Enzyme-immobilized electrode, composition for preparation of the same and electrically conductive enzyme
First Claim
1. A process for producing an electrically conductive enzyme comprising an enzyme and a mediator attached to a side chain and the body of the enzyme through a covalent bond wherein said enzyme is glucose oxidase and said mediator is a ferrocene derivative attached to the side chain of the enzyme through a spacer, comprising the steps ofoxidizing a glucose oxidase with an oxidizing agent to form an aldehyde group on an oligosaccharide of the glucose oxidase;
- reacting said glucose oxidase with a dihydrazide represented by the following formula (II);
##STR5## wherein n is an integer of 1 to 7; and
reacting the reaction product with a ferrocenecarboxylic acid.
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Abstract
An enzyme sensor system capable of quantitatively determining a particular component contained in a biological sample in a rapid and easy manner is disclosed. More particularly, an electrically conductive enzyme, an enzyme-immobilized electrode using the same and a composition for use in the preparation of the enzyme electrode are disclosed. The composition for an enzyme-immobilized electrode according to the present invention enables electrodes of various patterns to be produced by screen printing.
263 Citations
7 Claims
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1. A process for producing an electrically conductive enzyme comprising an enzyme and a mediator attached to a side chain and the body of the enzyme through a covalent bond wherein said enzyme is glucose oxidase and said mediator is a ferrocene derivative attached to the side chain of the enzyme through a spacer, comprising the steps of
oxidizing a glucose oxidase with an oxidizing agent to form an aldehyde group on an oligosaccharide of the glucose oxidase; reacting said glucose oxidase with a dihydrazide represented by the following formula (II);
##STR5## wherein n is an integer of 1 to 7; and
reacting the reaction product with a ferrocenecarboxylic acid.- View Dependent Claims (2, 3, 4, 5, 6)
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7. A process for preparing an enzyme-immobilized electrode which comprises immobilizing a composition on a base material, the composition comprising an electrically conductive enzyme, an electrically conductive component and a vehicle.
Specification