Method and apparatus for determining analytical data concerning the inside of a scattering matrix

• US 5,825,488 A
• Filed: 11/08/1996
• Issued: 10/20/1998
• Est. Priority Date: 11/18/1995
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Kohl, Matthias, Cope, Mark, Boecker, Dirk, Essenpreis, Matthias
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): Smith, Zandra V.
• Application Number:
  US08/745,204
• Time in Patent Office: 711 Days
• Field of Search: 356/336, 356/337, 356/342, 128/633, 128/634, 128/665
• US Class Current: 356/342
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

A method and an apparatus for determining analytical data concerning the inside of a scattering matrix, in particular of a biological sample. In a detection step detection measurements are carried out in which light is irradiated into the matrix as primary light at an irradiation site (12) through an interface bounding the scattering matrix (6) and light emerging out of the scattering matrix through the interface is detected as secondary light at a detection site (13) at a predetermined measuring distance from the irradiation site, in order to determine as a measurement variable a measurable physical property of the light which varies due to the interaction of the light with the scattering matrix, which measurement variable is a measure of the analytical data to be determined. In an evaluation step the analytical data are determined on the basis of the measurement variable measured in the detection step. In order that such a matrix analysis may be carried out with relatively simple measuring means, at least two detection measurements are carried out in the detection step with different reflection conditions at the interface (5) between the irradiation site (12) and the detection site (13), in each of which the measurement value of the measurement variable is determined.

Claims

1. A method for determining analytical data regarding a light scattering matrix, said method comprising the steps of:

detecting secondary light by irradiating light into the scattering matrix as primary light at an irradiation site through an interface bounding the scattering matrix, and detecting light emerging out of the scattering matrix through the interface, said emerging light being detected as secondary light at a detection site which is located at a predetermined measuring distance from the irradiation site;

determining a measurement value of a measurable physical property of the secondary light, said measurable physical property varying due to an interaction of the primary light with the scattering matrix;

evaluating the measurement value as a measure of the analytical data to be determined, the analytical data being determined based upon a measurement value of the measurement variable,

wherein at least two of said detecting steps are carried out under different reflection conditions at the interface between the irradiation site and the detection site, and measurement values determined in said at least two detection steps are evaluated in said evaluation step to determine said analytical data.

2. A method according to claim 1, wherein the measurement variable is an intensity of the secondary light at the detection site.

3. A method according to claim 1, wherein said evaluation step comprises a step of forming a ratio of at least two measurement values formed during the at least two detecting steps.

4. A method according to claim 1, wherein said at least two detecting steps are performed with different measuring distances between the irradiation site and the detection site.

5. A method according to claim 1, wherein the at least two detecting steps are performed with a same measuring distance between the irradiation site and the detection site.

6. A method according to claim 1, wherein said at least two detecting steps are performed with reflective surfaces having differing reflectivities arranged between the respective irradiation site and the detection site at the interface bounding the biological sample.

7. A method according to claim 6, wherein the different reflectivities of the at least two detecting steps are formed by changing the reflectivity of a reflecting surface.

8. A method according to claim 6, wherein one of a first irradiation site and a first detection site in a first of said at least two detecting steps is different than one of a second irradiation site and a second detection site of a second of said at least two detecting steps, so that different sections of the interface are disposed between the respective irradiation and detection sites in said first and second detection steps and said different sections have respective reflectivities of different values.

9. A method according to claim 1, wherein the analytical data includes an absorption parameter which corresponds to a measure of the optical absorption of the primary light in the scattering matrix.

10. A method according to claim 1, wherein the analytical data includes a scattering parameter which corresponds to a scattering of the primary light in the scattering matrix.

11. A method according to claim 10, wherein the light scattering matrix is a biological sample.

12. A method according to claim 11, wherein the analytical data includes a concentration of an analyte in the biological sample.

13. A method according to claim 12, wherein a concentration of an analyte in the biological sample is determined based upon the absorption parameter.

14. A method according to claim 12, wherein a concentration of an analyte in the biological sample is determined based upon the scattering parameter.

15. A method according to claim 1, wherein the biological sample comprises skin tissue.

16. A method according to claim 1, wherein the biological sample comprises subcutaneous fatty tissue.

17. A method according to claim 1, wherein the biological sample comprises sclerae tissue.

18. An apparatus for determining analytical data of a light scattering matrix, said apparatus comprising:

a measuring head, said measuring head including a sample contact surface for contacting an interface of the biological sample;

light irradiation means disposed in said measuring head for irradiating light into the biological sample at an irradiation site through an irradiation opening in the sample contact surface,

detection means connected to said measuring head for detecting secondary light emerging from the scattering matrix at a detection site through a detection opening in the sample contact surface;

evaluation means connected to said light irradiation means and said detection means for determining analytical data based upon the primary light and the secondary light,

wherein said sample contact surface includes reflection adjustment means wherein different reflection conditions can be provided in at least two detection measurements.

19. An apparatus according to claim 18, wherein said reflection adjustment means comprises a liquid crystal display.

20. An apparatus according to claim 18, wherein said sample contact surface includes at least two irradiation openings, such that a first section of said sample contact surface is disposed between a first of said at least two irradiation openings and the detection opening, and a second section of said sample contact surface is diposed between a second of said at least two irradiation openings and the detection opening, said first section and said second section having respective reflectivities of different values.

21. An apparatus according to claim 18, wherein said sample contact surface includes at least two detection openings wherein a first section of the sample contact surface is disposed between the irradiation opening and a first of the at least two detection openings, and a second section of the sample contact surface is disposed between the irradiation opening and a second of the at least two detection openings, and wherein the first section and the second section have respective reflectivities of different values.