Detection of an analyte by fluorescence using a thin film optical device

• US 5,552,272 A
• Filed: 06/10/1993
• Issued: 09/03/1996
• Est. Priority Date: 06/10/1993
• Status: Expired due to Term

• Current Assignee: Inverness Medical - Biostar Incorporated
• Original Assignee: BioStar, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Bogart, Gregory R.
• Primary Examiner(s): Saunders, David
• Assistant Examiner(s): Chin, Christopher L.
• Application Number:
  US08/076,348
• Time in Patent Office: 1,181 Days
• Field of Search: 422/55, 422/57, 422/58, 422/82.05, 422/82.08, 422/82.11, 435/6, 435/7.2, 435/7.3, 435/7.32, 435/7.72, 435/808, 435/810, 436/172, 436/518, 436/524, 436/525, 436/527, 436/528, 436/531, 436/805, 436/807, 359/580, 359/585
• US Class Current: 435/6.12
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures

Abstract

Device for detecting the presence or amount of an analyte of interest, comprising a reflective solid, optical support and a label capable of generating fluorescent signal upon excitation with a suitable light source wherein said support comprises an attachment layer comprising a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes wherein the support provides an enhanced level of exciting photons to the immobilized fluorescent label compound, and wherein the support also increases the capture of fluorescent signal.

Claims

1. Assay system for detecting the presence or amount of an analyte of interest, comprising:

a reflective solid, optical substrate;

an attachment layer provided on an uppermost surface of said substrate, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

a receptive layer provided on an uppermost surface of said attachment layer comprising a specific binding partner for said analyte;

a label capable of generating fluorescent signal upon excitation with a suitable light source attached to a specific binding reagent so as to allow said label to become bound to said analyte;

wherein said substrate provides an enhanced level of exciting photons directly to said label when said label is bound to said receptive layer in the presence of said analyte and said substrate also increases the capture of fluorescent signal relative to capture in the absence of said substrate;

and wherein capture of said signal by a detector indicates the presence or amount of said analyte of interest.

9. The assay system of any of claims 1-8 wherein the receptive layer is an antibody.

10. The assay system of any of claims 1-8, wherein the receptive layer is a nucleic acid.

11. The assay system of any of claims 1-8, wherein the receptive layer is formed from material selected from the group consisting of oligonucleotides, chelators, enzymes, bacteria, bacterial pili, bacterial flagellar materials, nucleic acids, polysaccharides, lipids, carbohydrates, metals, viruses, hormones, and receptors for said materials.

12. The assay system of any of claims 1-8, wherein said analyte is selected from the group consisting of an antibody, enzyme, hormone, polysaccharides, lipids, carbohydrates, and nucleic acids.

13. The assay system of any of claims 1-8, wherein the receptive layer is an antigen.

14. The assay system of any of claims 1-8, wherein said analyte is an antigen.

15. The assay system of any of claims 1-8, wherein said analyte is a drug.

16. The assay system of any of the claims 1-8, wherein said analyte is protein.

17. The assay system of any of claims 1-8, wherein the detector and the light source are positioned at equal angles relative to the substrate.

18. The assay system of any of claims 1-8, wherein the light source is monochromatic or polychromatic.

20. The assay system of any of claims 1-8, wherein the detector is positioned at any angle relative to the substrate.

2. Assay system for detecting the presence or amount of an analyte of interest, comprising:

a reflective solid, optical substrate;

an attachment layer provided on an uppermost surface of said substrate, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

a receptive layer provided on an uppermost surface of said attachment layer comprising a specific binding partner for said analyte and for a specific binding reagent attached to a label;

said label capable of generating fluorescent signal upon excitation with a suitable light and able to bind to said receptive layer by said specific binding reagent;

wherein aid substrate provided an enhanced level of exciting photons directly to said label when said lable is bound to said receptive layer and said receptive layer and said substrate also increases the capture of fluorescent signal relative to capture in the absence of said substrate;

and wherein capture of said signal by a detector indicates the presence or amount of said analyte of interest which competes with said label for binding to said receptive layer.

3. Assay system for detecting the present or amount of an analyte of interest, comprising:

a reflective solid, optical substrate;

an anti-reflective film provided on an uppermost layer of said substrate comprising a material selected from the group consisting of silicon nitride, silicon/silicon dioxide composites, silicon oxynitride, titanium dioxide, titanates, diamond, oxides of zirconium, and silicon carbide;

and wherein the film is selected to anti-reflect an exciting light;

an attachment layer provided on an uppermost surface of said anti-reflective film, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

a receptive layer provided on an uppermost surface of said attachment layer comprising a specific binding partner for said analyte;

a label capable of generating fluorescent signal upon excitation with a suitable light source attached to a specific binding reagent so as to allow said label to become bound to said analyte;

wherein said substrate provides an enhanced level of exciting photons directly to said label when said label is bound to said receptive layer in the presence of said analyte and said substrate also increases the capture of fluorescent signal relative to capture in the absence of said substrate;

and wherein capture of said signal by a detector indicates the presence of amount of said analyte of interest.

