Diagnosis of autism and treatment therefor

• US 5,686,311 A
• Filed: 06/23/1995
• Issued: 11/11/1997
• Est. Priority Date: 06/23/1995
• Status: Expired due to Term

• Current Assignee: Great Plains Laboratory, Inc.
• Original Assignee: Children's Mercy Hospital
• Inventors: Shaw, William
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/494,200
• Time in Patent Office: 872 Days
• Field of Search: 436/86, 436/91, 436/98, 436/811
• US Class Current: 436/86
• CPC Class Codes: G01N 30/7206 interfaced to gas chromatograph interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04
• CPC Class Codes: G01N 33/70 involving creatine or creatinine
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function
• CPC Class Codes: Y10T 436/14 Heterocyclic carbon compound [i.e. , O, S, N, Se, Te, as only ring hetero atom]
• CPC Class Codes: Y10T 436/147777 Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.]

Abstract

A method for diagnosing the likelihood of autism in patients is provided which comprises first obtaining from the patient a sample of body fluid such as urine and analyzing the sample to determine the quantity therein of at least one marker compound selected from the group consisting of citramalic acid, 5-hydroxy-methyl-2-furoic acid, 3-oxo-glutaric acid, furan-2,5-dicarboxylic acid, tartaric acid, furancarbonylglycine, arabinose, dihydroxyphenylpropionic acid, carboxycitric acid and phenylcarboxylic acid; if the quantities of one or more of the compounds are abnormally high, as compared with the urine of non-autistic individuals, an ultimate diagnosis of autism is likely. The invention also pertains to a method of treating autistic patients by administration of antifungal drugs, in order to ameliorate the clinical symptoms of autism.

Claims

1. A method of detecting autism-related compounds in a patient comprising the steps of:

obtaining from the patient a sample of a body fluid selected from the group consisting of urine, blood, saliva and cerebral spinal fluid;

analyzing said sample to determine the quantity therein of at least one compound selected from the group consisting of citramalic acid, 5-hydroxy-methyl-2-furoic acid, 3-oxo-glutaric acid, furan-2,5-dicarboxylic acid, tartaric acid, furancarbonylglycine, arabinose, dihydroxyphenylpropionic acid, carboxycitric acid and phenylcarboxylic acid; and

correlating the quantity of said at least one compound with an autism condition or lack thereof in said patient.

2. The method of claim 1, including the step of determining if said quantity is abnormally high as compared with a normal quantity thereof found in the same type of body fluid from non-autistic individuals of approximately the same age.

3. The method of claim 1, said body fluid being urine.

4. The method of claim 3, including the step of determining if said quantity is abnormally high as compared with a normal quantity thereof found in urine from non-autistic individuals of the same age, the quantity of said compounds being abnormal if present in the urine sample in at least the following amounts, respectively:

(a) at least about 10 mmol citramalic acid/mol creatinine in said urine sample;

(b) at least about 100 mmol 5-hydroxy-methyl-2-furoic acid/mol creatinine in said urine sample;

(c) at least about 300 mmol arabinose/mol creatinine in said urine sample;

(d) at least about 100 mmol furan-2,5-dicarboxylic acid/mol creatinine in said urine sample

(e) at least about 90 mmol tartaric acid/mol creatinine in said urine sample;

(f) at least about 100 mmol furancarbonylglycine/mol creatinine in said urine sample;

(g) at least about 1 mmol 3-oxo-glutaric acid/mol creatinine in said urine sample;

(h) at least about 250 mmol dihydroxyphenylpropionic acid/mol creatinine in said urine sample;

(i) at least about 50 mmol carboxycitdc acid/mol creatinine in said urine sample; and

(j) at least about 100 mmol phenycarboxylic acid/mol creatinine in said urine sample.

5. The method of claim 1, including the step of analyzing said sample to determine the quantities of a plurality of said compounds therein.

6. The method of claim 1, including the step of collecting a plurality of said samples over a period of time.

7. The method of claim 6, said sample being a urine sample, and including the step of collecting individual urine samples on a daily basis over a period of at least seven days.