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Enzyme-immobilized electrode, composition for preparation of the same and electrically conductive enzyme

  • US 5,804,047 A
  • Filed: 12/16/1994
  • Issued: 09/08/1998
  • Est. Priority Date: 03/31/1992
  • Status: Expired due to Term
First Claim
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1. A process for producing an electrically conductive enzyme comprising an enzyme and a mediator attached to a side chain and the body of the enzyme through a covalent bond wherein said enzyme is glucose oxidase and said mediator is a ferrocene derivative attached to the side chain of the enzyme through a spacer, comprising the steps ofoxidizing a glucose oxidase with an oxidizing agent to form an aldehyde group on an oligosaccharide of the glucose oxidase;

  • reacting said glucose oxidase with a dihydrazide represented by the following formula (II);

    ##STR5## wherein n is an integer of 1 to 7; and

    reacting the reaction product with a ferrocenecarboxylic acid.

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