19. The assay system of any of claims 3-6, wherein said AR film is selected to extinguish the exciting wavelength specific to the fluorophore.

4. Assay system for detecting the presence or amount of an analyte of interest, comprising:

a reflective solid, optical substrate;

an anti-reflective film provided on an uppermost layer of said substrate comprising a material selected from the group consisting of silicon nitride, silicon/silicon dioxide composites, silicon oxynitride, titanium dioxide, titanates, diamond, oxides of zirconium, and silicon carbide;

and wherein the film is selected to anti-reflect an exciting light;

an attachment layer provided on an uppermost surface of said anti-reflective film, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

a receptive layer provided on an uppermost surface of said attachment layer comprising a specific binding partner for said analyte and for a specific binding reagent attached to a label;

said label capable of generating fluorescent signal upon excitation with a suitable light source and able to bind to said receptive layer by said specific binding reagent;

wherein said substrate provides an enhanced level of exciting photons directly to said label when said label is bound to said receptive layer and said substrate also increases the capture of fluorescent signal relative to capture in the absence of said substrate;

and wherein capture of said signal by a detector indicates the presence of amount of said analyte of interest which competes with said label for binding to said receptive layer.

5. Assay system for detecting the presence or amount of an analyte of interest, comprising:

a reflective solid, optical substrate selected from the group consisting of monocrystalline silicon, glass, a glass/amorphous silicon composite, a metal, a ceramic, a plastic, polycrystalline silicon, and composites of these materials;

an anti-reflective film provided on an uppermost layer of said substrate comprising a material selected from the group consisting of silicon nitride, silicon/silicon dioxide composites, silicon oxynitride, titanium dioxide, titanates, diamond, oxides of zirconium, and silicon carbide;

and wherein the film is selected to anti-reflect an exciting light;

an attachment layer provided on an uppermost surface of said anti-reflective film, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

a receptive layer provided on an uppermost surface of said attachment layer comprising a specific binding partner for said analyte;

a label capable of generating fluorescent signal upon excitation with a suitable light source attached to a specific biding reagent so as to allow said label to become bound to said analyte;

wherein said substrate provides an enhanced level of exciting photons directly to said label when said label is bound to said receptive layer in the presence of said analyte and said substrate also increases the capture of fluorescent signal relative to capture in the absence of said substrate;

and wherein capture of said signal by a detector indicates the presence of amount of said analyte of interest.

6. Assay system for detecting the presence or amount of an analyte of interest, comprising:

a reflective solid, optical substrate selected from the group consisting of monocrystalline silicon, glass, a glass/amorphous silicon composite, a metal, a ceramic, a plastic, polycrystalline silicon, and composites of these materials;

an anti-reflective film provided on an uppermost layer of said substrate comprising a material selected from the group consisting of silicon nitride, silicon/silicon dioxide composites, silicon oxynitride, titanium dioxide, titanates, diamond, oxides of zirconium, and silicon carbide;

and wherein the film is selected to anti-reflect an exciting light;

an attachment layer provided on an uppermost surface of said anti-reflective film, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

a receptive layer provided on an uppermost surface of said attachment layer comprising a specific binding partner for said analyte and for a specific binding reagent attached to a label;

said label capable of generating fluorescent signal upon excitation with a suitable light source and able to bind to said receptive layer by said specific binding reagent;

wherein said substrate provides an enhanced level of exciting photons directly to said label when said label is bound to said receptive layer and said substrate also increases the capture of fluorescent signal relative to capture in the absence of said substrate;

and wherein capture of said signal by a detector indicates the presence of amount of said analyte of interest which competes with said label for binding to said receptive layer.

7. Assay system for detecting the presence or amount of an analyte of interest, comprising:

a reflective solid, optical substrate selected from the group consisting of monocrystalline silicon, glass, a glass/amorphous silicon composite, a metal, a ceramic, a plastic, polycrystalline silicon, and composites of these materials;

an attachment layer provided on an uppermost surface of said substrate, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

a receptive layer provided on an uppermost surface of said attachment layer comprising a specific binding partner for said analyte;

a label capable of generating fluorescent signal upon excitation with a suitable light source attached to a specific binding reagent so as to allow said label to become bound to said analyte;

wherein said substrate provides an enhanced level of exciting photons directly to said label when said label is bound to said receptive layer and said substrate also increases the capture of fluorescent signal relative to capture in the absence of said substrate;

and wherein capture of said signal by a detector indicates the presence of amount of said analyte of interest.

