Sterile flow cytometer and sorter with mechanical isolation between flow chamber and sterile enclosure

• US 5,641,457 A
• Filed: 04/25/1995
• Issued: 06/24/1997
• Est. Priority Date: 04/25/1995
• Status: Expired due to Term

• Current Assignee: Novartis Ag
• Original Assignee: Systemax Incorporated
• Inventors: Medeiros, David J., Lewis, Hugh, Vardanega, Michael H., Tichenor, Edie, Joubran, John, Swan, Raymond
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US08/428,693
• Time in Patent Office: 791 Days
• Field of Search: 422/81, 422/82.01, 422/82.02, 422/82.05, 422/104, 356/72, 356/73, 356/244, 250/461.2, 209/2, 209/3.1
• US Class Current: 422/82.01
• CPC Class Codes: B01L 1/04 Dust-free rooms or enclosures
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2035/00277 Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)

Abstract

An apparatus and process for measuring particles that prevents the particles from being contaminated during the measuring and optional sorting process wherein a flow chamber or region is mechanically isolated from a sterile enclosure which surrounds the flow chamber. The present invention in one embodiment provides an apparatus comprising of a droplet generator, a charging system, a deflecting system, a sterilization system, a light source, a detector, a cooling system and a vacuum system. The droplet generator and the charging system and the flow chamber associated with the droplet generator operate within an illumination frame where the particles (e.g. biological cells) are actually measured. In order to prevent the biological cells from being contaminated, the illumination frame is located within an isolation chamber which provides an environment that can be sterilized before and after the actual measuring and sorting of the cells, but the illumination frame is mechanically isolated from the isolation chamber. The sterilization system, located outside the isolation chamber sterilizes the isolation chamber before cells are measured and sorted within the isolation chamber, and furthermore, ensures that the isolation chamber is maintained at a pressure above (or alternatively, below) atmospheric pressure during the measuring and sorting of the biological cells. The illumination frame, the light source and the detector are supported by a table to enable the light beam(s) from the light source to intersect the biological cells flowing through the illumination frame, thereby emitting flashes of light (or otherwise effecting the light beam) which are detected by the detector.

Claims

1. A sterile cell cytometric system for measuring at least one property of a sample of cells, said sterile cell cytometric system comprising:

a supporting surface;

an isolation chamber providing an enclosed environment which can be sterilized;

an illumination frame located within said isolation chamber wherein said illumination frame is fixedly attached relative to said supporting surface, said illumination frame supporting a measuring region wherein said at least one property of said sample of cells is measured, wherein said illumination frame is mechanically isolated from said isolation chamber.

2. The sterile system as in claim 1 wherein said illumination frame further comprises a plurality of standoffs inserted through openings in said isolation chamber wherein said plurality of standoffs support said illumination frame, said illumination frame and said isolation chamber being sealed in said openings by a flexible sealant and wherein said at least one property is measured in order to selectively remove certain cells from said sample of cells depending on a measurement of said at least one property.

3. The sterile system as in claim 1 wherein said isolation chamber has a first section and a second section wherein said first section is positioned in front of said second section, said second section is bolted to said supporting surface wherein said supporting surface provides support for said isolation chamber, said first section extends beyond said supporting surface such that said first section overhangs said supporting surface.

4. The sterile system as in claim 1 further comprising a detector table wherein said detector table is supported by said supporting surface, said detector table includes optics for collecting light wherein said optics is located outside said isolation chamber, said detector table further includes an objective lens wherein a portion of said objective lens extends inside said isolation chamber, said portion of said objective lens extending inside said isolation chamber being inserted into a through hole in said illumination frame in order to collect light emitted by said sample of cells.

5. The sterile system as in claim 1 further comprising a light source wherein said light source is supported by said supporting surface, said light source includes at least one light beam source and a corresponding light beam safety pipe wherein said corresponding light beam safety pipe extends inside said isolation chamber and is inserted into a through hole in said illumination chamber thereby providing said illumination frame with at least one beam of light for measurement of said at least one property of said sample of cells.

6. The sterile system as in claim 1 further comprising a sterilization system, said sterilization system is located outside said isolation chamber, said sterilization system sterilizing an area located within said isolation chamber.

7. The sterile system as in claim 1 further comprising a sterilization system,

wherein said sterilization system injects a sterilant into said isolation chamber to sterilize an area located within said isolation chamber by increasing a concentration of said sterilant within said isolation chamber to an effective level, further wherein said sterilization system further recirculates filtered air in said isolation chamber to ventilate said area located within said isolation chamber by reducing said concentration of said sterilant to a negligible level.

8. The sterile system as in claim 1 wherein said isolation chamber includes a panel having at least one glove port having a glove wherein an operator located outside said sterile system may handle objects within said isolation chamber without contaminating said sterile system.

9. The sterile system as in claim 1, wherein said isolation chamber includes a transfer port having a first and a second part linked by a locking mechanism wherein said second part is a canister and wherein objects located outside said isolation chamber may be placed inside said second part and then placed inside said isolation chamber without breaking sterility.

10. The sterile system as in claim 1, wherein said isolation chamber includes a portion of a wall of said isolation chamber and wherein a first laser safety pipe and a second laser safety pipe are optically coupled to said isolation chamber at said portion.

11. The sterile system as in claim 1, wherein said isolation chamber includes a source of pressurized dry gas for drying spills within said isolation chamber.

12. The sterile system as in claim 1, wherein said isolation chamber includes a mesh basket for storing items within said isolation chamber.

13. The sterile system as in claim 11 wherein said isolation chamber includes a first wall and a second wall and wherein a light source provides a first light beam and a second light beam, said first light beam and said second light beam having a first angle between said light beams, said first light beam being directed through said first wall and said second light beam being directed through said second wall, and wherein said first wall and said second wall having a second angle between said walls which substantially equals said first angle.

14. The sterile system as in claim 1 wherein said cells are pluripotent hematopoietic stem cells.

15. The sterile system as in claim 1 wherein said illumination frame supports a droplet generator and wherein said droplet generator is coupled to a cell station which supplies said sample of cells to said droplet generator, wherein said cell station is pressurized with a gas to drive said sample of cells from said cell station to said droplet generator.

16. The sterile system as in claim 15 wherein said isolation chamber includes a seal at a wall of said isolation chamber and wherein a first laser safety pipe and a second laser safety pipe are optically coupled to said isolation chamber at said seal.

17. The sterile system as in claim 1 further comprising electrically controlled valves for controlling flow of fluids and gas pressure in tubes, said tubes being located in said isolation chamber.

18. The sterile system as in claim 12 wherein said valves are pinch valves and wherein sample tubes comprise a tube of delivering said sample of cells.