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Fluorescence imaging system and method

  • US 5,865,754 A
  • Filed: 08/23/1996
  • Issued: 02/02/1999
  • Est. Priority Date: 08/24/1995
  • Status: Expired due to Term
First Claim
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1. A method of imaging, comprising the steps of:

  • (a) exposing a surface of a light scattering material to an excitation light from a light source, the material having a heterogeneous composition under the surface;

    (b) detecting a fluorescent emission from the material in response to step (a);

    (c) establishing an estimate of spatial variation of a fluorescence characteristic of the material;

    (d) determining a calculated emission as a function of the estimate;

    (e) comparing the calculated emission to the emission detected in step (b) to determine an error;

    (f) providing a modified estimate of spatial variation of the fluorescence characteristic and repeating steps (d) through (f) until the error reaches a desired minimum; and

    (g) generating an image of the material from the modified estimate, the image corresponding to the heterogeneous composition of the material.

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