Biosensor and method for producing the same

• US 5,575,895 A
• Filed: 05/30/1995
• Issued: 11/19/1996
• Est. Priority Date: 06/02/1994
• Status: Expired due to Term

• Current Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Ikeda, Shin, Yoshioka, Toshihiko, Nankai, Shiro, Miyahara, Mariko
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US08/452,774
• Time in Patent Office: 539 Days
• Field of Search: 204/403, 204/418, 204/412, 435/288, 435/291, 435/817
• US Class Current: 204/403.1
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A biosensor for quantification of a specific component contained in various biological samples comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode which are provided on the electrically insulating base plate, a reaction layer formed on the electrode system including at least an oxido-reductase, and an enclosure member having a hollow space constituting a sample supplying channel on the electrically insulating base plate, wherein substantially the whole of the reaction layer is exposed to the hollow space.

Claims

1. A biosensor comprising:

an electrically insulating base plate,

an electrode system including a working electrode and a counter electrode which are provided on a principal face of said electrically insulating base plate,

a reaction layer including at least an oxido-reductase, and

an enclosure member having a hollow space constituting a sample supplying channel having a width which is less than the width of said electrode system on said electrically insulating base plate,

wherein substantially the entire electrode system is located on the bottom of said hollow space and substantially the entire reaction layer is exposed to said hollow space.

2. The biosensor in accordance with claim 1, wherein said reaction layer comprises an electron acceptor.

3. The biosensor in accordance with claim 1, wherein said reaction layer further comprises a hydrophilic polymer.

4. The biosensor in accordance with claim 1, wherein said enclosure member comprises a spacer having a slot with an open end which serves as a sample supplying inlet on its tip end and a cover plate laminated with said spacer.

5. The biosensor in accordance with claim 4, wherein said spacer has an arcuate part which is provided over said electrode system and is shaped in conformity with an outer shape of said electrode system.

6. The biosensor in accordance with claim 1, wherein a part of the bottom of said hollow space which is on said electrode system is substantially in conformity with an outer shape of said electrode system.

7. The biosensor in accordance with claim 1, wherein said reaction layer comprises a carrier for carrying at least said oxido-reductase.

8. The biosensor in accordance with claim 1, wherein said reaction layer is formed on said electrode system in contact with said electrode system.

9. A method for producing a biosensor comprising the steps of:

forming an electrode system including a working electrode and a counter electrode on an electrically insulating base plate,

partitioning said electrically insulating base plate to define a section wherein substantially the entire electrode system is to be exposed by combining an enclosure member with said electrically insulating base plate, and

forming a reaction layer including at least an oxido-reductase only in said section defined in the previous step.

10. The method for producing a biosensor in accordance with claim 9, wherein said step of forming said reaction layer comprises forming a reaction layer including a hydrophilic polymer and an oxido-reductase.

11. The method for producing a biosensor in accordance with claim 9, wherein said step of forming said reaction layer comprises forming a reaction layer including an oxido-reductase and an electron acceptor.

12. The method for producing a biosensor in accordance with claim 9, wherein said step of forming said reaction layer comprises forming a reaction layer including a hydrophilic polymer, an oxido-reductase and an electron acceptor.

13. The method for producing a biosensor in accordance with claim 9 wherein the enclosure member includes a spacer, the partitioning step further including combining the spacer with the base plate, wherein the reaction layer forming step occurs after the combining step.

14. A method for producing a biosensor comprising the steps of:

forming an electrode system including a working electrode and a counter electrode on an electrically insulating base plate,

partitioning said electrically insulating base plate to define a section wherein substantially the entire electrode system is to be exposed by bringing a spacer into contact with said electrically insulating base plate,

forming a reaction layer including at least an oxido-reductase only in said section defined in the previous step, and

bringing a cover into contact with said spacer.

15. The method for producing a biosensor in accordance with claim 14, wherein said spacer has a slot with an open end which serves as a sample supplying inlet on its tip end.

16. The method for producing a biosensor in accordance with claim 15, wherein said spacer has an arcuate part which is provided over said electrode system and is shaped in conformity with an outer shape of said electrode system.

17. The method for producing a biosensor in accordance with claim 14 wherein the partitioning step further includes combining the spacer with the base plate, wherein the reaction layer forming step occurs after the combining step.

18. A method for producing a biosensor comprising the steps of:

forming an electrode system including a working electrode and a counter electrode on an electrically insulating base plate,

partitioning said electrically insulating base plate to define a section wherein substantially the entire electrode system is to be exposed by bringing a spacer into contact with said electrically insulating base plate,

forming a reaction layer comprising a carrier carrying at least an oxido-reductase only in said section defined in the previous step, and

bringing a cover into contact with said spacer.

19. The method for producing a biosensor in accordance with claim 18, wherein said carrier carries an electron acceptor.

20. The method for producing a biosensor in accordance with claim 18 wherein the partitioning step further includes combining the spacer with the base plate, wherein the reaction layer forming step occurs after the combining step.