Nude mouse model for human neoplastic disease

• US 5,569,812 A
• Filed: 06/02/1995
• Issued: 10/29/1996
• Est. Priority Date: 10/05/1988
• Status: Expired

• Current Assignee: Anticancer Incorporated
• Original Assignee: Anticancer Incorporated
• Inventors: Fu, Xinyu, Monosov, Ann
• Primary Examiner(s): Chambers, Jasemine C.
• Application Number:
  US08/459,730
• Time in Patent Office: 515 Days
• Field of Search: 800/2, 800/DIG. 5, 424/573, 424/574, 424/559, 424/551, 424/556, 424/557, 424/9.2
• US Class Current: 800/10
• CPC Class Codes: A01K 67/0271 Chimeric vertebrates, e.g. comprising exogenous cells
• CPC Class Codes: C12N 5/0693 Tumour cells; Cancer cells

Abstract

A nude mouse model for human neoplastic disease having histologically intact human neoplastic tissue transplanted onto an organ of the mouse which corresponds to the human organ from which the tissue is obtained.

Claims

1. A nude mouse model for human neoplastic disease, wherein said mouse is characterized by:

having histologically intact human neoplastic tissue of at least 1 mm³ in size transplanted onto an organ of said mouse which corresponds to the human organ from which said tissue is originally obtained; and

having sufficient immuno-deficiency to allow said transplanted neoplastic tissue to grow and mimic the progression of the neoplastic disease in the human donor.

2. A nude mouse model according to claim 1 wherein said neoplastic tissue is selected from breast tissue, ovarian tissue or pleural tissue.

3. A nude mouse model according to claim 2 wherein said neoplastic tissue is obtained from human breast tissue.

4. A nude mouse model according to claim 3 wherein said human neoplastic breast tissue is implanted in the mammary fat pad of the mouse.

5. A nude mouse model according to claim 2 wherein said neoplastic tissue is obtained from human ovarian tissue.

6. A nude mouse model according to claim 5 wherein said human neoplastic ovarian tissue is implanted in the ovarian capsule of the mouse.

7. A nude mouse model according to claim 5 wherein said human neoplastic ovarian tissue is transplanted by securing to the surface of the mouse ovary at least two pieces of neoplastic tissue in close proximity to each other.

8. A nude mouse model according to claim 2 wherein said neoplastic tissue is obtained from human pleural tissue.

9. A nude mouse model according to claim 8 wherein said neoplastic tissue is implanted in the parietal pleura of the mouse.

10. A nude mouse model according to claim 9 wherein said neoplastic tissue is implanted in the visceral pleura of the mouse.

11. A method of generating a nude mouse model for human neoplastic disease, said method comprising:

transplanting histologically intact human neoplastic tissue of at least 1 mm³ in size onto an organ of a nude mouse which corresponds to the human organ from which said tissue is originally obtained; and

allowing said transplanted tissue to grow and mimic progression of the neoplastic disease in the human donor.

12. A method of generating a nude mouse according to claim 11 wherein said human neoplastic tissue is selected from breast tissue, ovarian tissue or pleural tissue.