-containing moiety can be used to enhance both reductive and/or oxidative electrochemical luminescence of the transition metal complex. The transition metal chelate can be used as a label for the detection of picomolar concentrations of an analyte of interest, such as an analyte present in a sample of a physiological fluid.

Claims

1. A method for detecting an analyte, comprising the steps of:

a) adding a label, an hydroxylamine, and an analyte of an aqueous solution;

b) stimulating the label to emit electromagnetic radiation in an electrochemical luminescence reaction, in the absence of applying an oxidative potential by applying a reducing potential to the aqueous solution, and;

(c) detecting the analyte by detecting the electromagnetic radiation emitted by the label.

2. The method of claim 1, further comprising the step of quantifying the amount of the analyte.

3. The method of claim 1, wherein the analyte is added to the aqueous solution in a sample of a physiological fluid.

4. The method of claim 1, wherein the aqueous solution has a physiological pH.

5. The method of claim 1, wherein the aqueous solution has a pH between about 6 and about 8.

6. The method of claim 1, wherein the aqueous solution has a physiological pH that is maintained by addition of a suitable buffer to the aqueous solution prior to the adding step.

7. The method of claim 1, wherein the aqueous solution is at least about 95% by weight water.

8. The method of claim 1, wherein the aqueous solution is at least about 98% by volume water.

9. The method of claim 1, wherein the label comprises a transition metal.

10. The method of claim 1, wherein the label comprises an organic ligand.

11. The method of claim 9 wherein the transition metal is selected from the group consisting of chromium, ruthenium, rhodium, rhenium, osmium and iridium.

12. The method of claim 1, wherein the electromagnetic radiation is emitted as light in the visible spectrum.

13. The method of claim 1, wherein the electromagnetic radiation emitted has an emission maximum at a wavelength between about 200 nm and about 900 nm.

14. The method of claim 1, Wherein the reducing potential applied to the aqueous solution is at least about -600 millivolts.

15. The method of claim 1, wherein the method further comprises the step of adding an amine to the aqueous solution prior to the stimulating step.

16. The method of claim 15, wherein the amine is a tri-n-propylamine.

17. The method of claim 1, wherein the hydroxylamine is selected from the group consisting of hydroxylamine, N-cyclohexyl-hydroxylamine, N-tertbutyl-hydroxylamine, N-methylhydroxylamine, and N-isopropyl-hydroxylamine.

18. The method of claim 1 further including a halide ion.

19. The method of claim 1, further comprising the step of applying a positive potential to the aqueous solution prior to application of the reducing potential to the aqueous solution.

20. The method of claim 19 wherein the positive potential applied to the aqueous solution is an oxidative potential.

21. The method of claim 20, wherein the oxidative potential applied to the aqueous solution is at least about +800 millivolts.

22. A method for detecting an analyte, comprising the steps of:

(a) preparing an aqueous solution comprising a suitable buffer for maintaining the aqueous solution at a physiological pH;

(b) selecting a label comprising a transition metal and an organic ligand chelated to the transition metal, the label being capable of emitting electromagnetic radiation with an emission maximum between about 200 nm and about 900 nm in an electrochemical luminescence reaction;

(c) adding;

(i) an analyte present in a sample of a physiological fluid,

(ii) the label,

(iii) an amine and,

(iv) an ECL facilitator selected from the group consisting of halides and hydroxylamines, to the aqueous solution, the aqueous solution thereby comprising at least about 95% by weight water and at least about 98% by volume water;

(d) stimulating the label to emit electromagnetic radiation in an electrochemical luminescence reaction, in the absence of applying an oxidative potential by applying a reducing potential to the aqueous solution,

(e) detecting the presence or the absence of the analyte by detecting the electromagnetic radiation; and

(f) quantifying the amount of analyte present in the sample of the physiological fluid.

23. The method of claim 22, wherein the amine comprises a tri-n-propylamine.

24. The method of claim 22, wherein the ECL facilitator comprises a halide.

25. The method of claim 22, wherein the electric potential applied to the aqueous solution is an oxidative potential.

26. The method of claim 22, wherein the oxidative potential applied to the aqueous solution is less than about +1 volt.

27. The method of claim 22, wherein the oxidative potential applied to the aqueous solution is less than about +0.9 volts.

28. The method of claim 22, wherein the oxidative potential applied to the aqueous solution is between about +1.3 volts and about +0.7 volts.

29. The method of claim 22, wherein the oxidative potential applied to the aqueous solution is between about +1.3 volts and about +0.9 volts.

30. The method of claim 22, wherein the oxidative potential applied to the aqueous solution is between about +1 volt and about +0.9 volts.

31. The method of claim 22, wherein the electric potential is a reducing potential.

32. The method of claim 22, wherein a reducing potential is applied to the aqueous solution subsequent to application of the oxidative potential.

33. The method of claim 22, wherein less than about 10 pM of the analyte can be detected.

34. The method of claim 22, wherein the stimulating step comprises the step of maintaining the electric potential at a substantially constant voltage for at time period of between about two and about thirty seconds to enhance the emission of the electromagnetic radiation by the label.

35. The method of claim 34 wherein the electric potential is maintained for about five seconds.

36. A method for detecting a transition metal complex, comprising the steps of:

(a) adding a transition metal complex and an hydroxylamine to an aqueous solution;

(b) stimulating the transition metal complex to emit electromagnetic radiation in an electrochemical luminescence reaction, in the absence of applying an oxidation potential by applying a reducing potential to the aqueous solution, and;

(c) detecting the transition metal complex by detecting the electromagnetic radiation emitted by the transition metal complex.