Polymerase compositions and uses thereof

  • US 5,556,772 A
  • Filed: 02/16/1994
  • Issued: 09/17/1996
  • Est. Priority Date: 12/08/1993
  • Status: Expired due to Term
First Claim
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1. A kit for the synthesis of a polynucleotide, said kit comprising:

  • (a) a first DNA polymerase, wherein said first polymerase possesses 3'"'"'-5'"'"' exonuclease activity selected from the group consisting of Pyrococcus furiosus DNA polymerase, Thermotoga maritima DNA polymerase, Thermococcus litoralis DNA polymerase, and Pyrococcus GB-D DNA polymerase, and(b) a second DNA polymerase, wherein said second polymerase lacks 3'"'"'-5'"'"' exonuclease activity selected from the group consisting of Thermus aquaticus DNA polymerase, (exo-) Thermococcus litoralis DNA polymerase, (exo-) Pyrococcus furiosus DNA polymerase, and (exo-) Pyrococcus GB-D DNA polymerase.

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