Chimaeric gene coding for a transit peptide and a heterologous polypeptide
DC CAFCFirst Claim
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1. A plant containing in its nuclear genome a chimaeric DNA sequence, comprising:
- a) a nucleic acid sequence coding for a chimaeric protein or polypeptide comprising in sequence;
1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or chloroplast polypeptide of a plant species, and2) a protein or polypeptide of interest which is different from a mature chloroplast protein or chloroplast polypeptide derived from a natural cytoplasmic precursor thereof, and,b) a promoter upstream of said nucleic acid sequence recognized by polymerases endogenous to said plant,wherein the chimaeric DNA sequence can be expressed in cells of said plant under the control of said promoter, and said protein or polypeptide of interest is translocated into the chloroplasts of said cells of said plant.
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Abstract
Chimaeric DNA sequence which encodes: 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or polypeptide of a plant and 2) a protein or polypeptide that is heterologous to the transit peptide. The chimaeric DNA sequence can be used as a vector for transforming a plant cell so that a chimaeric precursor of the heterologous protein or polypeptide is produced in the cytoplasm of the cell and the chimaeric precursor then transports the heterologous protein or polypeptide in vivo into a chloroplast of the cell.
585 Citations
43 Claims
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1. A plant containing in its nuclear genome a chimaeric DNA sequence, comprising:
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a) a nucleic acid sequence coding for a chimaeric protein or polypeptide comprising in sequence; 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or chloroplast polypeptide of a plant species, and 2) a protein or polypeptide of interest which is different from a mature chloroplast protein or chloroplast polypeptide derived from a natural cytoplasmic precursor thereof, and, b) a promoter upstream of said nucleic acid sequence recognized by polymerases endogenous to said plant, wherein the chimaeric DNA sequence can be expressed in cells of said plant under the control of said promoter, and said protein or polypeptide of interest is translocated into the chloroplasts of said cells of said plant. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 42, 43)
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14. A plant containing in its nuclear genome a chimaeric DNA sequence comprising:
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a) a nucleic acid sequence coding for a chimaeric protein or polypeptide comprising in sequence; 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or chloroplast polypeptide of a plant species, and 2) a hybrid protein comprising in sequence; 2.1) a linker, and 2.2) a protein or polypeptide of interest which is different from said chloroplast protein or chloroplast polypeptide wherein said linker does not significantly alter the biological property of said protein or polypeptide of interest in said hybrid protein, and b) a promoter upstream of said nucleic acid sequence recognized by polymerases endogenous to a plant, wherein the chimaeric DNA sequence is expressed in cells of said plant under the control of said promoter, and said hybrid protein is translocated into the chloroplasts of said cells of the plant. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A plant containing in its nuclear genome a chimaeric DNA sequence comprising:
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a) a nucleic acid sequence coding for a chimaeric protein or polypeptide comprising in sequence; 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or chloroplast polypeptide of a plant species, and, 2) a fusion protein comprising in sequence, 2.1) a N-terminal part consistinf of no more than the first 22 N-terminal amino acids of a mature chloroplast protein or chloroplast polypeptide derived from a natural cytoplasmic precursor thereof, and 2.2) a protein or polypeptide of interest which is different from said chloroplast protein or polypeptide and, b) a promoter upstream of said nucleic acid sequence recognized by polymerases encodenous to said plant, wherein the chimaeric DNA sequence is expressed in cells of said plant under the control of said promoter, and said fusion protein is translocated into the chloroplasts of said cells of said plant. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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Specification