Oxygen electrode, biosensor and processes for manufacturing same

• US 5,589,133 A
• Filed: 10/05/1994
• Issued: 12/31/1996
• Est. Priority Date: 07/20/1992
• Status: Expired due to Term

• Current Assignee: Fujitsu Limited
• Original Assignee: Fujitsu Limited
• Inventors: Suzuki, Hiroaki
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US08/318,209
• Time in Patent Office: 818 Days
• Field of Search: 422/79, 422/82.01, 422/82.02, 422/82.03, 422/82.04, 422/90, 436/138, 436/151, 204/403, 204/413, 204/414, 204/415, 204/426, 204/433, 435/7.91, 435/7.92, 435/29, 435/817, 435/287.5, 435/287.7
• US Class Current: 422/79
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An oxygen electrode comprising a plastic sheet, two or three electrode patterns formed on the plastic sheet, a paraffin layer formed on the peripheral portion of the plastic sheet for delineating a sensitive area, a carrier sheet impregnated with an electrolyte and placed on the central portion or the sensitive area of the plastic sheet, and a gas permeable membrane covering the carrier sheet and the paraffin layer. A biosensor may be also provided by further providing a microorganism or enzyme-immobilized layer on the gas permeable membrane of the above oxygen electrode.

Claims

1. An oxygen electrode comprising:

a plastic sheet having a planar surface;

an Ag/AgCl anode pattern on said surface of the plastic sheet;

a first electrode lead and pad pattern formed on the surface of the plastic sheet and electrically connected to said anode pattern;

a cathode pattern formed on said surface of the plastic sheet;

a second electrode lead and pad pattern formed on the surface of the plastic sheet and electrically connected to said cathode pattern;

a paraffin layer located on said surface of the plastic sheet over said patterns, said paraffin layer comprising a paraffin having a softening point of about 100°

to 150°

C. and being configured so as to delineate a sensitive area of the plastic sheet where portions of said anode and cathode patterns are exposed;

a carrier sheet formed of a natural or synthetic paper or a woven or non-woven cloth overlying said sensitive area and contacting said portions of the anode and cathode patterns; and

a gas permeable membrane overlying a first portion of the carrier sheet and the paraffin layer, said membrane being positioned and configured so that there is a second portion of the carrier sheet which is not covered by the membrane, whereby an electrolyte solution may be impregnated through said second portion of the carrier sheet.

2. An oxygen electrode according to claim 1, wherein the plastic sheet is formed from polyester, polycarbonate, polyimide or polystyrene.

3. An oxygen electrode according to claim 1, wherein the gas permeable membrane is formed from a fluorinated ethylene propylene, a silicone rubber, polypropylene or polytetrafluoroethylene.

4. An oxygen electrode comprising:

a plastic sheet having a planar surface;

an Ag/AgCl anode pattern on said surface of the plastic sheet;

a first electrode lead and pad pattern formed on the surface of the plastic sheet and electrically connected to said anode pattern;

a cathode pattern formed on said surface of the plastic sheet;

a second electrode lead and pad pattern formed on the surface of the plastic sheet and electrically connected to said cathode pattern;

a paraffin layer located on said surface of the plastic sheet over said patterns, said paraffin layer comprising a paraffin having a softening point of about 100°

to 150°

C. and being configured so as to delineate a sensitive area of the plastic sheet where portions of said anode and cathode patterns are exposed;

a carrier sheet formed of a natural or synthetic paper or a woven or non-woven cloth overlying said sensitive area and contacting said portions of the anode and cathode patterns, said carrier sheet being impregnated with a gel; and

a gas permeable membrane overlying a first portion of the carrier sheet and the paraffin layer, said membrane being positioned and configured so that there is a second portion of the carrier sheet which is not covered by said membrane, whereby an electrolyte solution may be impregnated through said second portion of the carrier sheet.

5. An oxygen electrode as set forth in claim 4, wherein said gel comprises agarose, gelatin, polyacrylamide or calcium alginate.

