Inner supported biocompatible cell capsules

• US 5,773,286 A
• Filed: 05/24/1995
• Issued: 06/30/1998
• Est. Priority Date: 11/17/1987
• Status: Expired due to Fees

• Current Assignee: Neurotech USA, Inc. (Woodbrook Group Holdings, Inc.)
• Original Assignee: Cytotherapeutics, Inc.
• Inventors: Flanagan, Thomas R., Dionne, Keith E., Doherty, Edward J., Hegre, Orion D., Hazlett, Tyrone F.
• Primary Examiner(s): Naff, David M.
• Application Number:
  US08/449,568
• Time in Patent Office: 1,133 Days
• Field of Search: 435/174, 435/177, 435/180, 435/182, 435/188, 435/240.22, 435/240.23, 435/240.242, 435/297.1, 435/395, 435/382, 435/289.1, 604/890.1, 424/93.7
• US Class Current: 435/297.1
• CPC Class Codes: A61F 2/022 Artificial gland structures using bioreactors hemodialysis A61M1/16; hemofiltration A61M1/34; other extracorporeal blood treatment A61M1/36
• CPC Class Codes: A61K 2035/128 capsules, e.g. microcapsules
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: B01J 13/02 Making microcapsules or microballoons for medical preparations A61K9/50
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0012 Cell encapsulation
• CPC Class Codes: C12N 5/0614 Adrenal gland

Abstract

A biocompatible capsule for containing cells for implantation is prepared containing an inner support that provides tensile strength to the capsule. Cells within the capsule are suspended in a liquid medium or immobilized in a hydrogel or extracellular matrix material, and are surrounded by a semipermeable membrane across which biologically active molecules can be delivered from the capsule to surroundings or from the surroundings into the capsule. The inner support may be formed as an integral part of the capsule during a coextrusion process to form the capsule. Alternatively, the inner support may be a discrete component within a tube having top and bottom sealing fittings that are linked withing the tube by the support. The inner support may have external features such as flutes or a roughened or irregularly-shaped surface, and may be coated with cell-adhesive substance or a cell-viability-enhancing substance. The inner support may be a hollow tube having two channels, one communicating with a filling port that permits injecting cells through the support into the capsule and the other communicating with another port that allows gas to escape through the support as cells are introduced. The capsule may be made immunoisolatory by controlling membrane permselectivity or by substances in the membrane that reduce immune response, and may contain a tether for retrieval that can be formed integrally with the inner support.

Claims

1. A biocompatible cell capsule for implantation, comprising:

(a) a capsule jacket connected to a chamber top at one end and chamber bottom at the other end enclosing a cell chamber,

(b) the capsule jacket comprising at least one semipermeable surface across which biologically active molecules can be delivered, and

(c) an inner support having two ends and extending through the cell chamber such that one end is connected to the chamber top and other end is connected to the chamber bottom, said inner support providing tensile strength to the capsule, and said inner support comprising a filling means for introducing cells or cell-sustaining substances into said chamber and a vent means for escape of air from said chamber as the cells or cell-sustaining substances are introduced.

2. The capsule of claim 1 wherein the cell chamber top comprises a top sealing fitting and the cell chamber bottom comprises a bottom sealing fitting.

3. The capsule of claim 2 wherein the capsule is tubular, the chamber has a longitudinal axis running between the top sealing fitting and the bottom sealing fitting and wherein the inner support is cylindrical and arranged concentrically with the longitudinal axis.

4. The capsule of claim 1 wherein the inner support is coated with a cell-adhesive substance or a cell-viability-enhancing substance.

5. The capsule of claim 1 wherein the inner support is a tube and wherein the filling means comprises at least one filling port and at least one channel through the tube, the channel having an opening which communicates with the cell chamber, and the channel being aligned concentrically with the at least one filling port.

6. The capsule of claim 1 further comprising a tether for retrieval.

7. The capsule of claim 6 wherein said tether is integrally formed with the inner support.

8. The capsule of claim 1 wherein the capsule is immunoisolatory.

9. The capsule of claim 5 wherein the vent means comprises at least one vent port and at least one channel through the tube, the channel having an opening which communicates with the cell chamber, and the channel being aligned concentrically with the at least one vent port.

10. The capsule of either claim 5 or 9 wherein the inner support is a tube and the filling means and the vent means comprise a first channel and a second channel, respectively, wherein the first channel is aligned concentrically with the filling port, and the second channel is aligned concentrically with the vent port.