Detection of both an antibody and an antigen in a single sample aliquot
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1. A method for detecting an antibody and an antigen in a single biological sample, said method comprising:
- (a) providing an antigen which selectively forms a first immune complex with a sample antibody, said antigen being directly bound to a solid support at a first location;
(b) providing an antibody which selectively forms a second immune complex with a sample antigen, said antibody being directly bound to said solid support at a second location;
(c) contacting said first location on said solid support with at least a portion of a biological sample under conditions whereby said first immune complex can form and contacting said second location on said solid support with at least a portion of said sample under conditions whereby said second immune complex can form;
(d) washing unbound material from said first location and from said second location; and
(e) separately detecting whether said first immune complex is formed and whether said second immune complex is formed, said first immune complex being detected with a labelled antigen which selectively binds to said first complex and said second immune complex being detected by adding a labelled antibody which selectively binds to said second complex, said labelled antibody being presented to both said first and said second locations.
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Abstract
A method for detecting the presence of antibody to FIV in a biological sample. The method includes the steps of providing a first antigen having a first epitope recognized by the antibody, the first antigen being detectable; contacting the first antigen with the sample under conditions under which the antibody can bind to the first antigen to form an immune complex; and detecting the immune complex. An ELISA test for FIV, using purified FIV antigen, is also described.
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15 Claims
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1. A method for detecting an antibody and an antigen in a single biological sample, said method comprising:
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(a) providing an antigen which selectively forms a first immune complex with a sample antibody, said antigen being directly bound to a solid support at a first location; (b) providing an antibody which selectively forms a second immune complex with a sample antigen, said antibody being directly bound to said solid support at a second location; (c) contacting said first location on said solid support with at least a portion of a biological sample under conditions whereby said first immune complex can form and contacting said second location on said solid support with at least a portion of said sample under conditions whereby said second immune complex can form; (d) washing unbound material from said first location and from said second location; and (e) separately detecting whether said first immune complex is formed and whether said second immune complex is formed, said first immune complex being detected with a labelled antigen which selectively binds to said first complex and said second immune complex being detected by adding a labelled antibody which selectively binds to said second complex, said labelled antibody being presented to both said first and said second locations. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for detecting an antibody to an antigen of a first feline pathogen and an antigen of a second feline pathogen in a single biological sample, said first and second feline pathogens being independently selected from the group consisting of Feline Leukemia Virus (FeLV) and Feline Immune Deficiency Virus (FIV), said method comprising:
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(a) providing an antigen which selectively forms a first immune complex with a sample antibody, said antigen being directly bound to a solid support at a first location; (b) providing an antibody which selectively forms a second immune complex with a sample antigen, said antibody being directly bound to said solid support at a second location; (c) contacting said first location on said solid support with at least a portion of a biological sample under conditions whereby said first immune complex can form and contacting said second location on said solid support with at least a portion of said sample under conditions whereby said second immune complex can form; (d) washing unbound material from said first location and from said second location; and (e) separately detecting whether said first immune complex is formed and whether said second immune complex is formed, said first immune complex being detected with a labelled antigen which selectively binds to said first complex and said second immune complex being detected by adding a labelled antibody which selectively binds to said second complex, said labelled antibody being presented to both said first and said second locations. - View Dependent Claims (8)
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9. A kit comprising:
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a) a solid support comprising an antigen which is capable of selectively forming a first immune complex with a sample antibody, said antigen being directly bound to a solid support at a first location; and
an antibody, said antibody being capable of selectively forming a second immune complex with a sample antigen, said antibody being directly bound to said solid support at a second location,wherein said locations are positioned so that antigen may be contacted with at least a portion of a biological sample whereby said first immune complex can form and said antibody may be contacted with at least a portion of said sample, whereby said second immune complex can form; b) a complex detector comprising a labelled antigen which selectively binds to said first immune complex, and c) a labelled antibody which selectively binds to said second immune complex when said labelled antibody is presented to both said first and said second locations. - View Dependent Claims (10, 11, 12, 13)
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14. A kit comprising:
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a) a solid support comprising an antigen of a first feline pathogen, wherein said antigen is capable of selectively forming a first immune complex with a sample antibody, said antigen being directly bound to a solid support at a first location; and
an antibody to an antigen of a second feline pathogen , wherein said antibody is capable of selectively forming a second immune complex with a sample antigen, said antibody being directly bound to said solid support at a second location,wherein said antigen may be contacted with at least a portion of a biological sample under conditions whereby said first immune complex can form and said antibody may be contacted with at least a portion of said sample, whereby said second immune complex can form; b) a complex detector comprising a labelled antigen which selectively binds to said first immune complex, and c) a labelled antibody which selectively binds to said second immune complex when said labelled antibody is presented to both said first and said second locations. - View Dependent Claims (15)
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Specification