AHAS promoter useful for expression of introduced genes in plants
1. An isolated nucleotide sequence comprising a maize AHAS promoter capable of constitutive expression.
The present invention is directed to isolated non-coding nucleotide sequences useful as promoters for heterologous gene expression in plants. The present invention is also directed to vectors and plant cells comprising the isolated nucleotide sequences.
AHAS promoters from maize are used to express introduced genes at high levels and in various plant tissues. Promoters from the als1 and als2 genes of maize are cloned and sequenced, and the promoter regions from these genes are then introduced into a plasmid 5'"'"' to the reporter gene beta-glucuronidase (GUS). Both promoter fragments are from the XI12 maize line. The als1 promoter fragment is approximately 1400 base pairs long, whereas the als2 promoter fragment contains 829 base pairs.
- 1. An isolated nucleotide sequence comprising a maize AHAS promoter capable of constitutive expression.