Subcutaneous glucose electrode

• US 5,593,852 A
• Filed: 09/01/1994
• Issued: 01/14/1997
• Est. Priority Date: 12/02/1993
• Status: Expired due to Term

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated), Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Adam Heller, Michael V. Pishko
• Inventors: Pishko, Michael V., Heller, Adam
• Primary Examiner(s): Kight, John
• Assistant Examiner(s): Leary, Louise N.
• Application Number:
  US08/299,526
• Time in Patent Office: 866 Days
• Field of Search: 435/14, 435/4, 435/817, 435/25, 435/28, 435/289.1, 436/63, 436/74, 436/149
• US Class Current: 435/14
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10S 435/917 Aspergillus niger
• CPC Class Codes: Y10S 435/962 Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding

Abstract

A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow "one-point" in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.

Claims

1. A biosensor for measuring the concentration of a biochemical, comprising:

a non-corroding metal or carbon electrode;

a sensing layer comprising a redox polymer and a redox enzyme, adjacent to and in electrical contact with the electrode; and

a biocompatible layer overcoating the sensing layer, comprising a polymer and not less than 20% water by weight when in equilibrium with physiological body fluids.

2. The biosensor of claim 1, wherein the biocompatible layer is formed of cross-linked poly(ethylene oxide).

3. The biosensor of claim 1 wherein, said redox polymer is derived from poly(1-vinylimidazole) or a copolymer of (1-vinyl imidazole) bound to a metal ion selected from the group consisting of Os^3+/2+, Ru^3+/2+, and Fe^3+/2+.

4. The biosensor of claim 1 wherein, the redox potential of said redox polymer is not more reducing than about -0.15 V and not more oxidizing than about +0.15 V versus the standard calomel electrode in an aqueous solution at about pH7.4.

5. The biosensor of claim 1 further comprising a biochemical flux limiting layer.

6. The biosensor of claim 1, wherein said electrode is a wire, the wire substantially coated with an electrically insulating polymer and having a gap or recess in the coating exposing a bare, non-insulated surface of the wire, the insulating polymer containing less than 5% water by weight when in equilibrium with physiological body fluids at about 37°

C.

7. The biosensor of claim 6, wherein said exposed wire is recessed in the insulating polymer coat forming a channel in the insulating polymer coat.

8. The biosensor of claim 6, wherein said channel has a depth ranging from about 20 microns to about 1 mm.

9. A method for manufacturing the biosensor of claim 8, wherein said recessed channel is formed by electrolytic dissolution of part of the electrically conducting wire, with an oxidizing electrical potential of not less than 0.3 V versus the standard calomel electrode being maintained on the dissolving wire, said wire being immersed in an aqueous solution containing at least one anion selected from the group consisting of CN^-, Cl^-, Br^- and I^- at a concentration of at least approximately 0.1 M.

10. A method for measuring the concentration of a biochemical in an animal comprising:

contacting body fluid of the animal with the biosensor of claim 1 to generate an electrical signal; and

determining from the generated electrical signal the concentration of a biochemical in the body fluid.

11. The method of claim 10, wherein said body fluid is blood, plasma or serum.

12. The method of claim 10, wherein the measuring is intermittent.

13. The method of claim 10, wherein said contacting is implanting the electrode subcutaneously in said animal.

14. The method of claim 10, further comprising placing a reference electrode or combined reference and counter electrode on or in the skin of the animal.

15. The method of claim 10, wherein the biochemical to be detected is glucose.

16. The method of claim 10 wherein the biochemical to be detected is lactate.

17. A method of calibrating the biosensor of claim 1, comprising the steps of:

independently analyzing the concentration of a biochemical to be measured by the electrode of claim 1 in a single sample of fluid withdrawn from an animal, and

relating the electrical signal generated by the electrode to the concentration of biochemical in the single sample of withdrawn fluid.

18. A biosensor for detecting a biochemical comprising:

two or more non-corroding metal or carbon biochemical-responsive electrodes;

an electrically insulating polymer coating at least a part of each electrode;

a sensing layer adjacent to and in electrical contact with a part of each metal or carbon electrode that is not coated by the insulating polymer, the sensing layer comprising a redox enzyme and redox couple, neither of which is leached by a physiological buffer solution consisting of water, sodium chloride and sodium phosphates at a pH between 6.8 and 7.6 or by serum at about 37°

C., wherein the sensing layer generates an electrical signal in the presence of the biochemical; and

,

a biocompatible polymer coating on an outer surface of each of the electrodes.

19. The biosensor of claim 18, wherein said electrically insulating polymer is selected from the group consisting of polyimide, polyester, polyurethane and perfluorinated polymer.

20. The biosensor of claim 18, wherein the outer diameter of each electrode is smaller than 0.3 mm.

21. The biosensor of claim 18, wherein each of the biochemical-responsive electrodes further comprises an interference eliminating layer comprising a peroxidase enzyme.

22. A method for measuring the concentration of a biochemical in an animal comprising:

contacting body fluid of an animal with said two or more biochemical-responsive electrodes of claim 18 to generate electrical signals; and

determining from the generated electrical signals the concentration of a biochemical in the body fluid.

23. The method of claim 22, wherein said contacting is implanting the electrodes subcutaneously in said animal.

24. The method of claim 22 further comprising placing a reference electrode or combined reference and counter electrode on or in the skin of the animal.

25. The method of claim 22 wherein substantially simultaneous readings of said two or more electrodes are accepted as correctly measuring the concentration of the biochemical when the two or more readings do not differ by more than a specified percentage of the measured electrical signals.

26. The method of claim 22, wherein readings of said two or more electrodes that do not differ by more than about 20% or by more than about one standard deviation are accepted as correctly measuring the concentration of the biochemical.

27. The method of claim 22, wherein the biochemical is glucose or lactate.

28. A method of calibrating the biosensor of claim 18, comprising the steps of:

independently analyzing the concentration of a biochemical to be measured by the electrodes of claim 18 in a single sample of fluid withdrawn from an animal, and

relating the electrical signal generated by said two or more electrodes to the concentration of biochemical in the single sample of withdrawn fluid.

29. An electrode comprising:

a non-corroding, conducting wire coated with an insulating polymer;

a recess forming a channel in the insulating polymer coat, the recess bounded at one end by exposed wire and at an opposite end by the environment adjacent the coated wire;

a multilayered polymeric composition within said channel, the composition comprising;

a redox layer adjacent to and contacting said wire, the redox layer comprising a redox enzyme cross-linked to a redox polymer; and

a biocompatible polymer layer adjacent to the environment outside the coated wire.

30. The electrode of claim 29, further comprising an analyte diffusion limiting barrier layer positioned between said redox layer and said biocompatible layer.

31. A method for producing an in vivo biosensor to measure the concentration of an analyte, comprising the steps of:

coating a non-corroding metal or carbon wire with a biocompatible insulating polymer containing less than 5% water when in equilibrium with physiological body fluids at 37°

C. to form a coated wire;

etching said coated wire to form a recess, said recess devoid of metal or carbon;

immobilizing within said recess, adjacent to said wire, a redox composition comprising a redox enzyme and a redox polymer; and

overcoating the contents of the recess with a biocompatible polymer;

wherein said etched wire with said polymer overcoated recess and contents form a biosensor producing substantially no electrical signal output at zero concentration of the analyte to be measured, even in the presence of ascorbate, urate or acetaminophen in their normal concentration range in plasma, serum or blood.