High performance liquid chromatography method and apparatus

  • US 5,772,874 A
  • Filed: 06/11/1996
  • Issued: 06/30/1998
  • Est. Priority Date: 11/02/1995
  • Status: Expired due to Term
First Claim
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1. Chromatography apparatus comprising, in combination,a chromatographic body formed as a substantially uniformly distributed multiplicity of rigid, solid, porous particles with chromatographically active surfaces, said particles having average diameters of greater than about 30 μ

  • m, the interstitial volume between said particles being not less than about 45% of the total volume of said column; and

    means for loading said surfaces with at least one solute that is reactive with said surfaces, by flowing a liquid mixture containing said solute through said body at a velocity sufficient to induce flow of said mixture within at least a substantial portion of said interstitial volume at a reduced velocity greater than about 5,000.

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