8. Assay system for detecting the presence or amount of an analyte of interest, comprising:

a reflective solid, optical substrate selected from the group consisting of monocrystalline silicon, glass, a glass/amorphous silicon composite, a metal, a ceramic, a plastic, polycrystalline silicon, and composites of these materials;

an attachment layer provided on an uppermost surface of said substrate, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

a receptive layer provided on an uppermost surface of said attachment layer comprising a specific binding partner for said analyte and for a specific binding reagent attached to a label;

said label capable of generating fluorescent signal upon excitation with a suitable light source and able to bind to said receptive layer by said specific binding reagent;

wherein said substrate provides an enhanced level of exciting photons directly to said label when said label is bound to said receptive layer and said substrate also increases the capture of fluorescent signal relative to capture in the absence of said substrate;

and wherein capture of said signal by a detector indicates the presence of amount of said analyte of interest which competes with said label for binding to said receptive layer.

21. Method for detecting the presence or amount of an analyte of interest in a sample, comprising the steps of:

providing a reflective solid, optical substrate;

providing an attachment layer on an uppermost surface of said substrate, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

providing on an uppermost layer of said attachment layer a receptive layer, said layer comprising a specific binding partner for said analyte;

providing a label capable of generating fluorescent signal upon excitation with a suitable light source, said label attached to a specific binding reagent so as to allow said label to become affixed to said receptive layer by binding to said analyte;

contacting said substrate with a sample potentially comprising said analyte of interest under conditions in which said analyte can interact with said receptive layer on said substrate;

contacting said bound analyte with said label so as to allow said substrate to provide an enhanced level of excitation to said label so as to generate a fluorescent signal and enhance the capture of said signal;

and capturing said signal, as an indicator of the presence or amount of said analyte, with a detector.

24. The method of claim 21, 22, or 23 wherein said substrate further comprises an AR film comprising a material selected from the group consisting of silicon nitride, silicon/silicon dioxide composites, silicon oxynitride, titanium dioxide, titanates, diamond, oxides of zirconium, and silicon carbide, and said AR film is selected to extinguish the exciting wavelength specific to the fluorophore.

25. The method of claim 21, 22, or 23 wherein the light source and the detector are positioned at equal angles relative to the substrate.

26. The method of claim 21, 22, or 23 wherein the fluorescent label is conjugated to a nucleic acid probe.

27. The method of claim 21, 22, or 23 wherein said optical substrate is selected from the group consisting of monocrystalline silicon, glass, a glass/amorphous silicon composite, a metal, a ceramic, a plastic, polycrystalline silicon, and composites of these materials.

22. Method for detecting the presence or amount of an analyte of interest in a sample, comprising the steps of:

providing a reflective solid, optical substrate;

providing an attachment layer on an uppermost surface of said substrate, wherein said attachment layer comprise a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

providing on an uppermost layer of said attachment layer a receptive layer, said layer comprising a specific binding partner for said analyte;

providing a label capable of generating fluorescent signal upon excitation with a suitable light source, said label attached to a specific binding reagent so as to allow said label to become affixed to said receptive layer by binding to said analyte;

forming a mixture by contacting said label attached to a specific binding reagent with a sample potentially comprising said analyte of interest under conditions in which said analyte can interact with said specific binding agent attached to said label;

contacting said substrate with said mixture under conditions in which said analyte can interact with said receptive layer on said substrate;

allowing said substrate to provide an enhanced level of excitation to said label so as to generate a fluorescent signal and enhance the capture of said signal;

and capturing said signal, as an indicator of the presence or amount of said analyte, with a detector.

23. Method for detecting the presence or amount of an analyte of interest in a sample, comprising the steps of:

providing a reflective solid, optical substrate;

providing an attachment layer on an uppermost surface of said substrate, wherein said attachment layer comprises a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes;

providing on an uppermost layer of said attachment layer a receptive layer, said layer comprising a specific binding partner for said analyte and for a specific binding reagent attached to a label;

providing a label capable of generating fluorescent signal upon excitation with a suitable light source, said label attached to a specific binding reagent so as to allow said label to become affixed to said receptive layer;

contacting said substrate with said label and a sample potentially comprising said analyte of interest under conditions in which both said analyte and said label can compete for binding with said receptive layer;

whereas said substrate provides an enhanced level of excitation to said label bound to said receptive layer so as to generate a fluorescent signal and enhance the capture of said signal;

and capturing said signal, as an indicator of the presence or amount of said analyte, with a detector.