Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems

  • US 5,556,750 A
  • Filed: 11/01/1993
  • Issued: 09/17/1996
  • Est. Priority Date: 05/12/1989
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for eliminating DNA molecules containing one or more base pairing mismatches from a population of heterohybrid duplex DNA molecules formed by base pairing of single-stranded DNA molecules obtained from a first source and a second source, comprising the steps of:

  • digesting genomic DNA from said first and said second source with a restriction endonuclease,methylating the DNA from one of said sources,denaturing said DNA from said first and said second source,mixing DNA from said first and said second source in the presence of a recombinase protein, proteins of a mismatch repair system that modulate said recombinase protein, single-strand binding protein, and ATP, such that DNA duplexes form in homologous regions of DNA molecules from said first and said second source and the presence of a base pair mismatch results in regions that remain single-stranded, andremoving molecules that contain a said single-stranded region from said population.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×