Methods and transformed mammalian lymphocyte cells for producing functional antigen-binding protein including chimeric immunoglobulin

  • US 5,807,715 A
  • Filed: 06/27/1994
  • Issued: 09/15/1998
  • Est. Priority Date: 08/27/1984
  • Status: Expired due to Term
First Claim
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1. A method for producing a functional immunoglobulin comprising a heavy chain and a light chain, which comprises the steps of:

  • (a) transfecting a transformed mammalian lymphocytic cell with a first DNA molecule coding for a first chain of the immunoglobulin;

    (b) transfecting the cell with a second DNA molecule, said second DNA molecule coding for a second chain of the immunoglobulin, said second chain being a chain other than the first chain and said first and second chains being either the heavy chain or the light chain; and

    (c) maintaining the cell in a nutrient medium, so that the cell expresses the first and second DNA molecules and the resultant chains are intracellularly assembled together to form the immunoglobulin which is then secreted in a form capable of specifically binding to antigenwherein prior to step (a) the cell does not express a functional immunoglobulin capable of specifically binding antigen.

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