6. An biosensor comprising:

a plastic sheet having a planar surface;

an Ag/AqCl anode pattern on said surface of the plastic sheet;

a first electrode lead and pad pattern formed on the surface of the plastic sheet and electrically connected to said anode pattern;

a cathode pattern formed on said surface of the plastic sheet;

a second electrode lead and pad pattern formed on the surface of the plastic sheet and electrically connected to said cathode pattern;

a paraffin layer located on said surface of the plastic sheet over said patterns, said paraffin layer comprising a paraffin having a softening point of about 100°

to 150°

C. and being configured so as to delineate a sensitive area of the plastic sheet where portions of said anode and cathode patterns are exposed;

a carrier sheet formed of a natural or synthetic paper or a woven or non-woven cloth overlying said sensitive area and contacting said portions of the anode and cathode patterns;

a gas permeable membrane overlying a first portion of the carrier sheet and the paraffin layer, said membrane being positioned and configured so that there is a second portion of the carrier sheet which is not covered by said membrane, whereby an electrolyte solution can be impregnated through said second portion of the carrier sheet; and

an enzyme or microorganism-immobilized layer on the gas permeable membrane.

7. A process for manufacturing an oxygen electrode comprising:

preparing a plastic sheet having a planar surface;

forming an Ag/AqCl anode pattern on said surface of the plastic sheet;

forming a first electrode lead and pad pattern that is electrically connected to said anode pattern on the surface of the plastic sheet;

forming a cathode pattern on said surface of the plastic sheet;

forming a second electrode lead and pad pattern that is electrically connected to said cathode pattern on the surface of the plastic sheet and;

fusing a paraffin layer onto said surface of the plastic sheet over said patterns, said paraffin layer comprising a paraffin having a softening point of about 100°

to 150°

C.;

configuring said paraffin layer so as to delineate a sensitive area of the plastic sheet where portions of the anode and cathode patterns are exposed;

placing a carrier sheet formed of a natural or synthetic paper or a woven or non-woven cloth on the plastic sheet so as to overlie said sensitive area and contact said portions of the anode and cathode patterns;

placing a gas permeable membrane over a first portion of the carrier sheet and the paraffin layer, said membrane being positioned and configured so that there is a second portion of the carrier sheet which is not covered by said membrane; and

impregnating the carrier sheet with an electrolyte through said second portion of the carrier sheet.

8. A process according to claim 7, including forming the anode pattern by selective vacuum evaporation of a metal with a metal mask.

9. A process according to claim 7, including forming the cathode pattern by selective vacuum evaporation of a metal with a metal mask.

10. A process according to claim 7, including forming the anode and cathode patterns by selective vacuum evaporation of a metal with a metal mask.

11. A process for manufacturing an oxygen electrode comprising:

preparing a plastic sheet having a planar surface;

forming an Ag/AgCl anode pattern on said surface of the plastic sheet;

forming a first electrode lead and pad pattern that is electrically connected to said anode pattern on the surface of the plastic sheet;

forming a cathode pattern on said surface of the plastic sheet;

forming a second electrode lead and pad pattern that is electrically connected to said cathode pattern on the surface of the plastic sheet and;

fusing a paraffin layer onto said surface of the plastic sheet over said patterns, said paraffin layer comprising a paraffin having a softening point of about 100°

to 150°

C.;

configuring said paraffin layer so as to delineate a sensitive area of the plastic sheet where portions of the anode and cathode patterns are exposed;

placing a carrier sheet formed of a natural or synthetic paper or a woven or non-woven cloth on the plastic sheet so as to overlie said sensitive area and contact said portions of the anode and cathode patterns;

impregnating the carrier sheet with a gel;

placing a gas permeable membrane over a first portion of the carrier sheet and the paraffin layer, said membrane being positioned and configured so that there is a second portion of the carrier sheet which is not covered by said membrane; and

impregnating the carrier sheet with an electrolyte through said second portion of the carrier sheet.

