Electrochemical sensors

• US 5,601,694 A
• Filed: 07/27/1995
• Issued: 02/11/1997
• Est. Priority Date: 06/27/1994
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: CIBA Vision Corporation (Bayer AG)
• Inventors: Edelman, Peter G., Flaherty, James E., Mason, Richard W., D'Orazio, Paul A., McCaffrey, Robert R., Dalzell, Bonnie C., Maley, Thomas C.
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US08/508,276
• Time in Patent Office: 565 Days
• Field of Search: 204/415, 204/403, 204/286, 204/290 R, 204/290 F, 204/294, 204/402, 128/635, 435/817, 435/288, 435/291
• US Class Current: 204/403.09
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10T 29/49117 Conductor or circuit manufacturing
• CPC Class Codes: Y10T 29/49204 Contact or terminal manufacturing
• CPC Class Codes: Y10T 29/53261 Means to align and advance work part
• CPC Class Codes: Y10T 29/53265 with work-holder for assembly

Abstract

A solid shale, multi-use electrochemical sensor having an electrically nonconductive substrate, a working electrode, and a semi-permeable membrane covering the working electrode. The working electrode includes an electrically conductive material adhered to a portion of the substrate. A first portion of the conductive material is covered with an electrically insulating dielectric coating, and a second portion of the conductive material is covered with an active layer. The active layer includes a catalytically active quantity of an enzyme carried by platinized carbon powder particles, which are distributed throughout the active layer. A sensor package for incorporating a sensor is provided.

Claims

1. An interference electrode, comprising:

an electrically nonconductive substrate; and

an electrically conductive material adhered to a portion of said substrate, a first portion of said electrically conductive material being covered with an electrically insulating dielectric coating, and a second portion of said electrically conductive material being covered with an inactive layer comprising an inactive protein immobilized onto platinized carbon powder particles, wherein said particles are distributed substantially uniformly throughout said inactive layer; and

a semi-permeable membrane covering said inactive layer.

2. The interference electrode according to claim 1, wherein the inactive protein is albumin.

3. The inactive electrode according to claim 1, wherein the inactive protein is bovine serum albumin.

4. An interference electrode, comprising:

an electrically conductive material, a first portion of said electrically conductive material being covered with an electrically insulating dielectric coating, and a second portion of said electrically conductive material being covered with an inactive layer comprising an inactive protein immobilized onto platinized carbon powdered particles, wherein said particles are distributed substantially uniformly throughout said inactive layers; and

a semi-permeable membrane covering said inactive layer.

5. The interference electrode according to claim 4, wherein the inactive protein is albumin.

6. The interference electrode according to claim 5, further comprising a substrate for said electrically conductive material.

7. The interference electrode according to claim 4, wherein the inactive protein is bovine serum albumin.

8. The interference electrode according to claim 7, further comprising a substrate for said electrically conductive material.

9. The interference electrode according to claim 4, further comprising a substrate for said electrically conductive material.