High performance liquid chromatography method and apparatus

• US 5,772,874 A
• Filed: 06/11/1996
• Issued: 06/30/1998
• Est. Priority Date: 11/02/1995
• Status: Expired due to Term

• Current Assignee: Cohesive Technologies Incorporated (Thermo Fisher Scientific Incorporated)
• Original Assignee: Cohesive Technologies Incorporated (Thermo Fisher Scientific Incorporated)
• Inventors: Takarewski, Joseph J. Jr., Quinn, Hubert M.
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US08/661,367
• Time in Patent Office: 749 Days
• Field of Search: 210/198.2, 210/502.1, 210/635, 210/656, 210/659
• US Class Current: 210/198.2
• CPC Class Codes: B01D 15/14 relating to the introduction of the feed to the apparatus
• CPC Class Codes: B01D 15/16 relating to the conditioning of the fluid carrier
• CPC Class Codes: B01J 20/06 comprising oxides or hydroxides of metals not provided for in group B01J20/04
• CPC Class Codes: B01J 20/08 comprising aluminium oxide or hydroxide; comprising bauxite
• CPC Class Codes: B01J 20/103 comprising silica
• CPC Class Codes: B01J 20/20 comprising free carbon; comprising carbon obtained by carbonising processes
• CPC Class Codes: B01J 20/28004 Sorbent size or size distribution, e.g. particle size
• CPC Class Codes: B01J 20/28016 Particle form
• CPC Class Codes: B01J 20/28019 Spherical, ellipsoidal or cylindrical
• CPC Class Codes: B01J 20/28083 being in the range 2-50 nm, i.e. mesopores
• CPC Class Codes: B01J 20/28085 being more than 50 nm, i.e. macropores
• CPC Class Codes: B01J 20/282 Porous sorbents ion exchange B01J39/00 - B01J41/00
• CPC Class Codes: B01J 20/283 based on silica
• CPC Class Codes: B01J 20/284 based on alumina
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: G01N 2030/324 speed, flow rate
• CPC Class Codes: G01N 2030/387 Turbulent flow of mobile phase
• CPC Class Codes: G01N 30/38 Flow patterns
• CPC Class Codes: G01N 30/50 Conditioning of the sorbent material or stationary liquid

Abstract

A method of and apparatus for liquid chromatography at improved analytical and preparative speeds and quantities involves flow of fluids through the column at flow rates sufficient to induce turbulent flow in those fluids. In one embodiment, the apparatus and method includes a substantially uniform chromatography column created by packing together a multiplicity of rigid, solid, porous particles having diameters of not less than about 30 μm, surfaces of the particles being chromatographically active. A fluid mixture containing at least one solute that is reactive with the particle surfaces is injected into the column and subsequently eluted therefrom by a eluant fluid flow, both the injection and elution being effected at a velocity sufficient to induce flow within at least a substantial portion of the interstitial volume between the particles at a reduced velocity greater than about 5,000.

Claims

1. Chromatography apparatus comprising, in combination,

a chromatographic body formed as a substantially uniformly distributed multiplicity of rigid, solid, porous particles with chromatographically active surfaces, said particles having average diameters of greater than about 30 μ

m, the interstitial volume between said particles being not less than about 45% of the total volume of said column; and

means for loading said surfaces with at least one solute that is reactive with said surfaces, by flowing a liquid mixture containing said solute through said body at a velocity sufficient to induce flow of said mixture within at least a substantial portion of said interstitial volume at a reduced velocity greater than about 5,000.

2. Chromatography apparatus as set forth in claim 1 wherein said mean diameters of said particles are substantially uniform.

3. Chromatography apparatus as set forth in claim 1 wherein said interstitial volume comprises a multiplicity of interstitial channels between said particles, at least the majority of said channels having mean cross-section dimensions substantially not less than about 5 μ

m.

4. Chromatography apparatus as set forth in claim 1 wherein said particles are coated with stationary phase layers so as to render said surfaces chromatographically active.

5. Chromatography apparatus as set forth in claim 1 wherein said particles are substantially incompressible so as to substantially resist plastic deformation at packing pressures up to about 1×

10⁴ psi.

