Biosensor

• US 5,651,869 A
• Filed: 02/22/1996
• Issued: 07/29/1997
• Est. Priority Date: 02/28/1995
• Status: Expired due to Term

• Current Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Ikeda, Shin, Yoshioka, Toshihiko, Nankai, Shiro
• Primary Examiner(s): Gorgos, Kathryn L.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US08/605,391
• Time in Patent Office: 523 Days
• Field of Search: 435/817, 435/14, 204/403
• US Class Current: 204/403.12
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The biosensor of this invention includes an electrically insulating substrate; an electrode system formed on the substrate which includes a working electrode and a counter electrode; and a reaction layer formed on the substrate or above the substrate with a space therebetween. The reaction layer includes a pyranose-oxidizing enzyme.

Claims

1. A biosensor comprising:

an electrically insulating substrate;

an electrode system formed on the substrate which includes a working electrode and a counter electrode; and

a reaction layer formed on the substrate or above the substrate with a space therebetween,

wherein the reaction layer includes a pyranose-oxidizing enzyme and glucose oxidase (EC1.1.3.4).

2. A biosensor according to claim 1, wherein the pyranose-oxidizing enzyme is pyranose oxidase (EC1.1.3.10).

3. A biosensor according to claim 1, wherein the reaction layer further includes an electron acceptor.

4. A biosensor according to claim 3, wherein the electron acceptor is a ferricyanide ion.

5. A biosensor according to claim 4, wherein contents of the pyranose-oxidizing enzyme, the glucose oxidase, and the ferricyanide ion are about 0.1 to about 200 units, 1 to about 200 units, and about 0.21 mg to about 3.30 mg, respectively per 1 cm² of the reaction layer.

6. A biosensor according to claim 1, wherein the reaction layer further includes a polysaccharide hydrolase.

7. A biosensor according to claim 6, wherein the polysaccharide hydrolase is one selected from the group consisting of sucrose hydrolase, maltose hydrolase, and lactose hydrolase.

8. A biosensor according to claim 1, wherein a content of the pyranose-oxidizing enzyme is 1 to about 200 units per 1 cm² of the reaction layer.

9. A biosensor according to claim 1, wherein a content of the pyranose-oxidizing enzyme is about 0.1 to about 200 units per 1 cm² of the reaction layer.

10. A biosensor according to claim 1, wherein the biosensor is used for measuring a blood glucose level.

11. A biosensor for quantitating a biochemical substrate included in a sample liquid by reducing an electron acceptor with an electron generated through an enzyme reaction of the biochemical substrate and electrochemically measuring an amount of a reduced form of the electron acceptor, comprising:

an electrically insulating substrate;

an electrode system formed on the insulating substrate and including a working electrode and a counter electrode; and

a reaction layer formed on the insulating substrate or above the substrate with a space therebetween,

wherein the reaction layer includes a pyranose-oxidizing enzyme, an electron acceptor, and glucose oxidase (EC1.1.3.4).

12. A biosensor according to claim 11, wherein the biosensor is a glucose sensor.

13. A biosensor according to claim 11, wherein the biosensor is used for measuring a blood glucose level.