Biosensor

• US 5,795,774 A
• Filed: 07/10/1997
• Issued: 08/18/1998
• Est. Priority Date: 07/10/1996
• Status: Expired due to Term

• Current Assignee: NEC Corporation
• Original Assignee: NEC Corporation
• Inventors: Furusawa, Masako, Matsumoto, Toru, Ito, Narushi, Nakamoto, Shinya
• Primary Examiner(s): Redding, David A.
• Application Number:
  US08/891,197
• Time in Patent Office: 404 Days
• Field of Search: 435/287.9, 204/400, 204/403, 204/415, 204/416, 204/418
• US Class Current: 204/403.11
• CPC Class Codes: C12Q 1/002 Electrode membranes

Abstract

A drop in measurement accuracy of biosensors, due to interferents in a measurement sample is avoided by an arrangement wherein: a hydrogen peroxide electrode 2 is formed on an insulated base 1, and then a γ-aminopropyltriethoxysilane film 3, an acetylcellulose film 4, a perfluorocarbonsulfonic acid resin film 5, an organic macromolecular film 6 incorporating an immobilized enzyme with catalytic properties, and a polyalkylsiloxane film 7, are sequentially formed on the electrode 2.

Claims

1. A biosensor comprising:

an insulated base;

a hydrogen peroxide electrode formed on the insulated base,

a first film which is formed on the hydrogen peroxide electrode and in which the major constituent is γ

-aminopropyltriethoxysilane;

a second film which is formed on the first film and in which the major constituent is a cellulose derivative;

a third film which is formed on the second film and in which the major constituent is an ion exchange resin having a perfluorocarbon skeleton; and

a fourth film which is formed on the third film and in which the major constituent is an organic macromolecular film incorporating an immobilized enzyme with catalytic properties for producing hydrogen peroxide.

2. A biosensor according to claim 1, further comprising a fifth film which is formed on the fourth film and in which the major constituent is polyalkylsiloxane.

3. A biosensor according to claim 1, wherein the concentration of γ

-aminopropyltriethoxysilane at the time of forming the film is within a range of 0.5˜

2 (v/v %).

4. A biosensor according to claim 1, wherein the cellulose derivative is acetylcellulose, and the concentration of this acetylcellulose at the time of forming the film is within a range of 0.5˜

5 (w/v %).

5. A biosensor according to claim 1, wherein the ion exchange resin based having a perfluorocarbon skeleton is perfluorocarbonsulfonic acid resin, and the concentration of this perfluorocarbonsulfonic acid resin at the time of forming the film is 1 (v/v %) or greater.

6. A biosensor according to claim 1, wherein a solution of glutaraldehyde and albumin is used at the time of forming the organic macromolecular film incorporating an immobilized enzyme with catalytic properties, and the concentration of the glutaraldehyde is within a range of 0.1˜

2 (v/v %).

7. A biosensor according to claim 2, wherein the concentration of polyalkylsiloxane at the time of forming the film is 7.0 (v/v %) or greater.

8. A biosensor according to claim 1, wherein the hydrogen peroxide electrode on the insulated base is made up of a working electrode, a counter electrode, and a reference electrode.

9. A biosensor according to claim 8, wherein two or more working electrodes are formed on the insulated base, and at the same time two or more types of organic macromolecular films incorporating an immobilized enzyme with catalytic properties are formed.