Planar sensor for determining a chemical parameter of a sample

• US 5,683,562 A
• Filed: 09/14/1995
• Issued: 11/04/1997
• Est. Priority Date: 09/14/1994
• Status: Expired due to Term

• Current Assignee: Avl Medical Instruments AG (Roche Holding AG)
• Original Assignee: Avl Medical Instruments AG (Roche Holding AG)
• Inventors: Ritter, Christoph, Kontschieder, Heinz, Schaffar, Bernhard, Dolezal, Andreas
• Primary Examiner(s): Gorgos, Kathryn L.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US08/528,250
• Time in Patent Office: 782 Days
• Field of Search: 204/400, 204/403, 204/412, 204/282, 427/2.12, 427/2.11, 427/118, 427/102, 427/103, 427/125, 427/126.1, 427/555, 427/231, 427/243, 436/806, 435/217
• US Class Current: 422/82.03
• CPC Class Codes: C12Q 1/001 Enzyme electrodes

Abstract

A planar sensor for determining a chemical parameter of a sample, includes a substrate whose surface is at least partly plane and is provided with at least one potentiometric or amperometric and, possibly, an optical transducer, and one or more biochemical components. The transducer and the biochemical component are provided on the surface of the substrate, or at least part of the surface, as a sensor spot, and a cover membrane surrounding this sensor spot, is heat welded to the surface of the substrate. Those sensor spots that include a potentiometric or amperometric transducer are in contact with a strip conductor attached to the plane surface of the substrate and the thermal seal of the cover membrane is interrupted where the conducting strips lead away from the sensor spots.

Claims

1. Planar sensor for determining a chemical parameter of a sample, comprising a substrate whose surface is at least partly plane and is provided with at least one potentiometric, amperometric or optical transducer, and at least one biochemical component said transducer and said biochemical component, being provided on said surface of said substrate or at least part of said surface as a sensor spot protected by a cover membrane which is gas and ion permeable and is heat welded to the surface of said substrate facing said sample forming a thermal seal, wherein those of said sensor spots that comprise a potentiometric or amperometric transducer are in contact with a strip conductor attached to said plane surface of said substrate, and wherein the thermal seal of said cover membrane is interrupted where said conducting stripes lead away from said sensor spots.

2. Sensor according to claim 1 with an amperometric arrangement, wherein at least one of said sensor spots is configured as a working electrode, and wherein at least one electrode from the group consisting of reference- and counterelectrodes is provided on said surface of said substrate.

3. Sensor according to claim 2, wherein said cover membrane additionally covers said electrode and is heat welded around said electrode to said substrate, the thermal seal being interrupted in an area of strip conductors leading away from said electrode.

4. Sensor according to claim 2, wherein an additional sensor spot with an optical transducer is provided which has an indicator layer with an optical indicator, and at least one layer with said biochemical component.

5. Sensor according to claim 2, wherein an additional sensor spot with a polymer matrix is provided, in or on which an optical indicator and said biochemical component are immobilized either chemically or physically.

6. Sensor according to claim 1 with a potentiometric arrangement, wherein at least one of said sensor spots is configured as a working electrode, and at least one reference electrode is provided on said surface of said substrate.

7. Sensor according to claim 6, wherein an additional sensor spot with an optical transducer is provided which has an indicator layer with an optical indicator, and at least one layer with said biochemical component.

8. Sensor according to claim 6, wherein an additional sensor spot with a polymer matrix is provided, in or on which an optical indicator and said biochemical component are immobilized either chemically or physically.

9. Sensor according to claim 1, wherein at least two sensor spots are provided, a first sensor spot serving as a working electrode and containing an active biochemical component, and a second sensor spot serving as a comparison electrode containing the same biochemical component in a deactivated state.

10. Sensor according to claim 1, wherein said cover membrane is a single layer.

11. Method of producing a planar sensor for determining a chemical parameter of a sample, said method including the steps of:

(a) attaching a strip conductor to at least part of the surface of a substrate,

(b) applying a potentiometric or amperometric transducer layer onto one end of said strip conductor,

(c) affixing at least one biochemical component to said transducer layer,

(d) covering at least said transducer layer and a surrounding area with a single gas and ion permeable cover membrane layer which is heat weldable to said substrate, and

(e) thermally sealing said cover membrane to the substrate in areas not covered by said transducer later and said enzyme layer, the thermal seal being interrupted in the area of said strip conductor.

12. Method according to claim 11, wherein said cover membrane is unwound from a roll of tape, stretched over said surface of the substrate and thermally welded to said substrate by means of a heat stamp.

13. Method according to claim 11, wherein said cover membrane is thermally welded to said substrate by means of a laser technique.

14. Method according to claim 11, wherein said thermal seal obtained in step (e) is continuous.

15. Method according to claim 11, wherein said thermal seal obtained in step (e) is in individual spots.

16. Method of producing a planar sensor for stermining a chemical parameter of a sample, said method including the steps of:

(a) attaching a strip conductor to at least part of the surface of a substrate,

(b) applying a poller matrix on one end of said strip conductor,

(c) immobilizing a potentiometric or amperometric transducer and a biochemical component on or in said polymer matrix,

(d) covering at least aid transducer layer and a surrounding area with a single gas and ion permeable cover membrane layer which is weldable to said substrate, and

(e) thermally sealing said cover membrane to said substrate in areas not covered by said transducer layer and said enzyme layer, the thermal seal being interrupted in the area of said strip conductor.

17. Method according to claim 16, wherein said cover membrane is unwound from a roll of tape, stretched over said surface of the substrate and thermally welded to said substrate by means of a heat stamp.

18. Method according to claim 16, wherein said cover membrane is thermally welded to said substrate by means of a laser technique.

19. Method according to claim 16, wherein said thermal seal obtained in step (e) is continuous.

20. Method according to claim 16, wherein said thermal seal obtained in step (e) is in individual spots.