Apparatus for measuring analytes in a fluid sample

• US 5,607,565 A
• Filed: 03/27/1995
• Issued: 03/04/1997
• Est. Priority Date: 03/27/1995
• Status: Expired due to Fees

• Current Assignee: Coulter International Corp., Warren W. Kurz
• Original Assignee: Coulter Corporation (Danaher Corporation)
• Inventors: Azarnia, Roobik, Russell, Thomas R., Fuentes, Susan M.
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US08/410,997
• Time in Patent Office: 708 Days
• Field of Search: 204/403, 204/409, 204/153.12, 204/415, 204/153.17, 204/401, 204/412, 422/68.1, 422/82.01, 422/82.03, 435/4, 435/14, 435/291, 435/288, 435/817, 436/52
• US Class Current: 204/403.09
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream

Abstract

A method and apparatus provide a system for measuring a measurable component in a sample. A sample is introduced into a chamber. A permeable membrane to the measurable component allows the component to pass into a second chamber. In the second chamber the component is measured directly or reacted in the presence of an enzyme to generate a detectable product detected by a detector therein. The second chamber is then flushed. The second chamber is only of a size sufficient to contain enough liquid to wet the detector therein.

Claims

1. An apparatus for measuring the amount of a measurable substance in a sample, comprising:

first chamber means having an inlet and an outlet for having a sample introduced thereinto and removed therefrom, said first chamber means having three chambers interconnected by a flow path, two of said three chambers each have an electrode disposed therein, each chamber being of a size sufficient only for wetting their respective electrodes by an amount sufficient to conduct electrochemical detection of substances;

membrane means partially defining second chamber means located relative to said first chamber means for allowing a first predetermined substance in said sample to pass therethrough;

detecting means disposed in said second chamber for detecting one of said first predetermined substance and a product thereof which comprises a second predetermined substance, in said second chamber; and

said second chamber means having an inlet and an outlet for supplying and removing from said second chamber a third substance capable of carrying in suspension at least one of said first predetermined substance and said second predetermined substance, while being retained in said second chamber by said membrane, and for flushing said second chamber means, whereby one of said first and second substances is the measurable substance and said inlet and outlet means allowing flushing of said second chamber after measurement of said measurable substance has been conducted.

2. The apparatus as in claim 1 wherein said inlet means and outlet means of said second chamber means are for supplying and removing an enzyme from said second chamber means.

3. The apparatus as in claim 1 wherein said detecting means is an electrode and said second chamber means is only of a size sufficient to wet said electrode to allow electrochemical detecting to occur.

4. The apparatus as in claim 1 further comprising means for detecting substances which interfere with detection of predetermined substances to be detected.

5. The apparatus as in claim 4, further comprising a third chamber having said means for detecting substances which interface with detection of predetermined substances to be detected, a second membrane defining at least one wall of said third chamber for allowing said substances which interfere to pass therethrough into said third chamber, and buffer inlet means and buffer outlet means for flushing said third chamber of said substances which interfere after each measurement.

6. The apparatus as defined by claim 5 wherein said measurement chamber has a volume less than about 100 microliters.

7. The apparatus as in claim 1 further comprising means for receiving a signal from said detecting means and for processing said signal and generating an output indicative of the amount of predetermined substance in a sample being analyzed.

8. The apparatus as in claim 1 wherein said second chamber means has an electrode disposed therein and is separated from said chamber of said first chamber means having no electrode by a permeable membrane, and wherein said second chamber means is of a size only sufficient to wet said electrode therein an amount sufficient to conduct electrochemical detection of substances.

9. The apparatus as in claim 1 further comprising heating means for controlling the rate of any reactions conducted therein.

10. The apparatus as in claim 1 further comprising buffer inlet means for injecting buffer solution into said first chamber means, and buffer outlet means for removing buffer solution from said first chamber means.

11. An apparatus for measuring the amount of an analyte in a fluid sample, said apparatus comprising (a) a housing defining a sample-receiving chamber and a measurement chamber separated by a semipermeable membrane, said sample-receiving chamber having an inlet for admitting said fluid sample into said sample-receiving chamber, said membrane transmitting said analyte from said sample-receiving chamber to said measurement chamber, said measurement chamber containing an enzyme which reacts with said analyte to produce a measurable byproduct, and (b) a detector for detecting said measurable byproduct, said apparatus being characterized in that:

a) said measurement chamber has a volume of less than 1.00 microliters and has an inlet for selectively admitting said enzyme into said second chamber;

b) said detector is positioned within said measurement chamber;

, and

c) means are provided for flushing said measurement chamber of said analyte, enzyme and/or byproduct.

12. The apparatus as defined by claim 11 further characterized in that a second detector is arranged in said sample-receiving chamber, and circuit means are provided for compensating the output of the detector positioned within said measurement chamber with the output of said second detector.

13. The apparatus as defined by claim 11 further characterized in that said second detector is surrounded by a second semipermeable membrane.

14. The apparatus as defined by claim 11 further characterized in that heating means are provided within said housing for controlling the rate of any chemical reaction within said measurement chamber.