Methods and apparatus using hydrogels

• US 5,770,086 A
• Filed: 01/25/1996
• Issued: 06/23/1998
• Est. Priority Date: 01/25/1996
• Status: Expired due to Fees

• Current Assignee: Eureka! Science Corp.
• Original Assignee: Eureka| Science Corporation
• Inventors: Andrews, Patricia C., Indriksons, Andris
• Primary Examiner(s): Drodge, Joseph W.
• Application Number:
  US08/591,139
• Time in Patent Office: 880 Days
• Field of Search: 210/321.87, 210/650, 210/642, 210/644, 210/321.6, 210/645, 210/502.1, 210/490, 210/500.35, 210/635, 210/643, 436/514, 436/515, 436/535, 436/824, 436/517, 436/518, 422/101, 422/102, 435/7.1
• US Class Current: 210/643
• CPC Class Codes: B01D 61/00 Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor separation of gases or vapours by diffusion B01D53/22
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 436/824 Immunological separation techniques

Abstract

The hydrogel-forming polymers are used as absorbents for collecting and concentrating solutions. A solution to be concentrated can be contained in a dialysis membrane, with the hydrogel-forming polymer, or mixture thereof, on the outer surface of the membrane. A hydrogel-forming polymer can be added directly to the solution to be concentrated and the resulting concentrate separated from the gel by filtration of decanting. A hydrogel-forming polymer can be placed in a container formed of porous material and the container can be added to the solution to be concentrated and physically removed after concentration has occurred. Hydrogel-forming polymer can be used as a liquid collection in diagnostic membrane assay cartridges.

Claims

1. A method of concentrating a solution, comprising

contacting the solution with one side of a porous gel barrier material and contacting the other side of the porous gel barrier material with a substance including a water-insoluble hydrogel forming polymer, and

allowing liquid to pass through the porous gel barrier material to concentrate the solution.

2. The method of claim 1 wherein the other side of the porous gel barrier is concentrated with a mixture including said water-insoluble hydrogel-forming polymer.

3. A method of obtaining a desired concentration of a suspension including particulates and a solution, comprising

placing a water-insoluble hydrogel-forming polymer in contact with the suspension and allowing the hydrogel-forming polymer to absorb the solution until the desired concentration is obtained, and

separating the hydrogel-forming polymer and absorbed solution from the desired concentration of solution and particulates to provide the desired concentration of the suspension for further use.

4. A method of concentrating a solution or suspension, comprising

placing a water-insoluble hydrogel-forming polymer in a container formed from a porous gel barrier material,

placing the container of water-insoluble hydrogel-forming polymer into the solution,

allowing liquid to pass through the porous gel barrier material for absorption by the water-insoluble hydrogel-forming polymer to concentrate the solution or suspension, and removing the container of water-insoluble hydrogel-forming polymer and absorbed liquid from the container of concentrated solution or suspension.

5. The method of claim 4 wherein an appendage is attached to the container to facilitate its removal.

6. The method of claim 4 wherein the porous gel barrier material is formed to provide an expandable container.

7. The method of claim 4 wherein the container has a solution or suspension contacting surface with an area in the range of about 0.1 to about 10.0 cm² /ml of solution.

8. The method of claim 7 wherein the solution contacting area of the container is about 1.5 cm² /ml of solution.

9. The method of claim 4 wherein the container comprises a bag-like container formed from a fibrous material having a pore size small enough to contain particulate matter as small as one micron, and the water-insoluble hydrogel-forming polymer is contained within the bag-like container.

10. A method of concentrating a suspension containing particulates and a solution, comprising

placing the suspension to be concentrated into a container formed with a semi-permeable membrane having pores smaller than the particulates,

placing the container in contact with a water-insoluble hydrogel-forming polymer for a time sufficient to allow solution to be absorbed by the water-insoluble hydrogel-forming polymer thereby concentrating the suspension.

11. A method of concentrating a solute-containing solution by direct addition of a water-insoluble hydrogel-forming polymer to the solution to be concentrated and subsequent removal of the water insoluble hydrogel-forming polymer from the concentrated solution upon obtaining a desired concentration so that the concentrated solution is available for further use.

12. A method of concentrating a suspension by direct addition of a water-insoluble hydrogel-forming polymer to the suspension to be concentrated, and subsequent removal of the water insoluble hydrogel-forming polymer from the concentrated suspension upon obtaining a desired concentration so that the concentrated solution is available for further use.

13. In a cartridge for conducting immunoassay procedures including a porous membrane carrying a member of an antigen/antibody pair of interest and providing a test detection site and means for absorbing from the porous membrane a liquid sample to be tested for the other member of the antigen/antibody pair of interest, the improvement comprising a water-insoluble hydrogel-forming polymer supported in contact with the porous membrane and providing said means to absorb said liquid sample from the porous membrane.

14. The cartridge of claim 13 wherein the hydrogel-forming polymer is supported in contact with the porous membrane by a collapsible support contained within a lower portion of the cartridge.