Method for the screening of familial hemiplegic migraine (FHM)

• US 5,714,319 A
• Filed: 02/28/1994
• Issued: 02/03/1998
• Est. Priority Date: 02/28/1994
• Status: Expired due to Term

• Current Assignee: L'Assistance Publique - Hopitaux De Paris
• Original Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH)
• Inventors: Bousser, Marie-Germaine Madeleine, Joutel, Anne Marie Giseple, Tournier-Lasserve, Elisabeth Andree
• Primary Examiner(s): Campbell, Eggerton A.
• Application Number:
  US08/203,194
• Time in Patent Office: 1,436 Days
• Field of Search: 435/7, 435/91.2, 935/77, 935/78
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/172 Haplotypes

Abstract

Method for screening for familial hemiplegic migraine which comprises searching for the presence of a mutated gene responsible for familial hemiplegic migraine on the chromosome 19, in the region comprised between the microsatellites D19S216 and D19S215, of a family or of an at risk individual, including fetus.

Claims

1. Method for screening for familial hemiplegic migraine which comprises searching for the presence of genetic abnormalities via polymorphic analysis linked to familial hemiplegic migraine on the chromosome 19, in the region comprised between the microsatellites D19S216 and D19S215, of a family or of an at risk individual, including fetus.

2. The method according to claim 1, wherein the microsatellites used are chosen among the group consisting of:

D19S216, D19S76, D19S221, D19S179, D19S226, D19S252, D19S253, D19S244, D19S415, D19S199, D19S215.

3. A method for screening for familial hemiplegic migraine which comprises selecting polymorphisms via linkage analysis within microsatellite regions responsible for familial hemiplegic migraine on chromosome 19 with the aid of DNA probes to a linkage analysis with the microsatellites D19S216, D19S76, D19S221, D19S179, D19S226, D19S252, D19S253, D19S244, D19S415, D19S199 and D19S215.

4. Method for screening for a subgroup of familial hemiplegic migraine linked to chromosome 19 by a family or an at risk individual, including a fetus, comprising the step of searching for a genetic linkage between DNA polymorphisms and a mutated region linked to the familial hemiplegic migraine, these DNA polymorphisms being located in the region of the chromosome 19 comprised between the microsatellites D19S216 and D19S215, including these microsatellites.

5. The method according to claim 4, wherein the method is first applied on members of a family whose status for familial hemiplegic migraine is known, including ill and healthy members, comprising the steps of determining if the family is linked to abnormalities on chromosome 19, determining the DNA polymorphisms which are the best informatives for the family, choosing primer pairs to the above-determined DNA polymorphisms, amplifying DNA fragments from an at risk individual to be tested and pertaining to this family with the selected primer pairs, and determining if this at risk individual is carrying the affected or healthy polymorphisms and calculating the risk of this individual having inherited the affected region.