Strip electrode with screen printing
First Claim
1. A method of making a single use disposable electrode strip for attachment to signal readout circuitry of a sensor system to detect a current representative of a compound in a liquid sample, the strip comprising:
- a) an elongated support adapted for releasable attachment to said readout circuitry;
b) a first conductor extending along said support and comprising a conductive element for connection to said readout circuitry; and
c) an active electrode on said support in contact with said first conductor, said active electrode comprising, in a single printed layer on said support, an admixture of an enzyme capable of catalyzing a reaction involving a substrate for said enzyme, a conductive material, and a mediator capable of transferring electrons transferred between said enzyme-catalyzed reaction and said first conductor to create a current representative of the activity of said enzyme and representative of said compound;
the method comprising;
a) supplying an ink comprising said admixture of said enzyme, said conductive material, and said mediator; and
b) printing said active electrode by applying said ink to said support in a single layer in contact with said first conductor.
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Abstract
A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a single layer deposit of an enzyme capable of catalyzing a reaction involving the compound, a conductive material, and an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing, an admixture of an enzyme a conductive material, and a mediator as a single layer onto the substrate.
639 Citations
8 Claims
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1. A method of making a single use disposable electrode strip for attachment to signal readout circuitry of a sensor system to detect a current representative of a compound in a liquid sample, the strip comprising:
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a) an elongated support adapted for releasable attachment to said readout circuitry; b) a first conductor extending along said support and comprising a conductive element for connection to said readout circuitry; and c) an active electrode on said support in contact with said first conductor, said active electrode comprising, in a single printed layer on said support, an admixture of an enzyme capable of catalyzing a reaction involving a substrate for said enzyme, a conductive material, and a mediator capable of transferring electrons transferred between said enzyme-catalyzed reaction and said first conductor to create a current representative of the activity of said enzyme and representative of said compound; the method comprising; a) supplying an ink comprising said admixture of said enzyme, said conductive material, and said mediator; and b) printing said active electrode by applying said ink to said support in a single layer in contact with said first conductor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification