Assay system
DCFirst Claim
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1. An apparatus comprising, in combination:
- focussing optical lens means;
a conduit means of substantially uniform cross-sectional dimension disposed within said lens means for fluid flow of a fluid sample therethrough, and extending transversely to an optical axis of said lens means through a focal region of said lens means; and
a porous mass of light-transparent material disposed in said conduit means, the porosity of said mass being selected to permit fluid flow of said fluid sample therethrough, said apparatus being arranged and constructed such that said lens means focuses light rays that emanate from said porous mass within said conduit means, said lens means focussing said light rays by refraction.
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Abstract
A system for assaying a fluid sample, typically employing a fluorescent tag, the system comprising a lens capable of focussing both excitation and fluorescent radiation, a fluid-flow conducting conduit being provided in the lens extending transversely of the optical axis of and through the focal region of the latter. One or more mechanical screens are disposed adjacent to the focal region in the conduit to arrest passage of beads as a function of bead diameter. The beads, precoated with at least a moiety of a ligand/conjugate complex, e.g. a specific-binding ligand, are preferably substantially transparent to both the excitation and fluorescent radiation.
33 Citations
14 Claims
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1. An apparatus comprising, in combination:
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focussing optical lens means; a conduit means of substantially uniform cross-sectional dimension disposed within said lens means for fluid flow of a fluid sample therethrough, and extending transversely to an optical axis of said lens means through a focal region of said lens means; and a porous mass of light-transparent material disposed in said conduit means, the porosity of said mass being selected to permit fluid flow of said fluid sample therethrough, said apparatus being arranged and constructed such that said lens means focuses light rays that emanate from said porous mass within said conduit means, said lens means focussing said light rays by refraction. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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2. An apparatus comprising, in combination:
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focussing optical lens means; a conduit means of substantially uniform cross-sectional dimension disposed within said lens means for fluid flow of a fluid sample therethrough, and extending transversely to an optical axis of said lens means through a focal region of said lens means; and a porous mass of light-transparent material disposed in said conduit means, the porosity of said mass being selected to permit fluid flow of said fluid sample therethrough, said apparatus being arranged and constructed such that said lens means focuses light rays that emanate from within said conduit means as said light rays exit said lens means.
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Specification