Expression of polypeptides in yeast

  • US 5,856,123 A
  • Filed: 06/06/1995
  • Issued: 01/05/1999
  • Est. Priority Date: 02/25/1981
  • Status: Expired due to Term
First Claim
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1. A process of forming a transformant of a yeast strain capable of expressing a polypeptide ordinarily exogenous to yeast, comprising transforming said yeast strain with a DNA transfer vector that comprises:

  • (a) bacterial and yeast origins of replication and genes for phenotypic selection of both bacterial and yeast moieties transformed with said vector;

    (b) a DNA fragment comprising a structural gene encoding said polypeptide;

    (c) a yeast promoter genetically distinct from said structural gene obtained from the about 1500 bp DNA sequence 5'"'"' flanking the start signal of a yeast structural gene; and

    (d) translation start and stop signals for said structural gene encoding said polypeptide that are positioned so that said structural gene encoding said polypeptide is under the control of said promoter while maintaining said origins of replications and genes for phenotypic selection.

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