Bead linkers for immobilizing nucleic acids to solid supports

• US 5,900,481 A
• Filed: 11/06/1996
• Issued: 05/04/1999
• Est. Priority Date: 11/06/1996
• Status: Expired due to Term

• Current Assignee: Agena Bioscience Inc. (Mesa Laboratories Inc.)
• Original Assignee: Sequenom Incorporated (Laboratory Corporation Of America Holdings)
• Inventors: Lough, David M., Koster, Hubert
• Primary Examiner(s): Kunz, Gary L.
• Application Number:
  US08/746,036
• Time in Patent Office: 909 Days
• Field of Search: 536/55.3, 536/56, 536/123.1, 502/233, 525/332.2
• US Class Current: 506/30
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00504 Pins
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: C07F 9/2408 of hydroxyalkyl compounds
• CPC Class Codes: C07F 9/2429 of arylalkanols
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6816 : characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6827 : for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6837 : using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6858 : Allele-specific amplification
• CPC Class Codes: C12Q 1/6872 : involving mass spectrometry
• CPC Class Codes: C12Q 1/6883 : for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6886 : for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2563/149 : Particles, e.g. beads
• CPC Class Codes: C12Q 2565/627 : being a mass spectrometer
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 2035/1069 : by adjusting the spacing between multiple probes of a single transferring head
• CPC Class Codes: G01N 35/1067 : for transfer to or from containers having different spacing

Abstract

Novel compositions comprised of at least one bead conjugated to a solid support and further conjugated to at least one nucleic acid and preferred methods for making the novel compositions are described. As compared to "flat" surfaces, beads linked to a solid support provide an increased surface area for immobilization of nucleic acids. Furthermore, by selecting a bead with the desired functionality, a practitioner can select a functionalization chemistry for immobilizing nucleic acids, which is different from the chemistry of the solid support.

Claims

1. A composition comprising a bead conjugated to a solid support and further conjugated to a nucleic acid.

2. A composition of claim 1, wherein the bead is made from a material selected from the group consisting of:

silica gel, glass, magnet, Wang resin, Merrifield resin, metal, plastic, cellulose, Sephadex, and Sepharose.

3. A composition of claim 1, wherein the bead is swellable.

4. A composition of claim 1, wherein the bead is nonswellable.

5. A composition of claim 1, wherein the bead is in the range of 1 to 100 μ

m in diameter.

6. A composition of claim 1, wherein the solid support is selected from the group consisting of:

beads, capillaries, plates, membranes, wafers, combs, pins, wafers and wafers with pits.

7. A composition of claim 1, wherein the nucleic acid is DNA.

8. A composition of claim 1, wherein the nucleic acid is RNA.

9. A process of making a bead conjugated to a solid support and further conjugated to a nucleic acid, comprising the steps of conjugating a bead to a nucleic acid;

and conjugating a bead to a solid support.

10. A process of claim 9, wherein the bead is functionalized.

11. A process of claim 10, wherein the bead is functionalized with carboxy functional groups.

12. A process of claim 10, wherein the bead is functionalized with amino functional groups.

13. A process of claim 10, wherein the bead is conjugated to the nucleic acid prior to conjugation of the bead to the solid support.

14. A process of claim 10, wherein the bead is conjugated to the nucleic acid after the bead is conjugated to the solid support.