Method of identifying a nonparaffinophilic microorganism using various milieus and an associated apparatus

• US 5,639,675 A
• Filed: 11/09/1995
• Issued: 06/17/1997
• Est. Priority Date: 11/09/1995
• Status: Expired due to Fees

• Current Assignee: Infectech, Inc.
• Original Assignee: Infectech, Inc.
• Inventors: Felder, Mitchell S., Ollar, Robert-A.
• Primary Examiner(s): Kight, John
• Assistant Examiner(s): LEARY, LOUISE N
• Application Number:
  US08/555,742
• Time in Patent Office: 586 Days
• Field of Search: 435/29, 435/34, 435/4, 435/30, 435/36, 435/42, 436/63, 436/74
• US Class Current: 435/29
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/975 Kit

Abstract

A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient. The method includes providing a receptacle containing an aqueous solution and adjusting the solution to mimic the in vivo clinical conditions of the patient. The method further includes inoculating the solution with the specimen and then placing in the receptacle a slide coated with a carbon source to bait the nonparaffinophilic microorganism. The slide is then analyzed after exposure to the specimen to determine the presence or absence of the nonparaffinophilic microorganism. An associated apparatus is also disclosed.

Claims

1. A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient, said method comprising:

providing a receptacle containing an aqueous solution;

adjusting said aqueous solution to mimic the in vivo clinical conditions of said patient;

inoculating said solution with said specimen;

placing into said receptacle a slide coated with a carbon source; and

analyzing said slide after exposure to said specimen to determine the presence or absence of said nonparaffinophilic microorganism.

2. The method of claim 1, including

employing as said specimen one selected from the group consisting of blood, stomach fluid, urine, cerebral spinal fluid, nasopharyngeal mucosa and saliva.

3. The method of claim 1, including

effecting said adjusting of said aqueous solution by altering the pH of said aqueous solution.

4. The method of claim 3, including

altering said pH of said aqueous solution by adding chemicals to said aqueous solution.

5. A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient, said method comprising:

providing a receptacle containing an aqueous solution;

adjusting said aqueous solution to mimic the in vivo clinical conditions of said patient;

inoculating said solution with said specimen;

placing into said receptacle a slide coated with a carbon source;

analyzing said slide after exposure to said specimen to determine the presence or absence of said nonparaffinophilic microorganism;

altering said pH of said aqueous solution by adding chemicals to said aqueous solution; and

employing as said chemicals one selected from the group consisting of HCl, KOH and NaOH.

6. A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient, said method comprising:

providing a receptacle containing an aqueous solution;

adjusting said aqueous solution to mimic the in vivo clinical conditions of said patient;

inoculating said solution with said specimen;

placing into said receptacle a slide coated with a carbon source;

analyzing said slide after exposure to said specimen to determine the presence or absence of said nonparaffinophilic microorganism; and

effecting said adjusting of said aqueous solution by adding chemicals to said solution to mimic the electrolyte level in said patient'"'"'s blood.

7. The method of claim 6, including

employing as said chemicals at least one of the group consisting of sodium, potassium, chloride, magnesium, phosphate and calcium.

8. A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient, said method comprising:

providing a receptacle containing an aqueous solution;

adjusting said aqueous solution to mimic the in vivo clinical conditions of said patient;

inoculating said solution with said specimen;

placing into said receptacle a slide coated with a carbon source;

analyzing said slide after exposure to said specimen to determine the presence or absence of said nonparaffinophilic microorganism; and

employing as said slide one coated with a gelatinous matrix containing said carbon source.

9. A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient, said method comprising:

providing a receptacle containing an aqueous solution;

adjusting said aqueous solution to mimic the in vivo clinical conditions of said patient;

inoculating said solution with said specimen;

placing into said receptacle a slide coated with a carbon source;

analyzing said slide after exposure to said specimen to determine the presence or absence of said nonparaffinophilic microorganism; and

providing said slide by first adhering to said slide a plurality of gel beads and then bonding to said gel beads said carbon source.

10. The method of claim 9, wherein

said carbon source is ionically bound to said gel beads.

11. The method of claim 9, wherein

said carbon source is affinity bound to said gel beads.

12. The method of claim 9, including

adhering said gel beads to said slide by means of an adhesive.

13. The method of claim 12, including

employing as said adhesive collodion.

14. A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient, said method comprising:

providing a receptacle containing an aqueous solution;

adjusting said aqueous solution to mimic the in vivo clinical conditions of said patient;

inoculating said solution with said specimen;

placing into said receptacle a slide coated with a carbon source;

analyzing said slide after exposure to said specimen to determine the presence or absence of said nonparaffinophilic microorganism; and

employing as said carbon source one or more of the group consisting of glucose, fructose, glycenol, mannitol, asparagine and casein.