6. Chromatography apparatus as set forth in claim 1 wherein said particles are formed from one or more of alumina, silica, titania, vanadia, zirconia and carbon.

7. Chromatography apparatus as set forth in claim 1 wherein the pores in said particles have mean diameters lying within the range of from about 60 Å

to about 5,000 Å

.

8. Chromatography apparatus as set forth in claim 1 wherein said particles are packed together under a pressure of substantially not less than 5×

10³ psi.

9. Chromatography apparatus as set forth in claim 1 including means for eluting loaded solute from said body by flowing an eluant fluid through said body at a velocity sufficient to induce flow of said eluant fluid within at least a substantial portion of said interstitial volume at a reduced velocity greater than about 5,000.

10. Chromatography apparatus as set forth in claim 1 including means for eluting loaded solute from said body by flowing an eluant fluid through said interstitial volume at a velocity selected such that band spreading of solute eluted by said eluant fluid from said body is an inverse function of the Reynolds number for the eluant fluid flow and is a direct function of the magnitude of the diffusion coefficient of said solute in said eluant fluid.

11. Chromatography apparatus as set forth in claim 1 including means for eluting loaded solute from said flowing an eluant fluid through said interstitial volume at a velocity selected such that band spreading of solute eluted by said eluant fluid from said body decreases with increasing Reynolds number of the eluant flow.

12. Chromatography apparatus as set forth in claim 1 including means for eluting loaded solute rom said body by flowing eluant fluid through said interstitial volume at a velocity selected such that band spreading of solute eluted by said eluant fluid from said body is a direct function of the magnitude of the diffusion coefficient of said solute in said eluant fluid.

13. Chromatography apparatus comprising, in combination,

a chromatographic column formed as a substantially uniformly distributed, packed multiplicity of rigid, solid, porous particles with chromatographically active surfaces, said particle having substantially uniform average diameters of not less than about 500 μ

m, and

means for loading said surfaces with at least one solute that is reactive with said surfaces, by flowing a liquid mixture containing said solute through said column at a velocity sufficient to induce flow of said mixture within at least a substantial portion of the interstitial volume between said particles at a reduced velocity greater than about 50,000.

14. Chromatography apparatus as set forth in claim 13 including means for eluting loaded solute from said column by flowing an eluant fluid through said column at a reduced velocity value of at least about 50,000.

15. In chromatography apparatus including a chromatographic column formed as a bed of a packed multiplicity of rigid solid particles having substantially uniform mean diameters of not less than about 30 μ

m, the surfaces of said particles being chromatographically active and wherein a solute introduced into said bed tends to become non-specifically bound to said particles, the improvement including

means for injecting a liquid mixture comprising said solute into said column at a reduced velocity greater than about 5,000 so as to load said particles with said solute; and

means for eluting the loaded solute from said particles by flowing eluant fluid through said column at an average reduced velocity greater than about 5000.

16. A chromatographic column comprising, in combination,

a container packed with a substantially uniformly distributed multiplicity of rigid, solid, porous particles with chromatographically active surfaces, said particle having substantially uniform average diameters in the range between about 30 to about 500 μ

m, the interstitial volume between said particles being greater than about 45% of the total volume of said body, said volume being formed of a multiplicity of interstitial channels between said particles, at least a majority of said channels having mean cross-section dimensions substantially not less than about 5 μ

m.

17. A chromatographic column as set forth in claim 16 wherein said particles are coated with stationary phase layers so as to render said surfaces chromatographically active.

18. A chromatographic column as set forth in claim 16 wherein said particles are substantially incompressible so as to substantially resist plastic deformation at packing pressures up to about 1×

10⁴ psi.

19. A chromatographic column as set forth in claim 16 wherein said particles are formed from one or more of alumina, silica, titania, vanadia, zirconia and carbon.

20. A chromatographic column as set forth in claim 16 wherein the pores in said particles have mean diameters lying within the range of from about 60 Å

to about 5,000 Å

.

21. A chromatographic column as set forth in claim 16 wherein said particles are packed together under a pressure of substantially not less than 5×

10³ psi.

22. A chromatographic column apparatus as set forth in claim 16 wherein said particles are selected to have diameters of at least about 500 μ

m.