Feed and orientation mechanism in automated analyzer

• US 5,582,796 A
• Filed: 06/01/1995
• Issued: 12/10/1996
• Est. Priority Date: 03/04/1991
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: CIBA Vision Corporation (Bayer AG)
• Inventors: Whitesel, Mary B., Lewis, Scott C., Carey, Glen A.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Le, Long V.
• Application Number:
  US08/456,992
• Time in Patent Office: 558 Days
• Field of Search: 422/63, 422/65, 422/67, 422/99, 422/104, 436/43, 436/47, 436/807, 198/393, 198/550.01, 198/443, 441/276, 441/304
• US Class Current: 422/65
• CPC Class Codes: B01L 2300/0854 Double walls
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 2035/00386 using fluid heat transfer medium
• CPC Class Codes: G01N 2035/00396 where the fluid is a liquid
• CPC Class Codes: G01N 2035/00524 Mixing by agitating sample carrier
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/0441 for samples
• CPC Class Codes: G01N 2035/0443 for reagents
• CPC Class Codes: G01N 2035/0465 Loading or unloading the conveyor
• CPC Class Codes: G01N 2035/0484 Belt or chain
• CPC Class Codes: G01N 2035/0486 Gearing, cams
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/021 having a flexible chain, e.g. "cartridge belt", conveyor for reaction cells or cuvettes
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y02A 90/10 Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
• CPC Class Codes: Y10S 215/01 Fins
• CPC Class Codes: Y10S 215/03 : Medical
• CPC Class Codes: Y10S 215/08 : Mixing
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis
• CPC Class Codes: Y10T 436/113332 : with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114165 : with step of insertion or removal from test line
• CPC Class Codes: Y10T 436/114998 : with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/119163 : with aspirator of claimed structure
• CPC Class Codes: Y10T 436/25 : including sample preparation
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

An analyzer for performing automated assay testing. The analyzer includes a storage and conveyor system for conveying cuvettes to an incubation or processing conveyor, a storage and selection system for test sample containers, a storage and selection system for reagent containers, sample and reagent aspirating and dispensing probes, a separation system for separating bound from unbound tracer or labeled reagent, a detection system and date collection/processing system. All of the subunits of the machine are controlled by a central processing unit to coordinate the activity of all of the subunits of the analyzer. The analyzer is specifically suited for performing heterogeneous binding assay protocols, particularly immunoassays.

Claims

1. A feed and orientation mechanism for a cuvette in an analyzer system to assay a test sample, said cuvette is generally rectangular in cross-section and has four side walls, a bottom wall, a top opening and a pair of oppositely extending flanges which extend laterally from two opposite walls of said side walls adjacent said top opening, said feed and orientation mechanism comprising:

(a) a hopper for containing a plurality of cuvettes in a random orientation with respect to each other and to the hopper, said hopper having a top opening, a bottom wall, side, walls and an opening in the bottom wall,

(b) a feeder conveyor which has a section which travels upwardly through the opening in the bottom wall to a first point at the top opening,

(c) a plurality of spaced projections on one flat side of said conveyor, each of said projections being adapted for supporting a cuvette on one of its side walls to a first point at the top opening of said hopper,

(d) an orientation chute which extends downwardly at an angle from said first point to a second point, said orientation chute having a pair of upper parallel slide surfaces which are separated by a slot which has a longitudinal sliding axis for receiving said cuvette from said conveyor belt at said first point so that said flanges of said cuvette are parallel with the longitudinal axis of the slot and are supported on said slide surfaces and said cuvette extends downwardly in said slot to enable said cuvette to slide downwardly on said chute along said axis from said first point to said second point,

(e) an orientation tube which extends vertically downwardly from said second point to a third point, said tube having a bottom opening, a top opening for receiving a cuvette from said orientation chute so that said cuvette falls through said tube to said third point,

(f) an event conveyor for conveying said cuvettes from said third point for subsequent use of said cuvettes by said analyzer, and

(g) control means for coordinating the feeder conveyor and the event conveyor.

2. In an analyzer system as recited in claim 1, wherein said orientation chute extends at a substantial angle relative to a plane of the event conveyor from one side of said event conveyor, said cuvette is conveyed so that said flanges are parallel with a path of travel of said event cuvette along said conveyor so that a path of travel of said cuvette along said sliding axis is at a substantial angle relative to the plane of the path of travel of said cuvette along said event conveyor and, wherein said tube has a twisted inner wall surface for causing each cuvette to rotate about its vertical axis as it falls through the tube, so that when the cuvette reaches said third point, the flanges of the cuvette are parallel with the path of travel of said cuvette along said event conveyor.

3. A feed and orientation mechanism as recited in claim 1, further comprising:

(a) a mixing block which extends upwardly through the bottom opening of said hopper adjacent said projections, said mixing block having an upper guide surface, and

(b) a drive actuator for vertically reciprocating said block for agitating said cuvettes and for orienting and guiding said cuvettes onto said projections by said guide surface.

