Chimaeric gene coding for a transit peptide and a heterologous polypeptide

CAFC
  • US 5,728,925 A
  • Filed: 04/28/1995
  • Issued: 03/17/1998
  • Est. Priority Date: 12/28/1984
  • Status: Expired due to Term
First Claim
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1. A plant containing in its nuclear genome a chimaeric DNA sequence, comprising:

  • a) a nucleic acid sequence coding for a chimaeric protein or polypeptide comprising in sequence;

    1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or chloroplast polypeptide of a plant species, and2) a protein or polypeptide of interest which is different from a mature chloroplast protein or chloroplast polypeptide derived from a natural cytoplasmic precursor thereof, and,b) a promoter upstream of said nucleic acid sequence recognized by polymerases endogenous to said plant,wherein the chimaeric DNA sequence can be expressed in cells of said plant under the control of said promoter, and said protein or polypeptide of interest is translocated into the chloroplasts of said cells of said plant.

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