Transgenic mice expressing APPK670N,M671L and a mutant presenilin transgenes

  • US 5,898,094 A
  • Filed: 07/30/1997
  • Issued: 04/27/1999
  • Est. Priority Date: 10/21/1996
  • Status: Expired due to Term
First Claim
Patent Images

1. A transgenic mouse with enhanced Alzheimer'"'"'s Disease related amyloid accumulation in its brain produced by:

  • producing an F1 generation mouse by crossing a first transgenic mouse whose genome comprises at least one transgene comprising a DNA sequence encoding mutant presenilin M146L operably linked to a promoter with a second transgenic mouse whose genome comprises at least one transgene comprising a DNA sequence encoding APP K670N,M671L operably linked to a promoter, wherein the first transgenic mouse expresses the DNA sequence encoding the mutant presenilin and wherein the second transgenic mouse expresses the DNA sequence encoding the APP; and

    selecting from the offspring of the cross, those transgenic mice whose genome comprises at least one DNA sequence encoding mutant presenilin M146L operably linked to a promoter and at least one transgene comprising a DNA sequence encoding APP K670N,M671L operably linked to a promoter, and identifying an F1 mouse which express both transgenes such that the F1 mouse develops accelerated deposition of Aβ

    in its brain as compared to non-transgenic mice or either parental mouse.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×