Method of PCR testing of pooled blood samples

  • US 5,780,222 A
  • Filed: 01/06/1997
  • Issued: 07/14/1998
  • Est. Priority Date: 04/10/1995
  • Status: Expired due to Term
First Claim
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1. A method for testing a multiplicity of plasma donations to uniquely identify donations having a positive viral indication, in a single PCR testing cycle, the method comprising the steps of:

  • providing a plurality of plasma donations, wherein a portion of each donation is contained in a tubing segment divided along its length by spaced-apart seals, the tubing segment portions between the seals defining sequential containers, wherein each container contains a plasma sample of said donation;

    defining an n-dimensional grid, wherein n is 2 or 3 and, wherein the grid elements are each defined by the intersecting coordinates of the n-dimensions of the grid;

    mapping a sample from particular ones of the plurality of plasma donations to a corresponding one of each element of the grid, each sample being defined by a matrix notation Xrcs, wherein the subscript of the matrix notation defines the dimensional indices of the grid;

    taking n aliquots from each plasma sample of each of the plurality of plasma donations, the number of aliquots taken from each sample defined by the number of dimensional indices comprising the grid;

    forming subpools from the aliquots of each sample, wherein each subpool comprises an aliquot of all samples in which one dimensional index is fixed;

    testing all of the subpools, in a single PCR testing cycle, for a viral indication; and

    evaluating the dimensional indicia of each subpool which tested positive in the single PCR testing cycle, in accordance with a reduction by the method of minors, thereby unambiguously identifying a unique element defined by the dimensional indicia of each positive subpool, thus unambiguously identifying a uniquely positive sample.

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