Detection of both an antibody and an antigen in a single sample aliquot

  • US 5,627,026 A
  • Filed: 12/27/1994
  • Issued: 05/06/1997
  • Est. Priority Date: 07/14/1988
  • Status: Expired due to Term
First Claim
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1. A method for detecting an antibody and an antigen in a single biological sample, said method comprising:

  • (a) providing an antigen which selectively forms a first immune complex with a sample antibody, said antigen being directly bound to a solid support at a first location;

    (b) providing an antibody which selectively forms a second immune complex with a sample antigen, said antibody being directly bound to said solid support at a second location;

    (c) contacting said first location on said solid support with at least a portion of a biological sample under conditions whereby said first immune complex can form and contacting said second location on said solid support with at least a portion of said sample under conditions whereby said second immune complex can form;

    (d) washing unbound material from said first location and from said second location; and

    (e) separately detecting whether said first immune complex is formed and whether said second immune complex is formed, said first immune complex being detected with a labelled antigen which selectively binds to said first complex and said second immune complex being detected by adding a labelled antibody which selectively binds to said second complex, said labelled antibody being presented to both said first and said second locations.

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