Ultrasonic contrast agent comprising carbohydrate particles

• US 5,670,135 A
• Filed: 05/22/1995
• Issued: 09/23/1997
• Est. Priority Date: 12/21/1983
• Status: Expired due to Fees

• Current Assignee: GE Healthcare As (GE Healthcare Technologies, Inc.)
• Original Assignee: Nycomed A/S (Takeda Pharmaceutical Company Limited)
• Inventors: Schroder, Ulf
• Primary Examiner(s): Hollinden, Gary E.
• Application Number:
  US08/445,945
• Time in Patent Office: 855 Days
• Field of Search: 424/9.322, 424/646, 424/648, 424/9.5, 424/9.411, 424/9.42, 424/1.29, 514/6, 514/54, 514/56, 514/57, 514/60, 436/173, 128/653.4, 128/654
• US Class Current: 424/9.5
• CPC Class Codes: A61K 2123/00 Preparations for testing in vivo
• CPC Class Codes: A61K 49/0414 Particles, beads, capsules or spheres
• CPC Class Codes: A61K 49/1863 the organic macromolecular compound being a polysaccharide or derivative thereof, e.g. chitosan, chitin, cellulose, pectin, starch
• CPC Class Codes: A61K 49/225 Microparticles, microcapsules gas-filled to be classified in A61K49/223
• CPC Class Codes: A61K 51/1255 Granulates, agglomerates, microspheres
• CPC Class Codes: G01R 33/5601 involving use of a contrast agent for contrast manipulation, e.g. a paramagnetic, super-paramagnetic, ferromagnetic or hyperpolarised contrast agent

Abstract

A method of contrast enhanced ultrasonic diagnostic imaging comprising: administering to a subject a contrast enhancing amount of spheres or particles comprising a matrix enclosing a contrast agent which reflects sound waves, said matrix being a biocompatible, biodegradable, non-immunogenic non-polyamino acid synthetic polymer; and generating an ultrasonic image of said subject.

Claims

1. A method of contrast enhanced ultrasonic diagnostic imaging comprising administering to a subject a contrast enhancing amount of spheres or particles comprising a matrix of a carbohydrate or derivative thereof enclosing a contrast agent which reflects sound waves, and generating an ultrasound image.

2. The method of claim 1 wherein the matrix is of spherical shape.

3. The method of claim 1 wherein the matrix is biocompatible, biologically degradable and non-immunogenic.

4. The method of claim 1 wherein the matrix is stabilized by non-covalent bonds.

5. The method of claim 1 wherein the carbohydrate is endogenous.

6. The method of claim 1 wherein the carbohydrate comprises glucose, maltose, sucrose, sorbitol or dextran.

7. The method of claim 1 wherein the carbohydrate or derivative thereof is a monosaccharide or a polysaccharide having two or more saccharide units.

8. The method of claim 7 wherein the carbohydrate comprises starch, glycogen or dextran.

9. The method of claim 7 wherein the carbohydrate derivative comprises starch crosslinked with acrylates.

10. The method of claim 1 wherein the spheres or particles have a surface structure of hydrophilic character.

11. The method of claim 1 wherein the spheres or particles have a diameter in the range of 0.01-1000 μ

m.

12. The method of claim 1 wherein the spheres or particles have a diameter less than 1 μ

m.

13. The method of claim 12 wherein the particles are built up of conglomerates of smaller particles or spheres.

14. The method of claim 13 wherein the size of the conglomerates is about 10 μ

m.

15. The method of claim 13 wherein the size of the smaller particles or spheres is about 0.5 μ

m.

16. The method of claim 1 wherein an ultrasonic image is generated of the reticuloendothelial system, including the liver and spleen, of said subject.

17. The method of claim 1 comprising the further step of collecting particles to organs by additionally utilizing magnetically responsive particles or spheres and an outer magnetic field.