Preparation of three-dimensional fibrous scaffold for attaching cells to produce vascularized tissue in vivo

  • US 5,770,193 A
  • Filed: 02/28/1994
  • Issued: 06/23/1998
  • Est. Priority Date: 11/20/1986
  • Status: Expired due to Term
First Claim
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1. A method for preparing a scaffold for preparing a cell-scaffold composition for growing cells in vitro to produce functional vascularized organ tissue in vivo comprising:

  • providing fibers made of a biocompatible, biodegradable, synthetic polymer, and forming the fibers into a fibrous three-dimensional scaffold composed of the fibers,wherein the fibers of the scaffold provide sufficient surface area to permit attachment in vitro of an amount of the cells effective to produce the functional vascularized organ tissue in vivo;

    wherein the fibers of the scaffold are spaced apart such that the maximum distance over which diffusion of nutrients and gases must occur through a mass of cells attached to the fibers is between 200 and 300 microns; and

    wherein the diffusion provides free exchange of nutrients, gases and waste to and from the cells uniformly attached to the fibers of the scaffold and proliferating throughout the scaffold in an amount effective to maintain cell viability throughout the scaffold in the absence of vascularization.

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