Multilayer analytical element for the determination of an analyte in a liquid

• US 5,814,522 A
• Filed: 06/20/1996
• Issued: 09/29/1998
• Est. Priority Date: 06/24/1995
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Zimmer, Volker, Lerch, Rolf, Macho, Heinz
• Primary Examiner(s): Alexander, Lyle A.
• Application Number:
  US08/668,059
• Time in Patent Office: 831 Days
• Field of Search: 422/56-58, 422/61, 436/164, 436/169, 436/170
• US Class Current: 436/170
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements

Abstract

A sample application zone and detection zone are arranged on a pile-like complex made of a fleece and a porous membrane which are in a direct or indirect contact that enables passage of liquid through the contact area and the membrane is a polyamide, polyvinylidene difluoride, polyether sulfone or polysulfone membrane which transports liquid significantly slower over the area than the fleece.

Claims

1. Multilayer analytical element for the determination of an analyte in a liquid, said multilayer analytical element comprising an application zone and a detection zone arranged side by side on a stacked complex composed of a fleece and a porous polyamide, polyvinylidene difluoride, polyether sulfone or polysulfone membrane which transports liquid more slowly over its area than said fleece, wherein said fleece and said porous membrane are in fluid contact, via a contact area which permits passage of liquid therethrough, a portion of said porous membrane being in said detection zone and having a detection reagent in it or on it, which forms a detectable signal with said analyte.

2. Multilayer analytical element as claimed in claim 1 wherein the membrane is composed of a small-pore and a large-pore layer which are arranged with the large pore layer facing the fleece.

3. Multilayer analytical element as claimed in claim 1, wherein reagent is located in or on the membrane at a site at which the membrane can take up liquid.

4. Multilayer analytical element as claimed in claim 1, wherein the pores of the membrane have a pore size between 0.01 and 5 μ

m preferably between 0.04 and 3 μ

m.

5. A method for determining an analyte in a liquid, comprising contacting said liquid to the multilayer analytical element of claim 1, and determining signal formed as a determination of said analyte.

6. Multilayer analytical element as claimed in claim 1, wherein the membrane is treated in the sample application zone in such a way that it does not take up any liquid.

7. Multilayer analytical element as claimed in claim 6, wherein the membrane contains reagents for the determination of different analytes which are spatially separated from one another.

8. Method for determination of a analyte in a liquid, comprising

contacting said liquid to a multilayer analytical element which has a sample application zone and a detection zone arranged side by side, said multilayer analytical element comprising a fleece and a porous polyamide, polyvinyl difluoride, polyether sulfone or polysulfone membrane, each of which transports liquid, said porous membrane transporting liquid more slowly than said fleece, wherein a portion of said porous membrane is in said detection zone, and contains a reagent which forms a signal with said analyte and is in fluid contact with said fleece, said fluid contact permitting passage of liquid from said fleece to said porous membrane, said porous membrane, and

determining formation of said signal as determination of said analyte.

9. Multilayer analytical element for determination of an analyte in a liquid sample, said multilayer analytical element comprising a sample application zone and a detection zone arranged side by side, said multilayer analytical element comprising a fleece which transports said liquid sample and a porous polyamide, polyvinylidene difluoride, polyether sulfone, or polysulfone membrane which transports said liquid more slowly than said fleece, wherein a portion of said porous membrane is in said detection zone and contains a reagent which forms a detectable signal with said analyte, wherein said porous membrane is in fluid contact with said fleece, said fluid contact permitting passage of liquid from said fleece to said porous membrane, said porous membrane being a membrane.