Liposome compositions and kits therefor

• US 5,738,868 A
• Filed: 07/18/1995
• Issued: 04/14/1998
• Est. Priority Date: 07/18/1995
• Status: Expired due to Fees

• Current Assignee: Lipogenics Ltd.
• Original Assignee: Lipogenics Ltd.
• Inventors: Shinkarenko, Leonid Lurya
• Primary Examiner(s): Kishore, Gollamudi S.
• Application Number:
  US08/503,662
• Time in Patent Office: 1,001 Days
• Field of Search: 424/450, 424/1.21, 424/9.321, 264/4.1, 264/4.3, 264/4.6
• US Class Current: 424/450
• CPC Class Codes: A61K 51/1234 Liposomes

Abstract

A method for preparing a radio labelled liposome, the method including the steps of at least once dehydrating and rehydrating a treated liposome to form a binding liposome, and adding a radio label to said binding liposome to form the radio labelled liposome.

Claims

1. A method for preparing a radio labelled liposome, the method comprising the steps of:

(a) adding a reducing agent to a liposome under vacuum to form a treated liposome;

(b) dehydrating under vacuum said treated liposome, to form a dehydrated liposome;

rehydrating under vacuum said dehydrated liposome, to form a rehydrated liposome; and

(d) adding a radio label to said rehydrated liposome under vacuum, to form a radio labelled liposome binding at least about 90% of an added radio label.

2. A method for preparing a radio labelled liposome, the method comprising the steps of:

(a) adding a reducing agent to a liposome under vacuum to form a treated liposome;

(b) dehydrating said treated liposome under vacuum, to form a dehydrated liposome;

(c) rehydrating said dehydrated liposome under vacuum, to form a rehydrated liposome; and

(d) adding a radio label to said rehydrated lipsome under vacuum, to form the radio labelled liposome.

3. The method of claim 2, wherein said liposome is contained in a liposome suspension.

5. The method of claim 2, wherein said steps of dehydrating and rehydrating are repeated prior to said step of adding said radio label.

6. The method of claim 5, wherein said step of dehydrating includes lyophilization.

7. The method of claim 2, wherein said reducing agent is stannous chloride.

8. The method of claim 2, wherein said liposome is formed from an ampiphile selected from the group consisting of dilauryl phosphatidyl choline, dimyristol phosphatidyl choline, dipalmitoyl phosphatidyl choline, dioleoyl phosphatidylcholine and distereoyl phosphatidyl choline.

4. A method for preparing a radio labelled liposome, the method comprising the steps of:

(a) adding stannous chloride to a liposome under vacuum to form a treated liposome;

(b) dehydrating said treated liposome under vacuum, to form a dehydrated liposome;

(c) rehydrating said dehydrated liposome under vacuum, to form a rehydrated liposome; and

(d) adding a radio label to said rehydrated lipsome under vacuum, to form the radio labelled liposome.