Flow detection appartus and method

• US 5,736,404 A
• Filed: 12/27/1995
• Issued: 04/07/1998
• Est. Priority Date: 12/27/1995
• Status: Expired due to Fees

• Current Assignee: Zia Yassinzadeh
• Original Assignee: Zia Yassinzadeh
• Inventors: Lingane, Paul J., Yassinzadeh, Zia
• Primary Examiner(s): Le, Long V.
• Application Number:
  US08/579,367
• Time in Patent Office: 832 Days
• Field of Search: 436/43, 436/44, 436/46, 436/49, 436/52, 436/54, 436/63, 436/69, 436/174, 436/179, 436/180, 436/164, 436/165, 422/57, 422/58, 422/63, 422/68.1, 422/73, 422/81, 422/82.01, 422/82.02, 422/82.05, 422/100, 422/102
• US Class Current: 436/52
• CPC Class Codes: G01N 11/04 through a restricted passage, e.g. tube, aperture
• CPC Class Codes: G01N 2035/00425 Heating or cooling means associated with pipettes or the like, e.g. for supplying sample/reagent at given temperature
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/54
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25625 Dilution

Abstract

Fluid sample tester assembly (12) comprises a cartridge (6) having a pressure chamber (22) and entrance port (20) connected by a passageway (24). An analyte reagent (36) is positioned along the fluid passageway so that when a liquid sample (28) is drawn through the entrance port into the passageway, the analyte reagent mixes with the sample. The sample is preferably drawn into the cartridge by temporarily reducing the volume of the pressure chamber, applying the sample to the entrance port and then returning the pressure chamber to its initial volume; this can also be done by heating the chamber, contacting the sample and then cooling the chamber. The end of the sample defines a boundary surface (30) along the fluid passageway. Positive and negative pressure is applied to the sample to cause the boundary surface to oscillate within the passageway. The position of the boundary surface is continuously monitored so that continuous boundary position data is obtained and is analyzed to obtain a flow-related characteristic, typically speed of coagulation, of the sample.

Claims

1. A method for determining coagulation time of a blood sample comprising the following steps:

drawing a blood sample into a fluid passageway, the blood sample having a boundary surface along said passageway;

applying positive and negative pressures to the blood sample by varying the volume in a pressure chamber fluidly coupled to said passageway to reciprocate the boundary surface of the blood sample back and forth along said fluid passageway;

initiating blood coagulation;

continuously monitoring the location of the boundary surface to obtain continuous boundary position data; and

analyzing the boundary position data to determine the blood coagulation time.

2. The method according to claim 1 wherein the initiating step comprises mixing the blood sample with a coagulation reagent.

3. The method according to claim 2 wherein the mixing step is carried out with the coagulation reagent carried on a wall of the fluid passageway.

4. The method according to claim 1 wherein the sample drawing step includes the step of cooling a gas-filled thermal pressure chamber, fluidly coupled to the fluid passageway, to reduce the density of the gas within the thermal pressure chamber.

5. The method according to claim 1 wherein the pressure applying step is carried out by mechanically reciprocating a movable element bounding a fluid-filled pressure chamber, the pressure chamber fluidly coupled to the fluid passageway thereby changing the volume of the pressure chamber.

6. The method according to claim 5 wherein the pressure applying step is carried out using a gas-filled thermal pressure chamber fluidly coupled to the fluid passageway and includes the step of alternately heating and cooling the gas within the fluid pressure chamber.

7. The method according to claim 1 wherein the continuously monitoring step is carried out using an optical sensor.

8. The method according to claim 1 wherein the continuously monitoring step comprises monitoring the magnitude and phase of the output of an optical sensor which is oriented transverse to the fluid passageway.

9. The method according to claim 1 wherein the continuously monitoring step is carried out using at least one of the following:

a capacitance sensor, a conductance sensor, an optical sensor and an acoustic sensor.