4. A feed and orientation mechanism as recited in claim 3, wherein said conveyor and said drive actuator are driven in synchronism by control means so that when said mixing block moves upwardly at the same speed as said feeder conveyor during an upward portion of the reciprocating movement of said block.

5. A feed and orientation mechanism as recited in claim 3, wherein said feeder conveyor has a back side and a front side and, wherein said orientation chute is in front of said conveyor and said projections extend at a downward angle from said back side to said front side on the section of said feeder conveyor which extends upwardly from the opening in said bottom wall to said top opening so that when a cuvette reaches said first point it slides downwardly along the projection on which it rests to said orientation chute.

6. A feed and orientation mechanism as recited in claim 5, wherein the upper guide surface of said mixing block is at the same angle as said projections.

7. A feed and orientation mechanism as recited in claim 3, wherein a section of said feeder conveyor which extends upwardly from the bottom wall of the hopper is at an angle to the vertical so that said projections face upwardly and said mixing block is parallel with said conveyor section as it reciprocates.

8. A feed and orientation mechanism as recited in claim 1, wherein each of said slide surfaces extends at a downward angle toward said slot within a plane which is transverse to an axis which extends between said first and second points.

9. A feed and orientation mechanism as recited in claim 1, wherein said orientation chute further comprises a stop at said second point for preventing a cuvette from sliding beyond a lower end of the chute at said second point, and wherein said slot is sufficiently widened adjacent said stop to form an access opening for said cuvette above the top opening of said orientation tube to enable said cuvette to drop through said access opening into the top opening of said orientation tube.

10. A feed and orientation mechanism as recited in claim 9, wherein a pair of opposed vertical guide surfaces extend between said access opening and the top opening of said orientation tube, one of said guide surfaces having an upwardly facing jutting surface which slopes downwardly toward the other of said guide surfaces and the other of said guide surfaces having a recessed portion which forms a downwardly facing undercut surface which is opposite said jutting surface so that one flange of said cuvette strikes said jutting surface after it falls through said access opening to deflect said cuvette toward said recessed portion so that the other flange of said cuvette is positioned below said undercut surface to prevent said cuvette from flipping to an inverted orientation as it falls, thereby insuring that said cuvette enters the top opening of said orientation tube in an upright orientation.

11. A feed and orientation mechanism as recited in claim 1, wherein cuvettes are delivered by said mechanism to said third point at a greater rate than they are conveyed from said third point by said event conveyor, and said feeder conveyor is operated by drive means comprising:

(a) an electric motor which is drivingly connected to said feeder conveyor, and

(b) electrical control means for selectively actuating said electric motor, said control means comprising a sensor in said orientation tube for sensing when cuvettes have accumulated in said orientation tube to a predetermined level and for stopping said electric motor when said orientation tube has been filled with cuvettes to said predetermined level, and cuvette counting means for starting said electric motor when a predetermined number of cuvettes have been conveyed from said third point after the electric motor has been stopped.

12. A feed and orientation mechanism as recited in claim 11, wherein said sensor is an optical reflectance sensor.

13. A feed and orientation mechanism for a cuvette which is generally rectangular in cross-section and which has four side walls, a bottom wall, a top opening, and a pair of oppositely extending flanges which extend laterally from two opposite walls of said side walls adjacent said top opening, said feed and orientation mechanism comprising:

(a) a hopper for containing a plurality of cuvettes in a random orientation with respect to each other and to the hopper,

(b) a feeder convey or for conveying cuvettes in a substantially prone orientation from said hopper to a first point at a top of said hopper,

(c) an orientation chute which extends at a downward angle from said first point to a second point for guiding cuvettes in a sliding free fall along a sliding axis from said first point to said second point, said cuvettes being suspended by their flanges along said chute so that said cuvettes are substantially upright as they travel from said first point to said second point,

(d) an orientation tube for guiding said cuvettes in an upright orientation in a free fall from said second point to a third point,

(e) an event conveyor for conveying said cuvettes from said third point for subsequent use of said cuvettes, and

(f) control means for coordinating the feeder conveyor and the event conveyor.

14. A feeder and orientation mechanism as recited in claim 13, wherein said orientation chum extends at a substantial angle relative to a plane of said event conveyor from one side of said event conveyor, said cuvette is conveyed so that said flanges are parallel with a path of travel of said cuvette along said event conveyor so that a path of travel of said cuvette along said sliding axis is at a substantial angle relative to the plane of the path of travel of said cuvette along said event conveyor and, wherein said tube has a twisted inner wall surface for causing each cuvette to rotate about its vertical axis as it falls through the tube, so that when the cuvette reaches said third point, the flanges of the cuvette are parallel with the path of travel of said cuvette along said event conveyor.