Etiological agent for porcine enteritis

• US 5,610,059 A
• Filed: 11/09/1992
• Issued: 03/11/1997
• Est. Priority Date: 11/09/1992
• Status: Expired due to Term

• Current Assignee: Arizona Board of Regents (University of Arizona)
• Original Assignee: University of Arizona
• Inventors: Joens, Lynn A., Glock, Robert D.
• Primary Examiner(s): Naff, David M.
• Assistant Examiner(s): Larson, Kristin K.
• Application Number:
  US07/973,018
• Time in Patent Office: 1,583 Days
• Field of Search: 435/240.2, 435/252.1, 435/822, 424/93.3, 424/93.4, 424/93.7
• US Class Current: 435/252.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/195 from bacteria
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: Y10S 435/822 using bacteria or actinomycetales

Abstract

A novel intracellular organism isolated from enterocytes of pigs affected by Porcine Proliferative Enteritis (PPE) may be cultured by passage in a permissive host cell and may be purified from lysates of such cells. On inoculation, the organism of the present invention causes PPE-like symptoms in pigs. The infectious PPE-causing agent may be used to develop a whole organism bacterin or subunit preparation effective to prevent PPE. In addition, the cultured isolate may be used to prepare a battery of therapeutic and diagnostic monoclonal antibodies directed against the PPE-causing agent. Further, by isolating the genetic material from the agent, various useful fusion proteins may be prepared and the genetic makeup of the organism may be determined.

Claims

1. A biologically pure culture of a mammalian host cell infected by an obligate intracellular bacterium that causes porcine proliferative enteritis after inoculation into pigs having all of the identifying characteristics of ATCC Accession No. 55370.

2. A biologically pure culture as claimed in claim 1 wherein the mammalian host cell is the human intestinal cell line Henle 407.

3. A biologically pure culture as claimed in claim 1 wherein the obligate intracellular bacterium that causes porcine proliferative enteritis is a bacterium having dimensions in the range of 0.24-0.27 micron×

1.4-2.4 micron.

4. A biologically pure culture as claimed in claim 1 wherein the obligate intracellular bacterium that causes porcine proliferative enteritis is isolated from enterocytes of a pig having porcine proliferative enteritis.