12. A process as set forth in claim 11, wherein said gel comprises agarose, gelatin, polyacrylamide or calcium alginate.

13. A process for manufacturing a biosensor comprising:

preparing a plastic sheet having a planar surface;

forming an Ag/AgCl anode pattern on said surface of the plastic sheet;

forming a first electrode lead and pad pattern that is electrically connected to said anode pattern on the surface of the plastic sheet;

forming a cathode pattern on said surface of the plastic sheet;

forming a second electrode lead and pad pattern that is electrically connected to said cathode pattern on the surface of the plastic sheet and;

fusing a paraffin layer onto said surface of the plastic sheet over said patterns, said paraffin layer comprising a paraffin having a softening point of about 100°

to 150°

C.;

configuring said paraffin layer so as to delineate a sensitive area of the plastic sheet where portions of the anode and cathode patterns are exposed;

placing a carrier sheet formed of a natural or synthetic paper or a woven or non-woven cloth on the plastic sheet so as to overlie said sensitive area and contact said portions of the anode and cathode patterns;

placing a gas permeable membrane over a first portion of the carrier sheet and the paraffin layer, said membrane being positioned and configured so that there is a second portion of the carrier sheet which is not covered by said membrane;

forming an enzyme or microorganism-immobilized layer on the gas permeable membrane; and

impregnating the carrier sheet with an electrolyte through said second portion of the carrier sheet.

14. A biosensor comprising:

a plastic sheet having a planar surface;

an Ag/AgCl anode pattern on said surface of the plastic sheet;

a first electrode lead and pad pattern formed on the surface of the plastic sheet and electrically connected to said anode pattern;

a cathode pattern formed on said surface of the plastic sheet;

a second electrode lead and pad pattern formed on the surface of the plastic sheet and electrically connected to said cathode pattern;

a paraffin layer located on said surface of the plastic sheet over said patterns, said paraffin layer comprising a paraffin having a softening point of about 100°

to 150°

C. and being configured so as to delineate a sensitive area of the plastic sheet where portions of said anode and cathode patterns are exposed;

a carrier sheet formed of a natural or synthetic paper or a woven or non-woven cloth overlying said sensitive area and contacting said portions of the anode and cathode patterns, said carrier sheet being impregnated with a gel;

a gas permeable membrane overlying a first portion of the carrier sheet and the paraffin layer, said membrane being positioned and configured so that there is a second portion of the carrier sheet which is not covered by said membrane, whereby an electrolyte solution can be impregnated through said second portion of the carrier sheet; and

an enzyme or microorganism-immobilized layer on the gas permeable membrane.

15. An oxygen electrode as set forth in claim 14, wherein said gel comprises agarose, gelatin, polyacrylamide or calcium alginate.

16. A process for manufacturing a biosensor comprising:

preparing a plastic sheet having a planar surface;

forming an Ag/AgCl anode pattern on said surface of the plastic sheet;

forming a first electrode lead and pad pattern that is electrically connected to said anode pattern on the surface of the plastic sheet;

forming a cathode pattern on said surface of the plastic sheet;

forming a second electrode lead and pad pattern that is electrically connected to said cathode pattern on the surface of the plastic sheet and;

fusing a paraffin layer onto said surface of the plastic sheet over said patterns, said paraffin layer comprising a paraffin having a softening point of about 100°

to 150°

C.;

configuring said paraffin layer so as to delineate a sensitive area of the plastic sheet where portions of the anode and cathode patterns are exposed;

placing a carrier sheet formed of a natural or synthetic paper or a woven or non-woven cloth on the plastic sheet so as to overlie said sensitive area and contact said portions of the anode and cathode patterns;

impregnating the carrier sheet with a gel;

placing a gas permeable membrane over a first portion of the carrier sheet and the paraffin layer, said membrane being positioned and configured so that there is a second portion of the carrier sheet which is not covered by said membrane;

forming an enzyme or microorganism-immobilized layer on the gas permeable membrane; and

impregnating the carrier sheet with an electrolyte through said second portion of the carrier sheet.

17. A process as set forth in claim 16, wherein said gel comprises agarose, gelatin, polyacrylamide or calcium alginate.